Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Leydig cells

作     者：Paul F.Moundipa Nathalie Sara E.Beboy Fabien Zelefack Silvère Ngouela Etienne Tsamo Wolf-Bernhard Schill Thomas K.Monsees 

作者机构：Departments of Biochemistry Organic ChemistryUniversity of Yaounde IP.O.Box 812 YaoundeCameroon Centre for Dermatology and AndrologyJustus Liebig UniversityGaffkystr.1435385 GiessenGermany Centre for Dermatology and AndrologyJustus Liebig UniversityGaffkystr.1435385 GiessenGermany Department of AnatomyUniversity of TechnologyFetscherstr.7401307 DresdenGermany 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2005年第7卷第4期

页      面：411-417,页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：grants from the International Foundation for Science(PFM, F/2286-2) the DFG (TKM, 445 KAM-18/2/03). We are grateful to Mr Tchouanguep Cé1estin for providing the plant material 

主　　题：bull rats Leydig cells testosterone Basella alba Hibiscus macranthus plant extract 

摘      要：Aim： To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes. Methods： The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using ^125I-radioimmunoassay （^125I-RIA）. Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes. Results： Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin （hCG）. Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations. Conclusion： Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.