Magnetic Nanoparticles for the Quantification of Cell Mechanics Using Force-Induced Remnant Magnetization Spectroscopy
作者单位：State Key Laboratory for Structural Chemistry of Unstable and Stable SpeciesInstitute of ChemistryChinese Academy of Science University of Chinese Academy of Sciences
会议名称：《第十一届全国化学生物学学术会议》
会议日期：2019年
学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071009[理学-细胞生物学] 09[农学] 070302[理学-分析化学] 0703[理学-化学] 0901[农学-作物学] 090102[农学-作物遗传育种]
关 键 词：Magnetic Nanopaticles Biocompatibility Cell Mechanics FIRMS
摘 要：Magnetic nanoparticles（MNPs） have emerged as powerful tools for MRI, drug delivery, synergistic chemotherapy and so on1. Recently, a MNPs-based force-induced remnant magnetization spectroscopy（FIRMS） have been used to identify molecular interactions of antigen-antibody, DNA-DNA, aptamer-protein and so on2. Here we use FIRMS to measure cell mechanics. We first investigeted the MNPs-cell interactions from cell mechanics perspective and then use MNPs to label cells, subsequently applied an external centrifugal force with increasing amplitude to the labeled cells. When the applied force exceeds the cell adhesion force, cell detached from substrates, resulting in the decrease of the remanence signal. Therefore, a curve of force-dependent signal change is recorded by FIRMS and the cell adhesion force is obtained.