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Asian Journal of Andrology 2005年第4期7卷 411-417,页

作者： Paul F.Moundipa Nathalie Sara E.Beboy Fabien Zelefack Silvère Ngouela Etienne Tsamo Wolf-Bernhard Schill Thomas K.Monsees Departments of Biochemistry Organic Chemistry University of Yaounde IP.O.Box 812 YaoundeCameroon Centre for Dermatology and Andrology Justus Liebig UniversityGaffkystr.1435385 GiessenGermany Centre for Dermatology and Andrology Justus Liebig UniversityGaffkystr.1435385 GiessenGermany Department of AnatomyUniversity of TechnologyFetscherstr.7401307 DresdenGermany

Aim： To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes. Methods： The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using ^125I-radioimmunoassay （^125I-RIA）. Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes. Results： bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin （hCG）. Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations. Conclusion： Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.

关键词： bull rats Leydig cells testosterone Basella alba Hibiscus macranthus plant extract

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DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein bull testis and are correlated with sperm quality

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BIOCELL 2021年第1期45卷 79-87页

作者： YONG LIU ZHIHUA JU QIANG JIANG WENHAO LIU CHUNHONG YANG YARAN ZHANG XIUGE WANG YAPING GAO XIAOCHAO WEI YAN SUN JINPENG WANG MINGHAI HOU LING YANG JINMING HUANG Dairy Cattle Research Center Shandong Academy of Agricultural SciencesJinan250100China College of Life Sciences and Food Engineering Hebei University of EngineeringHandan056021China

F-box and WD-40 domain protein 11(FBXW11)is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of *** our previous research,the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low *** the present study,the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance *** immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous *** splice variants of the FBXW11 gene,namely,FBXW11-tv1,FBXW11-tv2,and FBXW11-tv3,were identified in testicular *** splicing patterns of the three variants are exon *** transcript FBXW11-tv2 expressions were the highest in each *** low-performance groups displayed higher FBXW11-tv1 and FBXW11-tv2 transcript expressions than the normal performance *** CpG islands were located within the 5’UTR and exon 1-2 region of the FBXW11 *** sequencing PCR results demonstrated that the methylation levels of 11 methylation sites in the CpG island 2 from−99 to−43 in the normal performance groups were significantly lower than those in the low-performance *** correlation analysis suggested that the CpG island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression *** data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and were correlated with sperm quality traits during spermatogenesis in Holsteins.

关键词： FBXW11 Alternative splicing DNA methylation bull Sperm quality traits

Amplification and Characterization of bull Semen Infected Naturally with Foot-and-mouth Disease Virus Type Asia1 by RT-PCR

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Virologica Sinica 2008年第5期23卷 378-382页

作者： Jun-jun SHAO Hui-yun CHANG Tong LIN Guo-zheng CONG Jun-zheng DU Jian-hong GUO Hui-fang BAO You-jun SHANG Ya-min YANG Xiang-tao LIU Zai-xin LIU Ji-xing LIU Key Laboratory of Animal Virology of Minister of Agriculture State key Laboratory of Veterinary EtiologyicalBiology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046China

To investigate the security of semen biologically, 15 bull semen samples were collected (of which 5 exhibited clinical signs of Foot-and-mouth disease) and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by Foot-and-mouth disease virus (FMDV), but FMDV was not detected in semen samples from those bulls not showing clinical signs of Foot-and-mouth disease (FMD). This is the first report of the presence of FMDV in bull semen due to natural infection in China. The analysis of the partial sequence of the VP1 gene showed that the virus strain isolated from semen has 97.9% identity with the virus isolated from vesicular liquid of infected bulls showing typical signs of FMD and belonged to the same gene sub-group.

关键词： bull Foot-and-mouth disease (FMD) Foot-and-mouth disease virus (FMDV) RT-PCR Semen

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Effects of enzymatic and non-enzymatic antioxidants in diluents on cryopreserved bull epididymal sperm

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Asian pacific Journal of Reproduction 2022年第1期11卷 44-50页

作者： Hasan Sakhdary Abbas Farshad Jalal Rostamzadeh Fateme Bahri Binabaj Keyvan Sobhani Laboratory of Reproduction Biology Department of Animal SciencesFaculty of AgricultureUniversity of KurdistanSanandajKurdistanIran Department of Animal Sciences Faculty of AgricultureGonbad Kavous UniversityGonbad KavousGorganIran

Objective:To evaluate the supplementation effects of vitamin E,vitamin C,superoxide dismutase(SOD),and catalase to diluents on bull cryopreserved epididymal ***:Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E,5.0 mM vitamin C,100.0 IU/mL SOD,and 100.0μg/mL catalase alone,or in a *** control treatment contained no *** supplementation,samples were frozen and stored in liquid *** sperm parameters including motility,progressive motility,viability,acrosome integrity,plasma membrane integrity,kinematics and DNA damage were evaluated following the thawing ***:Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment(Pbull epididymal sperm and could be used for cryopreservation.

关键词： Antioxidant Cryopreservation Spermatological characteristics DNA damage bull Vitamin E Vitamin C Superoxide dismutase Catalase Oxidative stress

Polymyxin B effects on motility parameters of cryopreserved bull semen

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Asian pacific Journal of Reproduction 2017年第1期6卷 38-45页

作者： Mojtaba Rashedi Mohammad Hashem Fazeli Hamid Gholami Mohammad Bahreini Young Researchers and Elites Club Faculty of Veterinary MedicineIslamic Azad UniversityShahrekord BranchShahrekordIran Department of Clinical Science Faculty of Veterinary ScienceIslamic Azad UniversityShahrekord BranchShahrekordIran Jahed Animal Institutions Company KarajAlborz provinceIran Embryology laboratory Interbreeding CenterKarajAlborz provinceIran

Objective:To evaluate the effect of adding different values of polymyxin B (PMB) to bull semen on various motility parameters of post-thawed semen such as total motility, progressive motility and velocity parameters using kinetic parameters of sperm by Computer Assisted Sperm ***: Gram negative bacteria release lipopolysaccharide, which induces the apoptotic pathway. Antibiotics are added to semen in order to prevent bacterial contaminations in bovine semen. These antibiotics kill the bacteria especially gram negative bacteria. Therefore, their endotoxins are released during bacteriolysis and bind to the head region and midpiece of sperm. PMB is a bactericidal antibiotic against multidrug resistant gram-negative bacteria and is able to neutralize the toxic effects of the released endotoxin. This study was performed on 3-year old Taleshi bulls. Results:The results showed both positive and negative significant effects of PMB on semen quality. Total motility and progressive motility were significantly increased (Pbull semen is advised, but before using it, the rate of endotoxins must be ***: We advise using PMB after measuring endotoxin concentration;In vitro,in vivoand in field fertilization, adding other sperm evaluation factors such as acrosomal integrity, DNA integrity, mitochondrial function to PMB treated semen.

关键词： Taleshi bull semen Polymyxin B Computer Assist Semen Analyzer Motility parameters

Association of Osteopontin Gene Promoter Single Nucleotide Polymorphisms with bull Semen Quality

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Advances in Reproductive Sciences 2016年第1期4卷 1-7页

作者： Rick W. Rorie Chance L. Williams Toby D. Lester Department of Animal Science University of Arkansas Fayetteville AR USA

Osteopontin (OPN) is a protein found at higher concentrations in the seminal plasma of bulls with above average fertility. Polymorphisms have been reported within the OPN gene promoter that can affect production of this protein and thus, affect fertility. Therefore, Angus (n = 5) and Angus x Gelbvieh (Balancer, n = 14) and Angus x Brahman (n = 15) bulls were evaluated for presence of single nucleotide polymorphisms (SNP) in the Bos taurus OPN gene (GenBank: AY878328.1) promoter region, and their possible effects on bull semen quality as evaluated by computer-assisted semen analysis (CASA). Semen was collected by electroejaculation 6 to 9 times from each bull, and each semen collection was evaluated by CASA for motile, progressive and rapid sperm within 5 mins of ejaculation. The bulls were genotyped for reported single nucleotide polymorphisms (SNP) in the promoter region of the OPN gene through amplification of two 700 base pair (bp) DNA fragments and sequencing of the resulting PCR products. Seven SNP sites were identified, at bp 3379, 3490, 3492, 5075, 5205, 5209, and 5263 of the OPN gene. The SNP identified at bp 5205, 5209 and 5263 had not been previously reported. Individual SNP sites were evaluated as the main effect on CASA sperm motility variables in a SAS MIXED model for repeated measures. A thymine to guanine substitution at bp 3379 was associated with increased (P ≤ 0.02) percentage of motile, progressive and rapid sperm in Angus x Brahman bulls, and tended (P ≤ 0.10) to increase the same sperm motility parameters in Angus, and Angus x Gelbvieh bulls. The percentages of motile, progressive and rapid sperm were similar (P ≥ 0.05) among genotypes for the other 6 SNP identified. These results suggest that identification and genotyping of polymorphisms within the promoter region of the bovine OPN gene may be useful for selecting bulls with improved sperm motility parameters.

关键词： Osteopontin Polymorphism Sperm Motility bull

Studies on the quality improvement of frozen semen of bull with treatment of helium-neon laser

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Chinese Journal of Lasers 1993年第6期 87-90页

作者： Wenbin Yue, Kelu Li(Animal Science Department, Shanxi Agricultural University, Taigu, Shanxi 030801 China)Jianxin Lu (Shanxi Animal Husbandry Bureau,China)Chaohui Huo(Biological Drugs Manufacturing Plant of Veterinary Medicine, Shanxi Province,China)

Studies on the improvement of the quality of bull’s forzen semen witht reatment of He-Ne laser are presented. It is found experimently that the quality of forzen semen is greatly improved with proper irradiation dose of laser. For two treated groups with 2.7J/cm~2 and 4.5J/cm~2 doses, the unfrozen vitality, respiration, fructose utilization and ~32Pi intake are much higher (p<0. 01 and p<0.05, respectively) than those in control. The acrosome integrity rate and lifetime are also higher (p<0.05) than those in control for the group treacted with 2.7J/cm~2 dose. The mechanism on the improvement of semon quality with laser irradiation is investigated.

关键词： bull frozen semen Helium-neon laser

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RED bull RB5

汽车时代 2009年第5期 28-29页

作者：金松（日译中）

定点观测虽然脚伤后遗症还没有消除，但韦伯却像丝毫没受影响一样，和维特尔一同跑出好成绩。赛车以低负重，低车高的设置，来提高空气下压力。这款艾德里安·纽维个人风格鲜明的赛车。其流线型的设计应该会让置身其中的车手很不舒服，... 详细信息

定点观测虽然脚伤后遗症还没有消除，但韦伯却像丝毫没受影响一样，和维特尔一同跑出好成绩。赛车以低负重，低车高的设置，来提高空气下压力。这款艾德里安·纽维个人风格鲜明的赛车。其流线型的设计应该会让置身其中的车手很不舒服，但即使这样他的表现仍然很出色。第一个弯道就利用机械性抓地力成功上位，做得很漂亮。

关键词： RED bull RB5 赛车定点观测弯道

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Red bull红牛斯德哥尔摩总部办公室

现代装饰 2015年第11期 76-83页

作者： Thérèse Svalling Beata Denton 姜敏华 pS Arkitektur Jason Strong Photography

本案是红牛位于瑞典斯德哥尔摩的新总部,由瑞典pS建筑事务所设计打造,项目彰显出红牛公司对于冒险、文化、体育、音乐、艺术充满无限热情的企业精神。整个空间以蓝色、黑色、金色为主基调,设计师利用丰富的自然材料如大理石、皮革、木... 详细信息

本案是红牛位于瑞典斯德哥尔摩的新总部,由瑞典pS建筑事务所设计打造,项目彰显出红牛公司对于冒险、文化、体育、音乐、艺术充满无限热情的企业精神。整个空间以蓝色、黑色、金色为主基调,设计师利用丰富的自然材料如大理石、皮革、木材等进行调色搭配,营造出积极又具冒险精神的空间氛围。

关键词： Red bull 企业精神建筑事务所空间氛围冒险精神建筑元素人体工程学私人空间休息场所丝网印刷

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增强创新:METRIX^(TM) bull技术在造纸中的应用

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中华纸业 2015年第18期36卷 41-44页

作者：徐娜肖金海黄凯宏朱博赵玉林张猛艺康(中国)投资有限公司上海200062

METRIX bull技术是纳尔科公司(艺康集团子公司)近期推出的纸张增强创新方案,可以全方位提高成纸强度、成纸灰分、灰分保留及网部/压榨部脱水。在帮助造纸商实现原料成本节降、化学品方案优化、高强度纸种开发、纸机效率提升以及能源节... 详细信息

METRIX bull技术是纳尔科公司(艺康集团子公司)近期推出的纸张增强创新方案,可以全方位提高成纸强度、成纸灰分、灰分保留及网部/压榨部脱水。在帮助造纸商实现原料成本节降、化学品方案优化、高强度纸种开发、纸机效率提升以及能源节省等方面具有广泛的应用前景。本文从实验室和现场纸机试验中对METRIX bull技术进行了全面的研究。实验室结果表明:METRIX bull技术在纸张内聚力、湿强度、成纸灰分与首程留着率方面都表现出比市场常用干强剂更为优异的性能;现场纸机试验结果表明:METRIX bull技术可以提高约20%成纸层间结合强度,提高成纸灰分3~5个百分点,显著地降低生产成本,取得了客户满意的试验结果。

关键词： METRIX bull 成纸强度成纸灰分增强剂

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