检索结果-南通市图书馆

Sperm chromatin condensation as an in vivo fertility biomarker in Bulls:a flow cytometry approach

Journal of Animal Science and Biotechnology 2022年第2期13卷 418-429页

作者： Marc Llavanera Jordi Ribas-Maynou Ariadna Delgado-Bermúdez Sandra Recuero Rodrigo Muiño Carlos OHidalgo Carolina Tamargo Sergi Bonet Yentel Mateo-Otero Marc Yeste Biotechnology of Animal and Human Reproduction(TechnoSperm) Faculty of SciencesUniversity of GironaC/Maria Aurèlia Campany69ES-17003 GironaSpain Department of Biology Unit of Cell BiologyFaculty of SciencesUniversity of GironaES-17003 GironaSpain Department of Animal Pathology Faculty of Veterinary MedicineUniversity of Santiago de CompostelaES-15705 LugoSpain Department of Animal Selection and Reproduction The Regional Agri-Food Research and Development Service of Asturias(SERIDA)E-33394 GijónSpain

Background:Genetic selection in cattle has been directed to increase milk production.This,coupled to the fact that the vast majority of bovine artificial inseminations(AI)are performed using cryopreserved sperm,have led to a reduction of fertility rates over the years.Thus,seeking sensitive and specific sperm biomarkers able to predict fertility rates is of vital importance to improve cattle reproductive efficiency.In humans,sperm chromatin condensation evaluated through chromomycin A3(CMA3)has recently been purported to be a powerful biomarker for sperm functional status and male infertility.The objectives of the present study were:a)to set up a flow cytometry method for simultaneously evaluating chromatin condensation and sperm viability,and b)to test whether this parameter could be used as a predictor of in vivo fertility in Bulls.The study included pools of three independent cryopreserved ejaculates per Bull from 25 Holstein males.Reproductive outcomes of each sire were determined by non-return rates,which were used to classify Bulls into two groups(highly fertile and subfertile).Results:Chromatin condensation status of bovine sperm was evaluated through the combination of CMA3 and Yo-Pro-1 staining and flow cytometry.Sperm quality parameters(morphology,viability,total and progressive motility)were also assessed.Pearson correlation coefficients and ROC curves were calculated to assess their capacity to predict in vivo fertility.Sperm morphology,viability and total motility presented an area under the ROC curve(AUC)of 0.54,0.64 and 0.68,respectively(P>0.05),and thus were not able to discriminate between fertile and subfertile individuals.Alternatively,while the percentage of progressively motile sperm showed a significant predictive value,with an AUC of 0.73(P=0.05),CMA3/Yo-Pro-1 staining even depicted superior results for the prediction of in vivo fertility in Bulls.Specifically,the percentage of viable sperm with poor chromatin condensation showed better accuracy and precision to predict in vivo fertility,wi

关键词： Bull Chromatin Chromomycin A3 Condensation Fertility Flow cytometry Sperm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of enzymatic and non-enzymatic antioxidants in diluents on cryopreserved Bull epididymal sperm

引用

Asian pacific Journal of Reproduction 2022年第1期11卷 44-50页

作者： Hasan Sakhdary Abbas Farshad Jalal Rostamzadeh Fateme Bahri Binabaj Keyvan Sobhani Laboratory of Reproduction Biology Department of Animal SciencesFaculty of AgricultureUniversity of KurdistanSanandajKurdistanIran Department of Animal Sciences Faculty of AgricultureGonbad Kavous UniversityGonbad KavousGorganIran

Objective:To evaluate the supplementation effects of vitamin E,vitamin C,superoxide dismutase(SOD),and catalase to diluents on Bull cryopreserved epididymal sperm.Methods:Sperm were retrieved from 20 Bull testes and were then supplemented with 0.1 mM vitamin E,5.0 mM vitamin C,100.0 IU/mL SOD,and 100.0μg/mL catalase alone,or in a combination.The control treatment contained no addition.After supplementation,samples were frozen and stored in liquid nitrogen.The sperm parameters including motility,progressive motility,viability,acrosome integrity,plasma membrane integrity,kinematics and DNA damage were evaluated following the thawing process.Results:Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment(PBull epididymal sperm and could be used for cryopreservation.

关键词： Antioxidant Cryopreservation Spermatological characteristics DNA damage Bull Vitamin E Vitamin C Superoxide dismutase Catalase Oxidative stress

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Testicular vascularization at two locations in relation to hormonal levels,and pixel echotexture in Bulls at different ages

引用

Asian pacific Journal of Reproduction 2022年第4期11卷 193-200页

作者： Elshymaa A.Abdelnaby Ibrahim A.Emam Department of Theriogenology Faculty of Veterinary MedicineCairo UniversityEgypt Department of Surgery and RadiologyFaculty of Veterinary MedicineCairo UniversityGizaEgypt

Objective:To assess the hemodynamic changes that occurred in the main testicular artery at two different locations such as supra and marginal ones in Bulls at three different ages.Methods:Eighteen Baladi Bulls were divided according to their age into three different age groups:1 year[n=6;groupⅠwith bodyweight(380±10)kg],3 years[n=6;groupⅡwith bodyweight(570±10)kg],and 6 years old[n=6;groupⅢwith bodyweight(650±10)kg]Baladi Bulls.Circumference of the scrotum,thickness of the mediastinum,testicular dimensions,vascularity,and hormonal levels were measured.Testicular hemodynamics were assessed by Doppler ultrasound scanning.Results:Testicular width,length,and volume were significantly different among the three age groups,with the highest in Bulls of groupⅢ(PBulls in groupⅢhad significantly higher mediastinal line thickness and scrotal circumference than groupⅠ(PBulls in groupⅡshowed a decline in Doppler indices(resistance and pulsatility indexes)at both testicular branches compared to the other two groups(PBull has the lesser Doppler indices with lesser echotexture and greater nitric oxide with estradiol concentrations,which d

关键词： Bull Doppler indices End diastolic velocity Estradiol Testicular blood flow Resistance index Pulsatility index

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and Bull Leydig cells

引用

Asian Journal of Andrology 2005年第4期7卷 411-417,页

作者： Paul F.Moundipa Nathalie Sara E.Beboy Fabien Zelefack Silvère Ngouela Etienne Tsamo Wolf-Bernhard Schill Thomas K.Monsees Departments of Biochemistry Organic Chemistry University of Yaounde IP.O.Box 812 YaoundeCameroon Centre for Dermatology and Andrology Justus Liebig UniversityGaffkystr.1435385 GiessenGermany Centre for Dermatology and Andrology Justus Liebig UniversityGaffkystr.1435385 GiessenGermany Department of AnatomyUniversity of TechnologyFetscherstr.7401307 DresdenGermany

Aim： To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the Bull, and to develop a novel in vitro test system using Leydig cells from Bull testes. Methods： The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the Bull was analyzed using ^125I-radioimmunoassay （^125I-RIA）. Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from Bull testes. Results： Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin （hCG）. Basella alba extracts significantly enhanced testosterone production in Bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations. Conclusion： Leydig cells purified from Bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.

关键词： Bull rats Leydig cells testosterone Basella alba Hibiscus macranthus plant extract

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pulse wave Doppler ultrasound of testicular arteries and their relationship with semen characteristics in healthy Bulls

引用

Journal of Animal Science and Biotechnology 2018年第2期9卷 319-325页

作者： Alessia Gloria Augusto Carluccio Laura Wegher Domenico Robbe Claudio Valorz Alberto Contri Faculty of Veterinary Medicine University of TeramoLocalitàPiano D’Accio64100 TeramoItaly Provincial Breeders Federation of Trento Via delle Bettine 4038121 TrentoItaly

Background: Semen evaluation is used to estimate the testicular function. In Bulls, the spermatozoa present in the ejaculate are the result of a process that begun more than 2 mo earlier, bequeathing a delayed depiction of the actual function of the testis. Since testis vascularization might be critical for the gonad function, selected pulse wave Doppler ultrasound parameters were assessed in this study, for instance the peak systolic velocity, the end diastolic velocity and the resistive index of the testicular artery along the spermatic cord, the marginal portion of the testicular artery and the intratesticular branches of the testicular artery both in healthy adult and young Bulls.Correlations between these parameters and characteristics of semen that was collected numerous times, before and after the Doppler ultrasound examination.Results: The peak systolic velocity and the end diastolic velocity measured in the testicular artery along the spermatic cord(supratesticular artery – SA) were variable among the Bulls and within individual Bulls, likely due to the convoluted course of the vessel. The resistive index was found highly repeatable in the same Bull. A reduction in the resistive index was found between the supratesticular artery and the marginal portion of the testicular artery(P Bulls compared with adults. A significant correlation was found between the resistive index of the marginal portion of the testicular artery and total sperm in the ejaculate(r = 0.516, P < 0.05), the immature sperm(r = 0.462, P < 0.05), the teratoid sperm(r = 0.375, P < 0.05), and the "Dag defect" sperm(r = 0.389, P < 0.05). Similarly, the resistive index of the intratesticular branches of the testicular artery were found correlated with the total sperm number in the ejaculate(r = 0.568, P < 0.05), the immatur

关键词： Bull Pulse wave Doppler Sperm morphology Testis Ultrasound

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computer-assisted sperm morphometry fluorescence-based analysis has potential to determine progeny sex

引用

Asian Journal of Andrology 2016年第6期18卷 858-862页

作者： Pilar Santolaria Alfredo Pauciullo Miguel A Silvestre Sandra Vicente-Fiel Leyre Villanova Alain Pinton Juan Viruel Ester Sales Jesus L Yaniz TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte SIN 22071 Huesca Spain Department of Agriculture Forest and Food Sciences University of Torino Largo Paolo Braccini 2 10095 Grugliasco TO Italy Department of Functional Biology and Physical Anthropology University of Valencia 469100 Burjassot Valencia Spain UMR 1388 INRA-ENVT INPT Animal Cytogenetis GenPhySE National veterinary school of Toulouse 23 Chemin des Capelles 31076 Toulouse Cedex 3 France Mediterranean Institute of marine and terrestrial Biodiversity and Ecology (IMBE) University of Aix Marseille Chemin de la Batterie des Lions 13007 Marseille France TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Agricultural and Environmental Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte S/N 22071 Huesca Spain

This study was designed to determine the ability of computer-assisted sperm morphometry analysis （CASA-Morph） with fluorescence to discriminate between spermatozoa carrying different sex chromosomes from the nuclear morphometrics generated and different statistical procedures in the bovine species. The study was divided into two experiments. The first was to study the morphometric differences between X- and Y-chromosome-bearing spermatozoa （SX and SY, respectively）. Spermatozoa from eight Bulls were processed to assess simultaneously the sex chromosome by FISH and sperm morphometry by fluorescence-based CASA-Morph. SX cells were larger than SY cells on average （P 〈 0.001） although with important differences between Bulls. A simultaneous evaluation of all the measured features by discriminant analysis revealed that nuclear area and average fluorescence intensity were the variables selected by stepwise discriminant function analysis as the best discriminators between SX and SY. In the second experiment, the sperm nuclear morphometric results from CASA-Morph in nonsexed （mixed SX and SY） and sexed （SX） semen samples from four Bulls were compared. FISH allowed a successful classification of spermatozoa according to their sex chromosome content. X-sexed spermatozoa displayed a larger size and fluorescence intensity than nonsexed spermatozoa （P 〈 0.05）. We conclude that the CASA-Morph fluorescence-based method has the potential to find differences between X- and Y-chromosome-bearing spermatozoa in bovine species although more studies are needed to increase the precision of sex determination by this technique.

关键词： Bull computer-assisted sperm morphometry analysis sexing sperm morphometry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein Bull testis and are correlated with sperm quality

引用

BIOCELL 2021年第1期45卷 79-87页

作者： YONG LIU ZHIHUA JU QIANG JIANG WENHAO LIU CHUNHONG YANG YARAN ZHANG XIUGE WANG YAPING GAO XIAOCHAO WEI YAN SUN JINPENG WANG MINGHAI HOU LING YANG JINMING HUANG Dairy Cattle Research Center Shandong Academy of Agricultural SciencesJinan250100China College of Life Sciences and Food Engineering Hebei University of EngineeringHandan056021China

F-box and WD-40 domain protein 11(FBXW11)is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of spermatogenesis.In our previous research,the mRNA expression of FBXW11 in Bull sperm with high motility is significantly higher than that with low motility.In the present study,the protein expression levels of FBXW11 in Bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance groups.The immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous tubules.Three splice variants of the FBXW11 gene,namely,FBXW11-tv1,FBXW11-tv2,and FBXW11-tv3,were identified in testicular tissues.The splicing patterns of the three variants are exon skipping.The transcript FBXW11-tv2 expressions were the highest in each sample.The low-performance groups displayed higher FBXW11-tv1 and FBXW11-tv2 transcript expressions than the normal performance groups.Two CpG islands were located within the 5’UTR and exon 1-2 region of the FBXW11 gene.Bisulfite sequencing PCR results demonstrated that the methylation levels of 11 methylation sites in the CpG island 2 from−99 to−43 in the normal performance groups were significantly lower than those in the low-performance groups.Pearson correlation analysis suggested that the CpG island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression level.Our data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and were correlated with sperm quality traits during spermatogenesis in Holsteins.

关键词： FBXW11 Alternative splicing DNA methylation Bull Sperm quality traits

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Polymyxin B effects on motility parameters of cryopreserved Bull semen

引用

Asian pacific Journal of Reproduction 2017年第1期6卷 38-45页

作者： Mojtaba Rashedi Mohammad Hashem Fazeli Hamid Gholami Mohammad Bahreini Young Researchers and Elites Club Faculty of Veterinary MedicineIslamic Azad UniversityShahrekord BranchShahrekordIran Department of Clinical Science Faculty of Veterinary ScienceIslamic Azad UniversityShahrekord BranchShahrekordIran Jahed Animal Institutions Company KarajAlborz provinceIran Embryology laboratory Interbreeding CenterKarajAlborz provinceIran

Objective:To evaluate the effect of adding different values of polymyxin B (PMB) to Bull semen on various motility parameters of post-thawed semen such as total motility, progressive motility and velocity parameters using kinetic parameters of sperm by Computer Assisted Sperm Analysis.Methods: Gram negative bacteria release lipopolysaccharide, which induces the apoptotic pathway. Antibiotics are added to semen in order to prevent bacterial contaminations in bovine semen. These antibiotics kill the bacteria especially gram negative bacteria. Therefore, their endotoxins are released during bacteriolysis and bind to the head region and midpiece of sperm. PMB is a bactericidal antibiotic against multidrug resistant gram-negative bacteria and is able to neutralize the toxic effects of the released endotoxin. This study was performed on 3-year old Taleshi Bulls. Results:The results showed both positive and negative significant effects of PMB on semen quality. Total motility and progressive motility were significantly increased (PBull semen is advised, but before using it, the rate of endotoxins must be measured.Conclusions: We advise using PMB after measuring endotoxin concentration;In vitro,in vivoand in field fertilization, adding other sperm evaluation factors such as acrosomal integrity, DNA integrity, mitochondrial function to PMB treated semen.

关键词： Taleshi Bull semen Polymyxin B Computer Assist Semen Analyzer Motility parameters

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Factors affectingin-vivo fertility of crossbred Egyptian - Italian buffalo semen

引用

Asian pacific Journal of Reproduction 2017年第6期6卷 264-267页

作者： GA El-Sisy KGhM Mahmoud AAE El-sokary MF Nawito YF Ahmed Department of Animal Reproduction and A.I. National Research CenterDokkiGizaEgypt General Organization for Veterinary Services DokkiEgypt

Objective:To assess the effect of region, season and year of insemination onin-vivo fertility of Italian-Egyptian crossbred buffalo semen.Methods: A total number of 4799 female buffaloes were inseminated by frozen semen with at least 50% post-thaw motility of Egyptian-Italian crossbred Bulls in three localities in Delta, lower Egypt (El-Behira, El-Sharkia and Damietta) during the period of 2013, 2014 and 2015. The pregnancy rate after two months was evaluated during the four seasons.Results:The rate of pregnancy was significantly (P<0.0001) differ among the three localities. The effect of year of insemination on pregnancy rate was significantly higher during 2014 and 2015 than 2013 in El-Sharkia and El-Behira. But in Damietta, the rate of pregnancy was significantly higher in 2014 than 2013 and 2015. There were no significant differences among seasons in El-Behira and Damietta governorates but there was significant (P<0.05) differences in pregnancy rate in El-Sharkia. It was higher in summer, spring and autumn than in winter.Conclusions:Localities, year of insemination and season of the year have effects on fertility of crossbred Egyptian-Italian buffalo semen.

关键词： Artificial insemination Pregnancy rate Egyptian-Italian crossbred Buffalo Bull

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Association of Osteopontin Gene Promoter Single Nucleotide Polymorphisms with Bull Semen Quality

引用

Advances in Reproductive Sciences 2016年第1期4卷 1-7页

作者： Rick W. Rorie Chance L. Williams Toby D. Lester Department of Animal Science University of Arkansas Fayetteville AR USA

Osteopontin (OPN) is a protein found at higher concentrations in the seminal plasma of Bulls with above average fertility. Polymorphisms have been reported within the OPN gene promoter that can affect production of this protein and thus, affect fertility. Therefore, Angus (n = 5) and Angus x Gelbvieh (Balancer, n = 14) and Angus x Brahman (n = 15) Bulls were evaluated for presence of single nucleotide polymorphisms (SNP) in the Bos taurus OPN gene (GenBank: AY878328.1) promoter region, and their possible effects on Bull semen quality as evaluated by computer-assisted semen analysis (CASA). Semen was collected by electroejaculation 6 to 9 times from each Bull, and each semen collection was evaluated by CASA for motile, progressive and rapid sperm within 5 mins of ejaculation. The Bulls were genotyped for reported single nucleotide polymorphisms (SNP) in the promoter region of the OPN gene through amplification of two 700 base pair (bp) DNA fragments and sequencing of the resulting PCR products. Seven SNP sites were identified, at bp 3379, 3490, 3492, 5075, 5205, 5209, and 5263 of the OPN gene. The SNP identified at bp 5205, 5209 and 5263 had not been previously reported. Individual SNP sites were evaluated as the main effect on CASA sperm motility variables in a SAS MIXED model for repeated measures. A thymine to guanine substitution at bp 3379 was associated with increased (P ≤ 0.02) percentage of motile, progressive and rapid sperm in Angus x Brahman Bulls, and tended (P ≤ 0.10) to increase the same sperm motility parameters in Angus, and Angus x Gelbvieh Bulls. The percentages of motile, progressive and rapid sperm were similar (P ≥ 0.05) among genotypes for the other 6 SNP identified. These results suggest that identification and genotyping of polymorphisms within the promoter region of the bovine OPN gene may be useful for selecting Bulls with improved sperm motility parameters.

关键词： Osteopontin Polymorphism Sperm Motility Bull

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：