High-throughput physical phenotyping of cell differentiation

作     者：Jonathan Lin Donghyuk Kim Henry T.Tse Peter Tseng Lillian Peng Manjima Dhar Saravanan Karumbayaram Dino Di Carlo 

作者机构：Department of BioengineeringUniversity of CaliforniaLos AngelesCA 90095USA CytoVale Inc.384 Oyster Point Boulevard#7 SouthSan FranciscoCA 94080USA Department of Biomedical EngineeringTufts UniversityMedfordMA 02155USA Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell ResearchLos AngelesCA 90095USA California NanoSystems InstituteLos AngelesCA 90095USA Jonsson Comprehensive Cancer CenterLos AngelesCA 90095USA Department of Mechanical EngineeringUniversity of CaliforniaLos AngelesCA 90095USA 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2017年第3卷第1期

页      面：316-322页

核心收录：

学科分类：0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 100214[医学-肿瘤学] 0811[工学-控制科学与工程] 0702[理学-物理学] 10[医学] 

基　　金：We acknowledge financial support from the Packard Foundation and the National Science Foundation grant no.1150588 

主　　题：cell mechanics deformation deformation kinetics morphology physical phenotype stem cells 

摘      要：In this report,we present multiparameter deformability cytometry(m-DC),in which we explore a large set of parameters describing the physical phenotypes of pluripotent cells and their derivatives.m-DC utilizes microfluidic inertial focusing and hydrodynamic stretching of single cells in conjunction with high-speed video recording to realize high-throughput characterization of over 20 different cell motion and morphology-derived *** extracted from videos include size,deformability,deformation kinetics,and *** train support vector machines that provide evidence that these additional physical measurements improve classification of induced pluripotent stem cells,mesenchymal stem cells,neural stem cells,and their derivatives compared to size and deformability *** addition,we utilize visual interactive stochastic neighbor embedding to visually map the high-dimensional physical phenotypic spaces occupied by these stem cells and their progeny and the pathways traversed during *** report demonstrates the potential of m-DC for improving understanding of physical differences that arise as cells differentiate and identifying cell subpopulations in a label-free ***,such approaches could broaden our understanding of subtle changes in cell phenotypes and their roles in human biology.