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Microenvironment and cell mechanics

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BIOcell 2022年第7期46卷 1629-1632页

作者： VAN-CHIEN BUI Institute for Immunology and Transfusion Medicine University Medicine Greifswald17475GreifswaldGermany

Microenvironment contains biophysical and biochemical elements to maintain survival,growth,proliferation,and differentiation of *** change can lead to cell response to the mechanical forces,which can be described by *** is an indicator of a cell’s state since it plays an important role in many cellular *** many cases,cell elasticity is measured by using discontinuous manner,which may not allow elucidating real-time activity of individual live cells in physiological condition or cell response against microenvironmental changes.I argue that measuring cell elasticity using continuously repetitive nanoindentation technique is important that should be *** an example,I discuss mechanics of human embryonic kidney(HEK)cells in various *** resting cells,there is an activity of the cytoskeleton whose oscillation amplitude is strongly affected by the intracellular calcium,and the collective activity of myosin motor proteins induces elasticity *** results also reveal that actin cytoskeleton and cell membrane determine cell mechanics.

关键词： Microenvironment cell mechanics Nanoindentation

Magnetic Nanoparticles for the Quantification of cell mechanics Using Force-Induced Remnant Magnetization Spectroscopy

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Magnetic Nanoparticles for the Quantification of Cell Mechan...

Mechanopathology of red blood cell diseases—Why mechanics matters

第十一届全国化学生物学学术会议

作者： Min Xu Li Yao State Key Laboratory for Structural Chemistry of Unstable and Stable Species Institute of ChemistryChinese Academy of Science University of Chinese Academy of Sciences

Magnetic nanoparticles（MNPs） have emerged as powerful tools for MRI, drug delivery, synergistic chemotherapy and so on1. Recently, a MNPs-based force-induced remnant magnetization spectroscopy（FIRMS） have been used to identify molecular interactions of antigen-antibody, DNA-DNA, aptamer-protein and so on2. Here we use FIRMS to measure cell mechanics. We first investigeted the MNPs-cell interactions from cell mechanics perspective and then use MNPs to label cells, subsequently applied an external centrifugal force with increasing amplitude to the labeled cells. When the applied force exceeds the cell adhesion force, cell detached from substrates, resulting in the decrease of the remanence signal. Therefore, a curve of force-dependent signal change is recorded by FIRMS and the cell adhesion force is obtained.

关键词： Magnetic Nanopaticles Biocompatibility cell mechanics FIRMS

来源： cnki会议评论

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Theoretical & Applied mechanics Letters 2011年第1期1卷 1-6页

作者： Chwee Teck Lim Mechanobiology Institute National University of Singapore Division of Bioengineering and Department of Mechanical Engineering National University of Singapore Singapore-MIT Alliance for Research & Technology(SMART)

During the onset of a disease a cell may experience alterations in both the composition and organization of its cellular and molecular *** alterations may eventually lead to changes in its geometrical and mechanical properties such as cell size and shape,deformability and *** such,knowing how diseased cells respond to mechanical forces can reveal ways by which they differ from healthy ***,we will present biomechanistic insights into red blood cell related diseases that manifest mechanical property changes and how they directly contribute to the pathophysiology of *** conducting cell and molecular mechanics studies,not only can we elucidate changes in the structure-property-function relationship of diseased cells,we can also exploit the new knowledge gained to develop biomechanics based devices that may better detect and diagnose these diseases as well as help identify important biomechanical targets for possible therapeutic interventions.

关键词： human diseases pathophysiology cell mechanics single molecule biomechanics

ANALYSIS OF PULSATILE FLOW IN THE PARALLEL-PLATE FLOW CHAMBER WITH SPATIAL SHEAR STRESS GRADIENT

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Journal of Hydrodynamics 2007年第1期19卷 113-120页

作者： QIN Kai-rong HU Xu-qu LIU Zhao-rong Department of Engineering mechanics Shanghai Jiaotong University Shanghai 200240 China Institute of Biomechanics Fudan University Shanghai 200433 China

The Parallel-Plate Flow Chamber （PPFC）, of which the height is far smaller than its own length and width, is one of the main apparatus for the in-vitro study of the mechanical behavior of cultured vascular Endothelical cells （ECs） exposed to fluid shear stress. The steady flow in different kinds of PPFC has been extensively investigated, whereas, the pulsatile flow in the PPFC has received little attention. In consideration of the characteristics of geometrical size and pulsatile flow in the PPFC, the 3-D pulsatile flow was decomposed into a 2-D pulsatile flow in the vertical plane, and an incompressible plane potential flow in the horizontal plane. A simple method was then proposed to analyze the pulsatile flow in the PPFC with spatial shear stress gradient. On the basis of the method, the pulsatile fluid shear stresses in several reported PPFCs with spatial shear stress gradients were calculated. The results were theoretically meaningful for applying the PPFCs in-vitro, to simulate the pulsatile fluid shear stress environment, to which cultured ECs were exposed.

关键词： Pulsatile flow fluid shear stress spatialshear stress gradient parallel-plate flow chamber culturedendothelial cells cell mechanics

OPTICALLY TRACKING THE MOTION OF MICROBEADS TO STUDY PHYSICAL BEHAVIORS OF THE LIVING cell IN RESPONSE TO TRANSIENT STRETCH OR COMPRESSION

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Journal of Innovative Optical Health Sciences 2011年第2期4卷 143-150页

作者： LINHONG DENG XUEMEI JIANG CHENG CHEN AIJING SONG FENG LIN Key Laboratory of Biorheological Science and Technology Ministry of Education Chongqing UniversityChongqing 400044China

Optical magnetic twisting cytometry and traction force microscopy are two advanced cell mechanics research tools that employ optical methods to track the motion of microbeads that are either bound to the surface or embedded in the substrate underneath the *** former measures rheological properties of the cell such as cell stiffness,and the latter measures cell traction force *** we describe the principles of these two cell mechanics research tools and an example of using them to study physical behaviors of the living cell in response to transient stretch or *** demonstrate that,when subjected to a stretchunstretch manipulation,both the stiffness and traction force of adherent cells promptly reduced,and then gradually recover up to the level prior to the *** staining and Western blotting results indicate that the actin cytoskeleton of the cells underwent a corresponding disruption and reassembly process almost in step with the changes of cell ***,when subjected to compression,the cells did not show such particular *** together,we conclude that adherent cells are very sensitive to the transient stretch but not transient compression,and the stretch-induced cell response is due to the dynamics of actin polymerization.

关键词： Optical tracking magnetic twisting microbeads motion stiffness traction force cell mechanics

cell AND MOLECULAR BIOmechanics: PERSPECTIVES AND CHALLENGES

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Acta Mechanica Solida Sinica 2011年第1期24卷 27-51页

作者： Baohua Ji Gang Bao Department of Applied mechanics Beijing Institute of Technology Beijing 100081 China Department of Biomedical Engineering Georgia Institute of Technology and Emory University Atlanta GA 30332 USA

As an intriguing interdisciplinary research field, cell and molecular biomechanics is at the cutting edge of mechanics in general and biomechanics in particular. It has the potential to provide a quantitative understanding of how forces and deformation at tissue, cellular and molecular levels affect human health and disease. In this article, we review the recent advances in cell and molecular biomechanics, examine the available computational and experimental tools, and discuss important issues including protein deformation in mechanotransduction, cell deformation and constitutive behavior, cell adhesion and migration, and the associated models and theories. The opportunities and challenges in cell and molecular biomechanics are also discussed. We hope to provide readers a clear picture of the current status of this field, and to stimulate a broader interest in the applied mechanics community.

关键词： molecular biomechanics cell mechanics protein mechanics cell adhesion cell migration mechanotransduction

Fluid and Osmotic Pressure Balance and Volume Stabilization in cells Dedicated to Professor Karl Stark Pister for his 95th birthday

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Computer Modeling in Engineering & Sciences 2021年第12期129卷 1329-1350页

作者： Peter M.Pinsky Department of Mechanical Engineering Stanford UniversityStanford94305CAUSA

A fundamental problem for cells with their fragile membranes is the control of their *** primordial solution to this problem is the active transport of ions across the cell membrane to modulate the intracellular osmotic *** this work,a theoretical model of the cellular pump-leak mechanism is proposed within the general framework of linear nonequilibrium *** model is expressed with phenomenological equations that describe passive and active ionic transport across cell membranes,supplemented by an equation for the membrane potential that accounts for the electrogenicity of the ionic *** active ionic transport,the model predicts that the intracellular fluid pressure will be balanced by the osmotic pressure and a new pressure component that arises from the active ionic fluxes.A model for the pump-leak mechanism in an idealized human cell is introduced to demonstrate the applicability of the proposed theory.

关键词： Pump-leak mechanism cell volume regulation active ion transport ion pump membrane transport cell mechanics modified Kedem-Katchalsky equations nonequilibrium thermodynamics phenomenological equations

High-throughput physical phenotyping of cell differentiation

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Microsystems & Nanoengineering 2017年第1期3卷 316-322页

作者： Jonathan Lin Donghyuk Kim Henry T.Tse Peter Tseng Lillian Peng Manjima Dhar Saravanan Karumbayaram Dino Di Carlo Department of Bioengineering University of CaliforniaLos AngelesCA 90095USA CytoVale Inc. 384 Oyster Point Boulevard#7 SouthSan FranciscoCA 94080USA Department of Biomedical Engineering Tufts UniversityMedfordMA 02155USA Eli and Edythe Broad Center of Regenerative Medicine and Stem cell Research Los AngelesCA 90095USA California NanoSystems Institute Los AngelesCA 90095USA Jonsson Comprehensive Cancer Center Los AngelesCA 90095USA Department of Mechanical Engineering University of CaliforniaLos AngelesCA 90095USA

In this report,we present multiparameter deformability cytometry(m-DC),in which we explore a large set of parameters describing the physical phenotypes of pluripotent cells and their derivatives.m-DC utilizes microfluidic inertial focusing and hydrodynamic stretching of single cells in conjunction with high-speed video recording to realize high-throughput characterization of over 20 different cell motion and morphology-derived *** extracted from videos include size,deformability,deformation kinetics,and *** train support vector machines that provide evidence that these additional physical measurements improve classification of induced pluripotent stem cells,mesenchymal stem cells,neural stem cells,and their derivatives compared to size and deformability *** addition,we utilize visual interactive stochastic neighbor embedding to visually map the high-dimensional physical phenotypic spaces occupied by these stem cells and their progeny and the pathways traversed during *** report demonstrates the potential of m-DC for improving understanding of physical differences that arise as cells differentiate and identifying cell subpopulations in a label-free ***,such approaches could broaden our understanding of subtle changes in cell phenotypes and their roles in human biology.

关键词： cell mechanics deformation deformation kinetics morphology physical phenotype stem cells

Modeling of micropipette aspiration and optical tweezers stretching of erythrocytes with or without Malaria parasite

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Theoretical & Applied mechanics Letters 2013年第3期3卷 59-64页

作者： Guyue Jiao Ruojing Zhang School of Aerospace Engineering and Applied mechanics Tongji University

The erythrocytes play an important role in the human body. The healthy erythrocytes can undergo extremely large deformation while passing through small capillaries. Their infection by Malaria Plasmodium falcipurum （P.f.） will lead to capillary blockage and blood flow obstruction. Many experimental and computational methods have been applied to study the increase in stickiness and decrease in deformability of the Malaria （P.f.） infected erythrocytes. The novelty of this paper lies in the establishment of an multi-component model for investigating mechanical properties of Malaria （P.f.） infected erythrocytes, especially of their enclosed parasites. Finite element method was applied to simulate the erythrocytes＇ deformation in micropipette aspiration and optical tweezers stretching using the computational software ABAQUS. The comparisons between simulations and experiments were able to quantitatively conclude the effects of stiffness and stickiness of the parasitophorous vacuole membrane on the cells＇ deformation, which could not be obtained from experiments directly.

关键词： cell mechanics Malaria infected red blood cells micropipette aspiration finite elementmethod hyperelasticity