Expression of the Japanese oak silkworm Antheraea yamamai fibroin gene in the domesticated silkworm Bombyx mori
Expression of the Japanese oak silkworm Antheraea yamamai fibroin gene in the domesticated silkworm Bombyx mori作者机构：Transgenic Silkworm Research Center Silk Materials Research Unit National Institute of Agrobiological Sciences Ibaraki Japan
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2009年第16卷第6期
页 面：465-473页
核心收录：
学科分类：07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 090102[农学-作物遗传育种] 0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0905[农学-畜牧学] 0904[农学-植物保护] 0901[农学-作物学] 0836[工学-生物工程] 0713[理学-生态学]
基 金：Ministry of Agriculture, Forestry, and Fisheries (MAFF) of Japan Bio-Oriented Technology Research Advancement Institution, Japan MAFF Ministry of Education, Culture, Sports, Science, and Technology, Japan
主 题：Antheraea yamamai Bombyx mori evolution fibroin silk transgenic
摘 要：To understand the evolutionary conservation ofthe gene expression mechanism and secretion machinery between Antheraea and Bombyx fibroins, we introduced the genomic A. yamamai fibroin gene into the domesticated silkworm, B. mori. The spliced A. yamamai fibroin mRNA appeared only in the posterior region of the silk gland of the transgenic silkworm, suggesting that the functions of the fibroin promoter region and the splicing machinery are conserved between these two species. The A. yamarnai fibroin protein was detected in the lumen of the silk gland of the transgenic silkworm, albeit at lower levels compared with the B. mori-type fibroin. We found a strong degeneration of the posterior region of the silk gland of the transgenic silkworm. As a result, the cocoon shell weight was much lower in the transgenic silkworm than in the non-transgenic line. These results indicate that the promoter function and splicing machinery are well conserved between A. yamamai and B. mori but that the secretion mechanism of fibroin is diversified between the two.
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