DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein bull testis and are correlated with sperm quality
作者机构：Dairy Cattle Research CenterShandong Academy of Agricultural SciencesJinan250100China College of Life Sciences and Food EngineeringHebei University of EngineeringHandan056021China
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2021年第45卷第1期
页 面：79-87页
核心收录：
学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by the Major Project of National Transgene in China(2018ZX08007001-002) the National Natural Science Foundation of China(31671286,31672397,31771374) Shandong Provincial Natural Science Foundation for Distinguished Young Scholars of China(JQ201709) the Program of National Cow Industrial Technology System of China(CARS-37) the Shandong Provincial Key Research and Development Program of China(2017GNC10120)
主 题：FBXW11 Alternative splicing DNA methylation Bull Sperm quality traits
摘 要：F-box and WD-40 domain protein 11(FBXW11)is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of *** our previous research,the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low *** the present study,the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance *** immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous *** splice variants of the FBXW11 gene,namely,FBXW11-tv1,FBXW11-tv2,and FBXW11-tv3,were identified in testicular *** splicing patterns of the three variants are exon *** transcript FBXW11-tv2 expressions were the highest in each *** low-performance groups displayed higher FBXW11-tv1 and FBXW11-tv2 transcript expressions than the normal performance *** CpG islands were located within the 5’UTR and exon 1-2 region of the FBXW11 *** sequencing PCR results demonstrated that the methylation levels of 11 methylation sites in the CpG island 2 from−99 to−43 in the normal performance groups were significantly lower than those in the low-performance *** correlation analysis suggested that the CpG island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression *** data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and were correlated with sperm quality traits during spermatogenesis in Holsteins.
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