Amplification and Characterization of Bull Semen Infected Naturally with Foot-and-mouth Disease Virus Type Asia1 by RT-PCR

作     者：Jun-jun SHAO Hui-yun CHANG Tong LIN Guo-zheng CONG Jun-zheng DU Jian-hong GUO Hui-fang BAO You-jun SHANG Ya-min YANG Xiang-tao LIU Zai-xin LIU Ji-xing LIU 

作者机构：Key Laboratory of Animal Virology of Minister of Agriculture State key Laboratory of Veterinary EtiologyicalBiology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2008年第23卷第5期

页      面：378-382页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：State Science and Technology Support Program (2006DAD06A03) Hi-tech Research and Development Program of China 863 (2006AA10A204) 

主　　题：Bull Foot-and-mouth disease (FMD) Foot-and-mouth disease virus (FMDV) RT-PCR Semen 

摘      要：To investigate the security of semen biologically, 15 bull semen samples were collected (of which 5 exhibited clinical signs of Foot-and-mouth disease) and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by Foot-and-mouth disease virus (FMDV), but FMDV was not detected in semen samples from those bulls not showing clinical signs of Foot-and-mouth disease (FMD). This is the first report of the presence of FMDV in bull semen due to natural infection in China. The analysis of the partial sequence of the VP1 gene showed that the virus strain isolated from semen has 97.9% identity with the virus isolated from vesicular liquid of infected bulls showing typical signs of FMD and belonged to the same gene sub-group.