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Investigation of anaplasmosis in Yiyuan County,Shandong Province,China

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Asian Pacific Journal of Tropical Medicine 2011年第7期4卷 568-572页

作者： Lijuan Zhang Feng Cui Lingling Wang Lingling Zhang Jingshan Zhang Shiwen Wang Shuxia Yang Department of Rickettsiology and Anaplasmosis National Institute of Communicable Disease Control and PreventionChina CDC Zibo Center for Disease Control & Prevention

Objective:To investigate the situation of anaplasmosis in Yiyuan county.Shandong Province. Methods:A total of 26 blood samples from febrile patients suspected of anaplasmosis,48 blood samples from healthy farmers,8 from dogs,and 10 from goats and 170 ticks were collected in the same area during 2005-2007,and detected by serological and molecular methods.Results: Eight confirmed cases and 6 probable cases were determined using serologic and molecular methods.The seroprevalence of Anaplasma phagocytophilum(A.phagocytophilum) was 26.7% in healthy cases.Nine out of 10 sheep samples and 7 out of 8 dog samples reacted positively to the A.phagocytophilum antigen.PCR amplification and sequencing of the 16SrRNA of,4. phagocytophilum gene showed that some samples from patients,goats and ticks were 100% identical.The seroprevalence of Rickettsia typhi was 22.9%,Orientia tsutsugamushi 6.3%, Rickettsia sibirica 27.1%,Coxiella burnetii 18.8%,Bartonella henselae 31.3%,and Borrelia burgdorferi 41.6%.Conclusions:It is important to make differential diagnosis of febrile patients and to apply treatment with specific antibiotics.It is needed to enforce essential prevention and control measures including tick control and to improve sanitation conditions.

关键词： Anaplasma phagocytophilum Field investigation Rickettsia Prevalence Yiyuan County Shandong Province China

Preliminary study of natural reservoirs as sentinels of <i>Anaplasma phagocytophilum</i>and <i>Ehrlichia chaffeensis</i>in Soria, northern Spain

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Open Journal of Preventive Medicine 2013年第9期3卷 501-503页

作者： Lourdes Lledó Consuelo Giménez-Pardo José Luis Serrano Consejería de Sanidad y Bienestar Social de la Junta de Castilla y León Soria Spain Departamento de Biomedicina y Biotecnología Universidad Alcalá Alcalá de Henares Spain

The serum of foxes and red deer from the Province of Soria (northern Spain) was screened in indirect immunofluorescence assays to determine whether these animals could be used as sentinels of the tick-borne pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis. The results suggest that foxes and red deer would not make good sentinels in epidemiological studies on E. chaffeensis in this region, although red deer could be used as such for the study of A. phagocytophilum transmission.

关键词： Anaplasma phagocytophilum Ehrlichia chaffeensis Foxes Red Deer Sentinels Spain

Serological investigation of vector-borne disease in dogs from rural areas of China

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Asian Pacific Journal of Tropical Biomedicine 2012年第2期2卷 102-103页

作者： Shiwen Wang Jing He Lijuan Zhang National Institute of Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Department of Infectious Diseases 302 Hospital of PLA

Objective:To evaluate the Anaplasma phagocytophilum(A.phagocytophilum),Ehrlichia canis(E.canis,Dirofilaria immitis(D.immitis)(canine heartworm),Borrelia burgdorferi(B.burgdorferi)infections in countryside dogs from Yunnan,Hainan and Anhui provinces.Methods:Serum samples were collected from 26 dogs in Yunnan.Hainan and Anhui provinces.The samples were tested using a commercial ELISA rapid diagnostic assay kit(SNAP^(?)4Dx^(?);IDEXX Laboratories,Inc.U.S.A.).Meaiiwliile,indirect immunofluorescence assay(IFA)recommended by WHO was conducted to delect IgG to A.phagocytophilum.Two methods were analyzed and compared.Results:The number of serologically positive dogs for IgG to A.phagocytophilum was only 2which was from Hainan province and none of the 26 dogs responded positive for E.canu.D.immitis(canine heartworm,and B.burgdorferi by ELISA rapid diagnostic method.The number of serologically positive dogs for IgG to A.phagocytophilum was 13(50%)by IFA method.Data of the two methods were analyzed by statistical software and the difference was statistically significant(P=0.002).Conclusions:It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method.However,we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including.***.phagocytophilum,E.canis,D.immitis(canine heartworm),and B.burgdorferi which have public health significance.

关键词： Anaplasma phagocytophilum Ehrlichia canis Borrelia burgdorferi Dirofilaria immitis Dogs China Vector-borne disease Serological investigation ELISA rapid diagnostic assay

Tick-Borne Rickettsial Pathogens in Rodents from Mexico

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Journal of Biomedical Science and Engineering 2014年第11期7卷 884-889页

作者： Carolina G. Sosa-Gutiérrez Margarita Vargas Javier Torres Guadalupe Gordillo-Pérez Facultad de Agrobiología Universidad Michoacana de San Nicolas de Hidalgo Uruapan México Unidad de Investigación Médica de Enfermedades Infecciosas y Parasitarias Hospital de Pediatría Centro Médico Nacional Siglo XXI Instituto Mexicano del Seguro Social Mexico City Mexico

Tick-Borne Rickettsial Diseases (TBRD) are emerging zoonotic diseases, and a problem of human health and veterinary medication. The distribution of these diseases is related to the distribution of vector. The presence of pathogens in the host is a risk indicator of population exposure to these areas. A total of 478 tissues samples from rodents, A. phagocytophilum 18 (3.7%), E. canis 47 (9.8%), Rickettsia rickettsii 18 (3.7%) and E. chaffeensis 19 (3.9%) were detected using species-specific PCR assay. It is the first report in Mexico the presence of rodents infected with A. phagocytophilum and E. chaffeensis. The rodent Peromyscus spp. were the most commonly prevalent host of infection for all the bacteria’s. We have to consider as host of TBRD transmitter and provide a useful contribution to understanding their epidemiology. The health sector should be considered all the fevers of unknown causes in humans and animals in Mexico as infections by these vector-borne rickettsial pathogens.

关键词： Anaplasma phagocytophilum Ehrlichia chaffeensis Rickettsia rickettsii Ehrlichia canis Rodents

日本东海静冈地区无形体病原菌的p44主要外膜蛋白基因表达区域构造解析

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内蒙古师范大学学报（自然科学汉文版） 2012年第5期41卷 518-523,530页

作者：乌日图中央民族大学中国少数民族传统医学研究院国家民委-教育部重点实验室北京100081

对日本东海地区无形体病原菌(A.phagocytophilum)的媒介蜱虫(金沟硬蜱和卵形硬蜱)的唾液腺DNA进行提取,应用分子生物学技术在亚洲地区成功地解读了p44主要外膜蛋白2.87kb的基因序列,并与欧美地区A.phagocytophilum病原体的p44主要外膜... 详细信息

对日本东海地区无形体病原菌(A.phagocytophilum)的媒介蜱虫(金沟硬蜱和卵形硬蜱)的唾液腺DNA进行提取,应用分子生物学技术在亚洲地区成功地解读了p44主要外膜蛋白2.87kb的基因序列,并与欧美地区A.phagocytophilum病原体的p44主要外膜蛋白基因进行序列比对,确定了p44主要外膜蛋白表达基因在欧美以及亚洲地域间的差异性.

关键词： Anaplasma phagocytophilum Humanan aplasmosis p44主要外膜蛋白 p44 multigene family 无形体病原菌媒介蜱虫