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Chinese Journal of Natural Medicines 2017年第4期15卷 271-280页

作者： Saba Khanam Ambreen Fatima Rahul Smita Jyoti Fahad Ali Falaq Naz Barkha Shakya Yasir Hasan Siddique Drosophila transgenic Laboratory Section of GeneticsDepartment of ZoologyFaculty of Life SciencesAligarh Muslim UniversityAligarhUttar Pradesh 202002India

Capsaicin(trans-8-methyl-N-vanillyl-6-nonenamide) is the main component in hot peppers, including red chili peppers, alapenos, and habanero, belonging to the genus Capsicum. Capsaicin is a potent antioxidant that interferes with free radical activities. In the present study, the possible protective effect of capsaicin was studied against methyl methanesulphonate(MMS) induced toxicity in third instar larvae of transgenic Drosophila melanogaster(hsp70-lacZ)Bg^9. The third instar was allowed to feed on the diet having different doses of capsaicin and MMS separately and in combination. The results suggested that the exposure of third instar larvae to the diet having MMS alone showed significant hsp70 expression as well as tissue DNA and oxidative damage, whereas the larvae feed on the diet having MMS and capsaicin showed a decrease in the toxic effects for 48-h of exposure. In conclusion, capsaicin showed a dose-dependent decrease in the toxic effects induced by MMS in the third instar larvae of transgenic Drosophila melanogaster.

关键词： Capsaicin Methyl methanesulphonate Drosophila melanogaster Toxicity

Role of Ocimum sanctum leaf extract on dietary supplementation in the transgenic Drosophila model of Parkinson's disease

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Chinese Journal of Natural Medicines 2014年第10期12卷 777-781页

作者： Yasir Hasan Siddique Mohammad Faisal Falaq Naz Smita Jyoti Rahul Drosophila transgenic Laboratory Section of GeneticsDepartment of ZoologyFaculty of Life SciencesAligarh Muslim UniversityAligarhUttar PradeshIndia Forest Entomology Division Forest Research InstituteDehradun

AIM: To evaluate the effect of Ocimum sanctum leaf extract on the dietary supplementation in the transgenic Drosophila model of Parkinson's disease. METHOD: The effect of Ocimum sanctum leaf extract was studied on the transgenic Drosophila model of flies expressing normal human alpha synuclein(h-αs) in the neurons. O. sanctum extract at final concentrations of 0.042 8 × 10-4, 0.87 × 10-4, and 1.85 × 10-4 g·mL-1 of diet were established and the flies were allowed to feed for 21 days. The climbing assay and lipid peroxidation were taken as parameters for the study. RESULTS: The supplementation of O. sanctum extract showed a dose-dependent significant delay in the loss of climbing ability and reduction in oxidative stress in the brain of PD model flies. CONCLUSION: The results of the present study showed that the O. sanctum extract is potent in reducing the PD symptoms in transgenic Drosophila model.

关键词：帕金森疾病呈现喂食允许

LSD1调控颗粒细胞自噬并抑制Wt1参与FSH调节的有腔卵泡形成

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Science Bulletin 2024年第8期69卷 1122-1136页

作者：朱子健何美娜张拓赵庭秦韶刚高萌王文基郑文影陈子琦刘龙萍郝明周波张华王建斌王凤超夏国良王超 State Key Laboratory of Farm Animal Biotech Breeding College of Biological SciencesChina Agricultural UniversityBeijing 100193China College of Basic Medicine Guizhou Medical UniversityGuiyang 550025China Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases Department of PhysiologyCollege of Basic MedicineGuizhou Medical UniversityGuiyang 550025China School of Life Sciences Taizhou UniversityTaizhou 318000China School of Life Sciences Tsinghua UniversityBeijing 100084China transgenic Animal Center National Institute of Biological SciencesBeijing 102206China Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China College of Life ScienceNingxia UniversityYinchuan 750021China

In a growing follicle,the survival and maturation of the oocyte largely depend on support from somatic cells to facilitate FSH-induced mutual signaling and chemical communication.Although apoptosis and autophagy in somatic cells are involved in the process of FSH-induced follicular development,the underlying mechanisms require substantial study.According to our study,along with FSH-induced antral follicles(AFs)formation,both lysine-specific demethylase 1(LSD1)protein levels and autophagy increased simultaneously in granulosa cells(GCs)in a time-dependent manner,we therefore evaluated the importance of LSD upon facilitating the formation of AFs correlated to autophagy in GCs.Conditional knockout of Lsdl in GCs resulted in significantly decreased AF number and subfertility in females,accompanied by marked suppression of the autophagy in GCs.On the one hand,depletion of Lsd1 resulted in accumulation of Wilms tumor 1 homolog(WT1),at both the protein and mRNA levels.WT1 prevented the expression of FSH receptor(Fshr)in GCs and thus reduced the responsiveness of the secondary follicles to FSH induction.On the other hand,depletion of LSD1 resulted in suppressed level of autophagy by upregulation of ATG16L2 in GCs.We finally approved that LSD1 contributed to these sequential activities in GCs through its H3K4me2 demethylase activity.Therefore,the importance of LSD1 in GCs is attributable to its roles in both accelerating autophagy and suppressing WT1 expression to ensure the responsiveness of GCs to FSH during AFs formation.

关键词： LSD1 Autophagy H3K4me2 WT1 Follicle formation Follicle stimulating hormone

Expression of the Japanese oak silkworm Antheraea yamamai fibroin gene in the domesticated silkworm Bombyx mori

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Insect Science 2009年第6期16卷 465-473页

作者： Isao Kobayashi Katsura Kojima Hideki Sezutsu Keiro Uchino Toshiki Tamura transgenic Silkworm Research Center Silk Materials Research Unit National Institute of Agrobiological Sciences Ibaraki Japan

To understand the evolutionary conservation ofthe gene expression mechanism and secretion machinery between Antheraea and Bombyx fibroins, we introduced the genomic A. yamamai fibroin gene into the domesticated silkworm, B. mori. The spliced A. yamamai fibroin mRNA appeared only in the posterior region of the silk gland of the transgenic silkworm, suggesting that the functions of the fibroin promoter region and the splicing machinery are conserved between these two species. The A. yamarnai fibroin protein was detected in the lumen of the silk gland of the transgenic silkworm, albeit at lower levels compared with the B. mori-type fibroin. We found a strong degeneration of the posterior region of the silk gland of the transgenic silkworm. As a result, the cocoon shell weight was much lower in the transgenic silkworm than in the non-transgenic line. These results indicate that the promoter function and splicing machinery are well conserved between A. yamamai and B. mori but that the secretion mechanism of fibroin is diversified between the two.

关键词： Antheraea yamamai Bombyx mori evolution fibroin silk transgenic

Efficient Differentiation of Embryonic Stem Cells into Neurons in Glial Cell-conditioned Medium under Attaching Conditions

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 480-487页

作者： Hai-Bin TIAN~(1,2) Zeng-Liang BAI~1 Hong WANG~2 Jian-Quan CHEN~2 Guo-Xiang CHENG~2 1 Institute of Development Biology,School of Life Science,Shandong University,Jinan 250100,China 2 Shanghai transgenic Research Center,Shanghai 201203,China Institute of Development Biology School of Life Science Shandong UniversityJinan 250100 China Shanghai transgenic Research CenterShanghai 201203 China

Embryonic stem (ES) cells can differentiate into neurons in vitro,which provides hope for thetreatment of some neurodegenerative diseases through cell transplantation.However,it remains a challengeto efficiently induce ES cells to differentiate into neurons.Here,we show that murine ES cells can efficientlydifferentiate into neurons when cultured in glial cell-conditioned medium (GCM) under attaching conditionswithout the formation of embryoid bodies.In comparison with murine embryonic fibroblast-conditionedmedium,we found that GCM has a positive effect on limiting the generation of non-neuronal cells,such asastrocytes.In addition,compared with suspension conditions,attaching conditions delay the differentiationprocess of ES cells.

关键词： embryonic stem cell glial cell-conditioned medium differentiation neuron attaching conditions

Impacts of prenatal nutrition on animal production and performance： a focus on growth and metabolic and endocrine function in sheep

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Journal of Animal Science and Biotechnology 2018年第1期9卷 10-23页

作者： Prabhat Khanal Mette Olaf Nielsen Department of Veterinary and Animal Sciences Faculty of Health and Medical SciencesUniversity of Copenhagen Gronnegardsvey 31st floorDK-1870 Frederiksberg CDenmark Department of Nutrition Faculty of MedicineTransgenic Animal and Lipid StorageNorwegian Transgenic Centre(NTS)University of OsloOsloNotway

The concept of foetal programming（FP） originated from human epidemiological studies, where foetal life nutrition was linked to health and disease status later in life. Since the proposal of this phenomenon, it has been evaluated in various animal models to gain further insights into the mechanisms underlying the foetal origins of health and disease in humans. In FP research, the sheep has been quite extensively used as a model for humans. In this paper we will review findings mainly from our Copenhagen sheep model, on the implications of late gestation malnutrition for growth, development, and metabolic and endocrine functions later in life, and discuss how these implications may depend on the diet fed to the animal in early postnatal life. Our results have indicated that negative implications of foetal malnutrition, both as a result of overnutrition and, particularly, late gestation undernutrition, can impair a wide range of endocrine functions regulating growth and presumably also reproductive traits. These implications are not readily observable early in postnatal life, but are increasingly manifested as the animal approaches adulthood. No intervention or cure is known that can reverse this programming in postnatal life. Our findings suggest that close to normal growth and slaughter results can be obtained at least until puberty in animals which have undergone adverse programming in foetal life, but manifestation of programming effects becomes increasingly evident in adult animals.Due to the risk of transfer of the adverse programming effects to future generations, it is therefore recommended that animals that are suspected to have undergone adverse FP are not used for reproduction. Unfortunately, no reliable biomarkers have as yet been identified that allow accurate identification of adversely programmed offspring at birth,except for very low or high birth weights, and, in pigs, characteristic changes in head shape（dolphin head）. Future efforts should be therefore dedicated to identify reliable biomarkers and

关键词： Adipose tissue Endocrine function Foetal programming Metabolic function Sheep

See the color,see the seed:GmW1 as a visual reporter for transgene and genome editing in soybean

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The Crop Journal 2023年第1期11卷 311-315页

作者： Li Chen Shan Yuan Yupeng Cai Weiwei Yao Qiang Su Yingying Chen Jialing Zhang Wensheng Hou National Center for transgenic Research in Plants Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

A fast and efficient recognition method of transgenic lines will greatly improve detection efficiency and reduce cost.In this study,we successfully identified the transgenic soybean plants by the color.We isolated a GmW1 gene encoding a flavonoid 3'5'-hydroxylase from a soybean cultivar ZH42(purple flower).We found that purple flowers occurred in the overexpression lines in the Jack and Williams 82 backgrounds(white flower).All plants with purple flowers were positive,and this trait seems stably inherited in the offspring.We have also obtained the editing plants,which were classified into three types according to the different flower colors appeared.We analyzed the phenotype and the homozygous types of the T_1mutants.We also found that a correspondence between flower color and stem color.This study provides a visible color reporter on soybean transformation.It can be quickly and early to identify the transgenic soybean plants by stem color of seedlings,which substantially reduces the amount of labor and cost.

关键词： Soybean GmW1 Color transgenic lines Genome editing

Optimisation of Adventitious Shoot Regeneration and Agrobacterium-mediated Transformation in Canna × generalis(Canna Lily)

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Horticultural Plant Journal 2019年第1期5卷 39-46页

作者： Rani Singh Arvind Kumar Dubey Indraneel Sanyal Plant transgenic Laboratory Division of Molecular Biology and Biotechnology CSIR-National Botanical Research Institute

Canna being ornamental plants has a significant role in agriculture, medical, economy and food industry. Canna has a limited vase life due to the rapid loss of moisture from its perianth. For improving its market value, cuticularisation of the perianth can be achieved by the expression of a heterologous cutin producing gene, using the tissue culture and transformation protocol developed in this study. Efficient, rapid and direct adventitious shoot regeneration was successfully established in Canna × generalis using recalcitrant rhizome explants. The explants were cultured on MS medium supplemented with 6-benzylaminopurine(6-BA), thidiazuron(TDZ), and kinetin. Among the four genotypes taken for tissue culture, the ‘Trinacria variegata' was the best responding cultivar. And 2 mg · L^(-1)6-BA or 1.5 mg · L^(-1) TDZ along with 0.1 mg · L^(-1) IAA was optimum for their regeneration. The highest regeneration was achieved in ‘Trinacria variegata'(36%) on 6-BA, 33% on TDZ while kinetin failed to evoke any regenerative responses. Regeneration was enhanced by supplementation of 100 mg · L^(-1) ascorbic acid(AsA), while, 100 mg · L^(-1) of l-cysteine or 100 mg · L^(-1) dithiothreitol(DTT), inhibited regeneration. Shoots were observed to develop 3–5 fibrous roots on MS medium supplemented with 0.5 mg · L^(-1) indole-3-butyric acid(IBA). The plantlets were transplanted into pots and acclimatised in glasshouse with 100%survival. For transformation of Canna, rhizome explants were co-cultivated for 60 min in Agrobacterium suspension. The explants were washed with 500 mg · L^(-1) cefotaxime solution, subjected to 100 mg · L^(-1) kanamycin selection followed by excision of the shoots and culturing them on IBA-supplemented media for root development. Transgene integration in the putative transformants was confirmed by PCR assay and copy number by Southern blot hybridisation analysis.

关键词： Canna regeneration rhizome UidA Agrobacterium transformation ascorbic acid 6-BA TDZ

Gene expression analysis in the larval silk gland of the eri silkworm Samia ricini

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Insect Science 2016年第6期23卷 791-804页

作者： Takuya Tsubota Kimiko Yamamoto Kazuei Mita Hideki Sezutsu transgenic Silkworm Research Unit Ibaraki 305-8634Japan Insect Genome Research Unit National Institute of Agrobiological Sciences Ibaraki 305-8634Japan

Insects produce silk for a range of purposes. In the Lepidoptera, silk is utilized as a material for cocoon production and serves to protect larvae from adverse environmental conditions or predators. Species in the Saturniidae family produce an especially wide variety of cocoons, for example, large, golden colored cocoons and those with many small holes. Although gene expression in the silk gland of the domestic silkworm （Bombyx mori L.） has been extensively studied, considerably fewer investigations have focused on members of the saturniid family. Here, we established expression sequence tags from the silk gland of the eri silkworm （Samia ricini）, a saturniid species, and used these to analyze gene expression. Although we identified thefibroin heavy chain gene in the established library, genes for other major silk proteins, such asfibroin light chain andfibrohexamerin, were absent. This finding is consistent with previous reports that these latter proteins are lacking in saturniid silk. Recently, a series offibrohexamerin-like genes were identified in the Bombyx genome. We used this information to conduct a detailed analysis of the library established here. This analysis identified putative homologues of these genes. We also found several genes encoding small silk protein molecules that are also present in the silk of other Lepidoptera. Gene expression patterns were compared between eri and domestic silkworm, and both conserved and nonconserved expression patterns were identified for the tested genes. Such differential gene expression might be one of the major causes of the differences in silk properties between these species. We believe that our study can be of value as a basic catalogue for silk gland gene expression, which will yield to the further understanding of silk evolution.

关键词： domestic silkworm eri silkworm EST analysis Samia ricini silk gland

The sound of movement,a noninvasive surgical procedure for treating Parkinson’s disease

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Aging Communications 2023年第1期5卷 1-3页

作者： Lupeng Wang Huaibin Cai transgenic Section Laboratory of NeurogeneticsNational Institute on AgingNational Institutes of HealthBethesdaMD 20892U.S.A.

Parkinson’s disease(PD)is the second most common degenerative neurological disorder after Alzheimer’s disease.As one of fastest growing neurological conditions,PD affects millions of elderly people worldwide.PD patients display progressive motor symptoms,including resting tremor,slowed movement,impaired posture and balance,and rigid muscles[1].Additionally,they also often suffer from chronic pain,depression,dementia,and other non-motor symptoms[2].Medications and surgery can improve patient’s motor performance to some degree,while the treatment for non-motor conditions is limited.Moreover,long-term medication can cause severe side effects,such as dyskinesia and impulse control disorders[3,4].Therefore,new mechanistic insights and therapeutic agents/procedures are still needed to improve the treatment of increasing number of PD patients.

关键词： impaired movement treatment