Sperm chromatin condensation as an in vivo fertility biomarker in bulls:a flow cytometry approach

作     者：Marc Llavanera Jordi Ribas-Maynou Ariadna Delgado-Bermúdez Sandra Recuero Rodrigo Muiño Carlos OHidalgo Carolina Tamargo Sergi Bonet Yentel Mateo-Otero Marc Yeste Marc Llavanera;Jordi Ribas-Maynou;Ariadna Delgado-Bermúdez;Sra Recuero;Rodrigo Mui?o;Carlos O.Hidalgo;Carolina Tamargo;Sergi Bonet;Yentel Mateo-Otero;Marc Yeste

作者机构：Biotechnology of Animal and Human Reproduction(TechnoSperm)Faculty of SciencesUniversity of GironaC/Maria Aurèlia Campany69ES-17003 GironaSpain Department of BiologyUnit of Cell BiologyFaculty of SciencesUniversity of GironaES-17003 GironaSpain Department of Animal PathologyFaculty of Veterinary MedicineUniversity of Santiago de CompostelaES-15705 LugoSpain Department of Animal Selection and ReproductionThe Regional Agri-Food Research and Development Service of Asturias(SERIDA)E-33394 GijónSpain 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2022年第13卷第2期

页      面：418-429页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：the support from the Ministry of Science Innovation and Universities,Spain (AGL2017–88329-R and FPU18/00666) Regional Government of Catalonia,Spain (2017-SGR-1229) University of Girona (Postdoc-Ud G2020) 

主　　题：Bull Chromatin Chromomycin A3 Condensation Fertility Flow cytometry Sperm 

摘      要：Background:Genetic selection in cattle has been directed to increase milk ***,coupled to the fact that the vast majority of bovine artificial inseminations(AI)are performed using cryopreserved sperm,have led to a reduction of fertility rates over the ***,seeking sensitive and specific sperm biomarkers able to predict fertility rates is of vital importance to improve cattle reproductive *** humans,sperm chromatin condensation evaluated through chromomycin A3(CMA3)has recently been purported to be a powerful biomarker for sperm functional status and male *** objectives of the present study were:a)to set up a flow cytometry method for simultaneously evaluating chromatin condensation and sperm viability,and b)to test whether this parameter could be used as a predictor of in vivo fertility in *** study included pools of three independent cryopreserved ejaculates per bull from 25 Holstein *** outcomes of each sire were determined by non-return rates,which were used to classify bulls into two groups(highly fertile and subfertile).Results:Chromatin condensation status of bovine sperm was evaluated through the combination of CMA3 and Yo-Pro-1 staining and flow *** quality parameters(morphology,viability,total and progressive motility)were also *** correlation coefficients and ROC curves were calculated to assess their capacity to predict in vivo *** morphology,viability and total motility presented an area under the ROC curve(AUC)of 0.54,0.64 and 0.68,respectively(P0.05),and thus were not able to discriminate between fertile and subfertile ***,while the percentage of progressively motile sperm showed a significant predictive value,with an AUC of 0.73(P=0.05),CMA3/Yo-Pro-1 staining even depicted superior results for the prediction of in vivo fertility in ***,the percentage of viable sperm with poor chromatin condensation showed better accura