Effects of enzymatic and non-enzymatic antioxidants in diluents on cryopreserved bull epididymal sperm

作     者：Hasan Sakhdary Abbas Farshad Jalal Rostamzadeh Fateme Bahri Binabaj Keyvan Sobhani Hasan Sakhdary;Abbas Farshad;Jalal Rostamzadeh;Fateme Bahri Binabaj;Keyvan Sobhani

作者机构：Laboratory of Reproduction BiologyDepartment of Animal SciencesFaculty of AgricultureUniversity of KurdistanSanandajKurdistanIran Department of Animal SciencesFaculty of AgricultureGonbad Kavous UniversityGonbad KavousGorganIran 

出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)

年 卷 期：2022年第11卷第1期

页      面：44-50页

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：Antioxidant Cryopreservation Spermatological characteristics DNA damage Bull Vitamin E Vitamin C Superoxide dismutase Catalase Oxidative stress 

摘      要：Objective:To evaluate the supplementation effects of vitamin E,vitamin C,superoxide dismutase(SOD),and catalase to diluents on bull cryopreserved epididymal ***:Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E,5.0 mM vitamin C,100.0 IU/mL SOD,and 100.0μg/mL catalase alone,or in a *** control treatment contained no *** supplementation,samples were frozen and stored in liquid *** sperm parameters including motility,progressive motility,viability,acrosome integrity,plasma membrane integrity,kinematics and DNA damage were evaluated following the thawing ***:Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment(P0.05).While compared to the control treatment,vitamin C had no improvement effect on sperm characteristics except for membrane *** of vitamin E+vitamin C had a significant improvement in total motility,progressive motility,viability,membrane and acrosome integrity compared to the control and other treatments(P0.05).Compared to the control treatment,addition of SOD or catalase alone significantly improved the percentages of total motility,progressive motility,viability,membrane and acrosome integrity(P0.05).Furthermore,SOD+catalase significantly increased total motility,progressive motility,viability,acrosome and membrane integrity characteristics compared to the catalase treatment(P0.05).Vitamin E alone,vitamin E+vitamin C,and SOD in diluents decreased DNA damages and thereby improved the rate of intact sperm ***:Addition of 100.0 IU/mL SOD alone and 0.1 mM vitamin E+5.0 mM vitamin C,and also 5.0 mM vitamin C+100μg/mL catalase in a combination improves the quality of cryopreserved bull epididymal sperm and could be used for cryopreservation.