STUDIES ON SPECIFIC SEROLOGICAL ANTIGENS IN METACERCARIAE AND JUVENILES OF Paragoniums westermani AND ITS MONOCLONAL ANTIBODIES

作     者：蒋作君 沈一平 赵慰先 

作者机构：Nanjing Medical College Nanking 210005 PRC 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：1990年第33卷第2期

页      面：178-187页

核心收录：

学科分类：07[理学] 0703[理学-化学] 

基　　金：Project supported by the National Natural Science Foundation of China 

主　　题：paragonimiasis serological antigens monoclonal antibodies diagnosis. 

摘      要：The polyclonal antibodies to juveniles of Paragoniums westermani (PwJ-PcAbs) from sera of Wistar rats infected with Paragoniums westerrnani (P. w.)were purified by Sephadex G 200 chromatography. Next the shared serological antigens of P. w. metacercaria and juveniles (PwMJ-SAg) from the crude antigens of the metacercariae (M-NS-Ag) were purified with immuno-affinity chromatography on cyanogen bromide-activated cross-linked Sepharose 4B beads coupled withPwJ-PcAbs. PwMJ-SAg, agroup of glycoprotein molecules shown by the staining test, were specific serological antigens of P. w. metacercariae and juveniles, identified by the immunoabsorb test and immunoelectrophoresis. By SDSPAGE, PwMJ-SAg were fractionated to seven bands, including major bands A(***) and Bi(19.5 K), the two major serological antigen molecules. 20 sera samples from the patients with the nonpulmonary type of P. w. paragonimiasis were detected using PwMJ-SAg and M-NS-Ag by Dot-ELISA, and the difference of sensitivity between two antigens was highly statistically significant (P0.001). BALB/c mice, in the early stage of infection with P. w. metacercaria, were immunized with PwMJ-SAg. The spleen cells of the mice were isolated and fused with SP_2/o, a murine myeloma cell line. After three subclonal cultures, eight cell lines secreting monoclonal antibodies (McAbs) to PwMJ-SAg were prepared from 384 wells of hybridoma cells. All McAbs were IgG_1 subclass.