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Biomedical and Environmental Sciences 2019年第5期32卷 315-323页

作者： WANG Dan Dan YI Lin Zhu WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping Department of Epidemiology Shanxi Medical UniversityTaiyuan 030001ShanxiChina Department of Obstetrics and Gynaecology the Third People’s Hospital of Taiyuan CityTaiyuan 030001ShanxiChina

Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV Serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV Serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P Serological markers could be combined with PBMC‐related markers in prenatal testing.

关键词： Hepatitis B virus Intrauterine transmission Peripheral blood mononuclear cells Covalently closed circular deoxyribonucleic acid Serological markers

Nested case-control study of multiple Serological indexes and Brighton pediatric early warming score in predicting death of children with sepsis

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World Journal of Clinical Cases 2019年第4期7卷 431-440页

作者： Xiong Xie Ming Li Tian-Tian Xiong Rui Wang Liang Xiao Department of Pediatrics Third Clinical Hospital China Three Gorges University Gezhouba Central Hospital

BACKGROUND Currently, it is difficult to predict the complications of children at the early stage of sepsis. Brighton pediatric early warning score(PEWS) is a disease risk assessment system that is simple and easy to operate, which has good sensitivity and specificity in disease recognition among children. Because detection indicators vary widely in children, a single indicator is difficult to assess the posttreatment status of children with sepsis.AIM To investigate the relationship between Serological markers, Brighton PEWS, and death in children with sepsis after treatment.METHODS A total of 205 children diagnosed with sepsis at our hospital were enrolled. The baseline data, serum scores, and PEWS scores were recorded. In the nested casecontrol study, children who died during the study period were included in an observation group. According to the matching principle, the children who were not dead in the same cohort were included in a control group. The influencing factors of death in children with sepsis after treatment and the value of each evaluation index in predicting the prognosis of children were analyzed.RESULTS A total of 96 children were enrolled in the study, including 48 each in the observation group and the control group. Multivariate logistic regression analysis indicated that antibacterial treatments within 1 h(P = 0.017), shock(P = 0.044),multiple organ dysfunction syndrome(MODS)(P = 0.027), serum procalcitonin(PCT)(P = 0.047), serum albumin(ALB)(P = 0.024), and PEWS(P = 0.012) were independent risk factors for the death of children with sepsis. The area under the curve of the combination of ALB, PCT, and PEWS to predict the death in children with sepsis was the highest(0.908).CONCLUSION Antibacterial treatments within 1 h, shock, MODS, PCT, ALB, and PEWS are independent risk factors for the death of children with sepsis. The predictive accuracy of the combination of PCT, ALB, and PEWS for the prognosis of children with sepsis is the best.

关键词： Serological indicators Pediatric early warming score Sepsis Nested casecontrol study Systemic inflammatory reaction syndrome

STUDIES ON SPECIFIC Serological ANTIGENS IN METACERCARIAE AND JUVENILES OF Paragoniums westermani AND ITS MONOCLONAL ANTIBODIES

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Science China Chemistry 1990年第2期33卷 178-187页

作者：蒋作君沈一平赵慰先 Nanjing Medical College Nanking 210005 PRC

The polyclonal antibodies to juveniles of Paragoniums westermani (PwJ-PcAbs) from sera of Wistar rats infected with Paragoniums westerrnani (P. w.)were purified by Sephadex G 200 chromatography. Next the shared Serological antigens of P. w. metacercaria and juveniles (PwMJ-SAg) from the crude antigens of the metacercariae (M-NS-Ag) were purified with immuno-affinity chromatography on cyanogen bromide-activated cross-linked Sepharose 4B beads coupled withPwJ-PcAbs. PwMJ-SAg, agroup of glycoprotein molecules shown by the staining test, were specific Serological antigens of P. w. metacercariae and juveniles, identified by the immunoabsorb test and immunoelectrophoresis. By SDSPAGE, PwMJ-SAg were fractionated to seven bands, including major bands A(27.SK) and Bi(19.5 K), the two major Serological antigen molecules. 20 sera samples from the patients with the nonpulmonary type of P. w. paragonimiasis were detected using PwMJ-SAg and M-NS-Ag by Dot-ELISA, and the difference of sensitivity between two

关键词： paragonimiasis Serological antigens monoclonal antibodies diagnosis.

Monoclonal antibody-based Serological detection of potato virus M in potato plants and tubers

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Journal of Integrative Agriculture 2020年第5期19卷 1283-1291页

作者： ZHANG Yu GAO Yan-ling HE Wan-qin WANG Ya-qin QIAN Ya-juan ZHOU Xue-ping WU Jian-xiang State Key Laboratory of Rice Biology Institute of BiotechnologyZhejiang UniversityHangzhou 310058P.R.China Potato Research Institute of Heilongjiang Academy of Agricultural Sciences Harbin 150086P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Potato virus M(PVM) is one of the common and economically important potato viruses in potato-growing regions worldwide. To investigate and control this viral disease, efficient and specific detection techniques are needed. In this study, PVM virions were purified from infected potato plants and used as the immunogen to produce hybridomas secreting PVM-specific monoclonal antibodies(MAbs). Four highly specific and sensitive murine MAbs, i.e., 1 E1, 2 A5, 8 A1 and 17 G8 were prepared through a conventional hybridoma technology. Using these four MAbs, we have developed an antigen-coated plate(ACP)-ELISA, a dot-ELISA and a Tissue print-ELISA for detecting PVM infection in potato plants and tubers. PVM could be detected in infected potato plant tissue crude extracts diluted at 1:10 240(w/v, g mL^(–1)) by the dot-ELISA or at 1:163 840(w/v, g mL^(–1)) by the ACP-ELISA. The Tissue print-ELISA is the quickest and easiest assay among the three established Serological assays and is more suitable for onsite large-scale sample detection. Detection results of the field-collected samples showed that PVM is currently widespread in the Yunnan and the Heilongjiang provinces in China. The field sample test results of the developed Serological assays were supported by the results from RT-PCR and DNA sequencing. We consider that the newly established ACP-ELISA, dot-ELISA and Tissue print-ELISA can benefit PVM detection in potato plant and tuber samples and field epidemiological studies of PVM. These assays can also facilitate the production of virus-free seed potatoes and breeding for PVM-resistant potato cultivars, leading to the successful prevention of this potato viral disease.

关键词： potato virus M monoclonal antibody Serological assay antigen-coated plate(ACP)-ELISA dot-ELISA Tissue print-ELISA

Serological investigation of vector-borne disease in dogs from rural areas of China

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Asian Pacific Journal of Tropical Biomedicine 2012年第2期2卷 102-103页

作者： Shiwen Wang Jing He Lijuan Zhang National Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Department of Infectious Diseases 302 Hospital of PLA

Objective:To evaluate the Anaplasma phagocytophilum(A.phagocytophilum),Ehrlichia canis(E.canis,Dirofilaria immitis(D.immitis)(canine heartworm),Borrelia burgdorferi(B.burgdorferi)infections in countryside dogs from Yunnan,Hainan and Anhui provinces.Methods:Serum samples were collected from 26 dogs in Yunnan.Hainan and Anhui provinces.The samples were tested using a commercial ELISA rapid diagnostic assay kit(SNAP^(?)4Dx^(?);IDEXX Laboratories,Inc.U.S.A.).Meaiiwliile,indirect immunofluorescence assay(IFA)recommended by WHO was conducted to delect IgG to A.phagocytophilum.Two methods were analyzed and compared.Results:The number of Serologically positive dogs for IgG to A.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs responded positive for E.canu.D.immitis(canine heartworm,and B.burgdorferi by ELISA rapid diagnostic method.The number of Serologically positive dogs for IgG to A.phagocytophilum was 13(50%)by IFA method.Data of the two methods were analyzed by statistical software and the difference was statistically significant(P=0.002).Conclusions:It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method.However,we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including.***.phagocytophilum,E.canis,D.immitis(canine heartworm),and B.burgdorferi which have public health significance.

关键词： Anaplasma phagocytophilum Ehrlichia canis Borrelia burgdorferi Dirofilaria immitis Dogs China Vector-borne disease Serological investigation ELISA rapid diagnostic assay

Relationship between the high frequency of 23S rRNA point mutations in Treponema pallidum and low Serological response rate to azithromycin treatment in China

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International Journal of Dermatology and Venereology 2019年第1期2卷 6-14页

作者： Rui-Li Zhang Qian-Qiu Wang Zhi-Ju Zheng Guo-Jun Liang Li-Jia Yang Li-Gang Yang Dong-Nu Pei Shao-Chun Lin Department of Dermatology Affiliated Wuxi No.2 People's Hospital of Nanjing Medical UniversityWuxiJiangsu 214002China National Center for STD Control and Prevention China Centers for Diseases Control and PreventionHospital for Skin Diseases(Institute of Dermatology)Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China Department of Dermatology Dermatology Hospital of Southern Medical UniversityGuangzhouGuangdong 510091China Department of Dermatology Hainan Provincial Dermatology HospitalHaikouHainan 570100China Department of Dermatology Institute of DermatologySichuan Provincial People's HospitalChengduSichuan 611500China

Objective Although azithromycin is effective against Treponema pallidum (T.pallidum),the causative agent of syphilis,recent reports indicate that the prevalence of azithromycin resistance in China is very high,which may result in the failure of treatment.In this study,we aimed to investigate the association between azithromycin resistance and therapeutic outcomes in early syphilis patients.Methods Between February 2010 and December 2014,patients aged 18-65 years with early syphilis were enrolled.T.pallidum DNA were extracted to test the presence of A2058G and A2059G mutations.Then,eligible patients were randomly assigned to receive oral azithromycin (0.5 g,once daily for 15 days) or intramuscular BPG (2.4 million units,once weekly for 3 weeks).All patients were followed up in 2 weeks and 3,6,9,and 12 months after treatment to collect demographic and clinical characteristics and laboratory results.The differences on Serological response,Serological failure and serofast rate were compared between the two groups by Chi-square test.Results Among the 187 T.pallidum-infected patients enrolled,172 (92.0%) cases had a mutation associated with azithromycin resistance (A2058G,153 cases;A2059G,19 cases).During the 5-year study period,the percentage of cases enrolled with these mutations steadily increased,from 90.9% in 2010 to 95.3% in 2014.Of the 172 patients presenting with these mutations,only 78 (45.3%;all benzathine penicillin G [BPG]-treated) obtained a Serological response to treatment;32.6% and 22.1% of patients presented with Serological failure and serofast results,respectively.For azithromycin-treated cases,66.3% and 33.7% had Serological failure and serofast results,respectively,in contrast with 1.1% and 11.3% of BPG-treated cases.However,among the A2058G-and A2059G-negative patients,the Serological response rates between the two treatment groups were similar.In multivariate analyses,patients with lower rapid plasma reagin (RPR) titers (RPR,≤ 1 ∶ 8;odds ratio [OR],0.23;95% confidence interval [CI],0.09-0.37) or

关键词： Syphilis Azithromycin Mutation Serological response Treponema pallidum

New prevalence estimate of Torque Teno virus(TTV) infection in healthy population and patients with chronic viral hepatitis in Jiujiang, China

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Virologica Sinica 2015年第3期30卷 218-220页

作者： Jin Peng Yueyue Fang Xuesen Zhao Yihong Peng Jiujiang Women's & Children's Hospital Department of Microbiology School of Basic Medical SciencePeking University

Dear Editor,Torque Teno virus(TTV)is a nonenveloped human DNA virus that was isolated from the serum of a patient with transfusion-transmitted hepatitis with unknown etiology in 1997(Nishizawa et al.,1997).TTV is the first human virus with a single-stranded circular DNA genome to be identified,and is recently classified as the Alphatorquevirus genus of the Anelloviridae family by the International Committee on Taxonomy of Viruses(ICTV)(King et al.,2011).TTV shows very high

关键词： prevalence DNA transfusion circular classified etiology stranded transmitted comparable Serological

Novel screening test for celiac disease using peptide functionalised gold nanoparticles

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World Journal of Gastroenterology 2018年第47期24卷 5379-5390页

作者： Anantdeep Kaur Olga Shimoni Michael Wallach Institute for Biomedical Materials and Devices Faculty of Science University of Tech-nology Sydney School of Life Sciences Faculty of Science University of Technology Sydney

AIM To develop a screening test for celiac disease based on the coating of gold nanoparticles with a peptide sequence derived from gliadin, the protein that triggers celiac disease.METHODS20 nm gold nanoparticles were first coated with NeutrAvidin. A long chain Polyethylene glycol(PEG) linker containing Maleimide at the Ω-end and Biotin group at the α-end was used to ensure peptide coating to the gold nanoparticles. The maleimide group with the thiol(-SH) side chain reacted with the cysteine amino acid in the peptide sequence and the biotinylated and PEGylated peptide was added to the NeutrAvidin coated gold nanoparticles. The peptide coated gold nanoparticles were then converted into a Serological assay. We used the peptide functionalised gold nanoparticle-based assay on thirty patient serum samples in a blinded assessment and compared our results with the previously run Serologicaland pathological tests on these patients.RESULTS A stable colloidal suspension of peptide coated gold nanoparticles was obtained without any aggregation. An absorbance peak shift as well as color change was caused by the aggregation of gold nanoparticles following the addition of anti-gliadin antibody to peptide coated nanoparticles at levels associated with celiac disease. The developed assay has been shown to detect anti-gliadin antibody not only in quantitatively spiked samples but also in a small-scale study on real non-hemolytic celiac disease patient's samples.CONCLUSION The study demonstrates the potential of gold nanoparticlepeptide based approach to be adapted for developing a screening assay for celiac disease diagnosis. The assay could be a part of an exclusion based diagnostic strategy and prove particularly useful for testing high celiac disease risk populations.

关键词： Celiac disease Serological point-of-care Gold nanoparticles Diagnostic test Autoantibodies

Cats as a potential source of emerging influenza virus infections

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Virologica Sinica 2015年第3期30卷 221-223页

作者： Taisuke Horimoto Fumihiro Gen Shin Murakami Kiyoko Iwatsuki-Horimoto Kentaro Kato Masaharu Hisasue Masahiro Sakaguchi Chairul A. Nidom Yoshihiro Kawaoka Department of Veterinary Microbiology University of Tokyo Division of Virology Institute of Medical Science University of Tokyo National Research Center for Protozoan Diseases Obihiro University of Agriculture and Veterinary Medicine Department of Internal Medicine II Azabu University Department of Veterinary Microbiology I Azabu University Avian Influenza Research Center Airlangga University

Dear Editor,Historically,the influenza virus has not been regarded as a major pathogen of cats.However,since 2003,natural infections of domestic cats with highly pathogenic H5N1 avian virus causing fatal cases have been reported(Songserm et al.,2006;Yingst et al.,2006;Klopfleisch et al.,2007).Furthermore,infections of this animal with A(H1N1)pdm09 virus,causing respiratory illness with some fatal cases,have also been reported in various

关键词： pathogenic avian fatal illness causing seasonal Serological emerging chicken clade

Serologic and molecular survey for major viral pathogens in grazing hybrid wild boars in Northeast China

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Journal of Integrative Agriculture 2019年第9期18卷 2133-2140页

作者： GUO Huan-cheng REN Zhao-wen DING Mei-ming XIAO Wan-jun PENG Peng HE Biao FENG Ye LIU Yan LI Xing-yu CAI Jian-qiu ZHANG Bi-kai LUO Qing-hua TU Chang-chun Institute of Military Veterinary Medicine Academy of Military Medical SciencesChangchun 130122P.R.China Division of Wildlife Conservation and Nature Reserve Management Greater Xing’an Mountains Forestry Group CorporationJiagedaqi 165000P.R.China Division of Wildlife Conservation and Nature Reserve Management Jilin Forestry AdministrationChangchun 130022P.R.China State Forestry Administration Shenyang 110034P.R.China Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose Yangzhou UniversityYangzhou 225009P.R.China

Hybrid wild boar husbandry is an important component of livestock production in Northeast China.However,the current disease situation of these animals is largely unknown due to a lack of disease surveillance.The present study was conducted to determine the prevalence of several important viral diseases in the hybrid wild boar population of Northeast China.Between September 2015 to December 2016,169 blood and 61 tissue samples were collected from apparently healthy hybrid wild boars from farms in Jilin,Inner Mongolia and Heilongjiang provinces.ELISA detected serum antibodies against classical swine fever virus(CSFV),porcine reproductive and respiratory syndrome virus(PRRSV),pseudorabies virus(PRV),porcine circovirus type 2(PCV2)and Japanese encephalitis virus(JEV),but not against African swine fever virus(ASFV),with PCV2 having the highest seropositive rate(87.2–100%in different farms).RT-PCR or PCR performed on the processed samples detected only PCV2,with 33.1%(56/169)of blood samples and 32.8%(20/61)of spleen samples being positive,respectively,indicating widespread PCV2 infection in hybrid wild boars.Phylogenetic analysis of 15 PCV2 ORF2 sequences showed that they belong to genotypes PCV2a,PCV2b and PCV2d,with nucleotide and deduced amino acid homologies of 88.5–100%and 88.1–100%,respectively.

关键词： disease transmission hybrid wild boars Northeast China Serological and molecular survey viral diseases