Serological investigation of vector-borne disease in dogs from rural areas of China
Serological investigation of vector-borne disease in dogs from rural areas of China作者机构：National Institute of Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention Department of Infectious Diseases302 Hospital of PLA
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2012年第2卷第2期
页 面：102-103页
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学]
基 金：supported by the National Basic Research Program of China(973 Program)2010CB530200(2010CB530206) the China-US Collaborative Program on Emerging and Re-emerging Infectious Disease(No.1U2GGH000018-01)
主 题：Anaplasma phagocytophilum Ehrlichia canis Borrelia burgdorferi Dirofilaria immitis Dogs China Vector-borne disease Serological investigation ELISA rapid diagnostic assay
摘 要：Objective:To evaluate the Anaplasma phagocytophilum(A.phagocytophilum),Ehrlichia canis(E.canis,Dirofilaria immitis(D.immitis)(canine heartworm),Borrelia burgdorferi(B.burgdorferi)infections in countryside dogs from Yunnan,Hainan and Anhui provinces.Methods:Serum samples were collected from 26 dogs in Yunnan.Hainan and Anhui provinces.The samples were tested using a commercial ELISA rapid diagnostic assay kit(SNAP^(?)4Dx^(?);IDEXX Laboratories,Inc.U.S.A.).Meaiiwliile,indirect immunofluorescence assay(IFA)recommended by WHO was conducted to delect IgG to A.phagocytophilum.Two methods were analyzed and compared.Results:The number of serologically positive dogs for IgG to A.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs responded positive for E.canu.D.immitis(canine heartworm,and B.burgdorferi by ELISA rapid diagnostic method.The number of serologically positive dogs for IgG to A.phagocytophilum was 13(50%)by IFA method.Data of the two methods were analyzed by statistical software and the difference was statistically significant(P=0.002).Conclusions:It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method.However,we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including.***.phagocytophilum,E.canis,D.immitis(canine heartworm),and B.burgdorferi which have public health significance.
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