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Prevalence of filarial parasites in domestic and stray cats in Selangor State, Malaysia

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Asian Pacific Journal of Tropical Medicine 2015年第9期8卷 693-697页

作者： Nazeh M Al-Abd Zurainee Mohamed Nor Mustafa Kassim Marzida Mansor Abdulelah H.Al-Adhroey Romano Ngui Sinnadurai Sivanandam Department of Parasitology Faculty of Medicine University of Malaya Department of Anesthesiology Faculty of Medicine University of Malaya

Objective: To determine the prevalence of the filarial parasites, ie., Brugia malayi, Brugia pahangi(B. pahangi), Dirofilaria immitis and Dirofilaria repens(D. repens)in domestic and stray cats. Methods: A total of 170 blood sample were collected from domestic and stray cats and examined for i larial worm parasites in two localities, Pulau Carey and Bukit Gasing, Selangor State, Malaysia. Results: The overall prevalence of infection was 23.5%(40/170; 95% CI=17.4–30.6). Of this, 35%(14/40; 95% CI=22.1–50.5) and 50%(20/40; 95% CI=35.2–64.8) were positive for single B. pahangi and D. repens, respectively. The remaining of 15%(6/40; 95% CI=7.1–29.1) were positive for mixed B. pahangi and D. repens. In addition, 75% of the infected cats were domestic, and 25% were strays. No Brugia malayi and Dirofilaria immitis was detected. Eighty-four cats were captured at Pulau Carey, of which 35.7%(30/84) were infected. Among the cats determined to be infected, 93%(28/30; 95% CI=78.7–98.2) were domestic, and only 6.7%(2/30; 95% CI=19.0–21.3) were strays. Conversely, the number of infected cats was three times lower in Bukit Gasing than in Pulau Carey, and most of the cats were stray. Conclusions: B. pahangi and D. repens could be the major parasites underlying i lariasis in the study area. Adequate prophylactic plans should be administrated in the cat population in study area.

关键词： Filarial parasites Brugia Wuchereria Dirofilaria Cat Filariasis

High resolution melting real-time PCR detect and identify filarial parasites in domestic cats

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Asian Pacific Journal of Tropical Medicine 2018年第12期11卷 682-687页

作者： Darawan Nonsaithong Supit Yotmek Somsri Yotmek Hathai Nochote Sirichit Wongkamcha Sittiruk Roytrakul Usa Lek-Uthai Master of Science (Public Health) Program in Infectious Diseases and Epidemiology Faculty of Graduate Studies Mahidol University Department of Parasitology and Entomology Faculty of Public Health Mahidol University Center for Disease Control Vector Borne Disease Control Center 11.3 Surat Thani Province Department of Parasitology Faculty of Medicine Siriraj Hospital Mahidol University National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA)

Objective: To detect and identify filarial parasites in dried blood spots(DBS) collected from domestic cats using high resolution melting real-time PCR(HRM RT-PCR). Methods: A total of 208 DBS were collected from domestic cats in a brugian filariasis endemic areas in Surat Thani Province, southern Thailand. Microfilariae were found in 9 blood slides using Giemsa-stained thick blood film. The extracted DNA from blood spot volumes of 10 and 20 μL DBS with positive filarial parasites in cats were performed using HRM RT-PCR method. The primers were designed based on the partial mitochondrial 12S rRNA gene for identifying Brugia malayi, Brugia pahangi, Dirofilaria immitis. All purified samples were then detected. Results: Using different volumes of 10 μL and 20 μL DBS could easily distinguish filarial parasites and showed similar results. PCR amplicons of Brugia malayi, Brugia pahangi and Dirofilaria immitis were determined at melting peak(temperature) of 75.70℃, 77.46 ℃, and 73.56 ℃, respectively. All 9 positive DBS samples showed positive Brugia pahangi and similar nucleotide sequences. Conclusions: This HRM RT-PCR method is able to diagnose, identify and discriminate filarial parasites collected from DBS, which is simple and inexpensive compared with other probe-based genotyping methods. Furthermore, this method is useful to survey, prevent and control filariasis.

关键词： High resolution melting analysis Dried blood spot Brugia malayi Brugia pahangi Dirofilaria immitis

Serological investigation of vector-borne disease in dogs from rural areas of China

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Asian Pacific Journal of Tropical Biomedicine 2012年第2期2卷 102-103页

作者： Shiwen Wang Jing He Lijuan Zhang National Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Department of Infectious Diseases 302 Hospital of PLA

Objective:To evaluate the Anaplasma phagocytophilum(A.phagocytophilum),Ehrlichia canis(E.canis,Dirofilaria immitis(D.immitis)(canine heartworm),Borrelia burgdorferi(B.burgdorferi)infections in countryside dogs from Yunnan,Hainan and Anhui provinces.Methods:Serum samples were collected from 26 dogs in Yunnan.Hainan and Anhui provinces.The samples were tested using a commercial ELISA rapid diagnostic assay kit(SNAP^(?)4Dx^(?);IDEXX Laboratories,Inc.U.S.A.).Meaiiwliile,indirect immunofluorescence assay(IFA)recommended by WHO was conducted to delect IgG to A.phagocytophilum.Two methods were analyzed and compared.Results:The number of serologically positive dogs for IgG to A.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs responded positive for E.canu.D.immitis(canine heartworm,and B.burgdorferi by ELISA rapid diagnostic method.The number of serologically positive dogs for IgG to A.phagocytophilum was 13(50%)by IFA method.Data of the two methods were analyzed by statistical software and the difference was statistically significant(P=0.002).Conclusions:It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method.However,we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including.***.phagocytophilum,E.canis,D.immitis(canine heartworm),and B.burgdorferi which have public health significance.

关键词： Anaplasma phagocytophilum Ehrlichia canis Borrelia burgdorferi Dirofilaria immitis Dogs China Vector-borne disease Serological investigation ELISA rapid diagnostic assay

Prevalence of Canine Heartworm Disease in Stray Dogs of Grenada, West Indies

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Open Journal of Veterinary Medicine 2017年第11期7卷 168-174页

作者： Melissa C. Brown Alfred Chikweto Keshaw P. Tiwari Claude DeAllie Muhammad Iqbal Bhaiyat Ravindra N. Sharma Department of Pathobiology School of Veterinary Medicine St. George’s University Grenada West Indies

Dirofilariasis (heartworm disease) has been reported in Grenadian dogs. The aim of this cross-sectional study was to determine the prevalence of Dirofilaria immitis in stray dogs (n = 523) from the six Parishes of Grenada. Necropsy records were reviewed;information on the distribution and lesions of Dirofilaria immitis was recorded. A total of 144 from 523 dogs (27.5%;95% CI: 23.9% to 31.5%) were positive for Dirofilaria immitis on necropsy. This study shows that heartworm disease is prevalent in stray dogs in all the Parishes of mainland Grenada. This is a progressive, life-threatening disease and thus, there is a need to screen and prevent it within the stray dog population of Grenada.

关键词： Dirofilaria immitis Prevalence Stray Dogs Necropsy Grenada