Efficient Differentiation of Embryonic Stem Cells into Neurons in Glial Cell-conditioned Medium under Attaching Conditions

作     者：Hai-Bin TIAN~(1,2) Zeng-Liang BAI~1 Hong WANG~2 Jian-Quan CHEN~2 Guo-Xiang CHENG~2 1 Institute of Development Biology,School of Life Science,Shandong University,Jinan 250100,China 2 Shanghai Transgenic Research Center,Shanghai 201203,China 

作者机构：Institute of Development Biology School of Life Science Shandong UniversityJinan 250100 China Shanghai Transgenic Research CenterShanghai 201203 China 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2005年第37卷第7期

页      面：480-487页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：This work was supported by a grant from the Shanghai Genon Bioengineering Company Limited 

主　　题：embryonic stem cell glial cell-conditioned medium differentiation neuron attaching conditions 

摘      要：Embryonic stem (ES) cells can differentiate into neurons in vitro,which provides hope for thetreatment of some neurodegenerative diseases through cell ***,it remains a challengeto efficiently induce ES cells to differentiate into ***,we show that murine ES cells can efficientlydifferentiate into neurons when cultured in glial cell-conditioned medium (GCM) under attaching conditionswithout the formation of embryoid *** comparison with murine embryonic fibroblast-conditionedmedium,we found that GCM has a positive effect on limiting the generation of non-neuronal cells,such *** addition,compared with suspension conditions,attaching conditions delay the differentiationprocess of ES cells.