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Effect of Mushroom Extract Hericium erinaceus on Spatial Memory and Morphology of Neurons in the CA1 and CA3 Regions of the Hippocampus in ovariectomized rats

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Open Journal of Veterinary Medicine 2024年第7期14卷 163-179页

作者： Lucia Garcia-Delgado Mariana Monserrath Verdía-Venegas Jacinto Bañuelos-Pineda Esther Albarrán-Rodríguez Guillermo Nolasco-Rodriguez José Ricardo Cuellar-Pérez Sergio Fausto-Guerra Manuel Rosales-Cortés Master in Behavioral Sciences Institute of Neurosciences University of Guadalajara Zapopan Mexico Department of Veterinary Medicine Division of Veterinary Sciences CUCBA University of Guadalajara Zapopan Mexico Botany and Zoology Laboratory CUCBA University of Guadalajara Zapopan Mexico

Various studies indicate that low levels of estradiol negatively impact cognitive abilities. Extracts from the fungus Hericium erinaceus (HE) contain bioactive components that promote the proper functioning of the nervous system and potential effects on protection against neurodegenerative diseases, including dementia and motor dysfunctions. The objective was to evaluate the effects of the administration of the HE mushroom extract on visuospatial memory and morphology of neurons in the CA1 and CA3 regions of the hippocampus in ovariectomized rats. 40 young Wistar rats weighing 90 ± 10 g BW were used, which were distributed into four groups of 10 animals;Control Group, non-ovariectomized and untreated rats;Group E2, ovariectomized rats treated with estradiol (2 μg/kg/body weight);Group HE, ovariectomized rats treated with the extract of the fungus Hericium erinaceus (0.5 mg/kg body weight) and Group Ovx/ST, ovariectomized rats, without treatment. The animals were tested in the Barnes and Open Field maze, then they were sacrificed, and their brains were obtained to perform a histological analysis of neuronal morphology in the CA1 and CA3 areas of the hippocampus. The most outstanding results showed that the Ovx/ST group recorded the longest time to arrive at the escape box and stay in the Barnes maze. A correlation was observed between neuronal damage and function;in the groups that did not present satisfactory performance in the maze tests, morphological alterations were identified such as the presence of some neuronal somata with degeneration characteristics such as pyknosis, nuclear basophilia and shrinkage of the cells. Its soma, as well as a decrease in the nuclear area of CA1 and CA3 neurons. It is concluded that the fungus Hericium erinaceus exerted a neuroprotective effect on the neuronal bodies of the hippocampus, associated with better performance in the visuospatial recognition memory test.

关键词： ovariectomized rats Ovarian Hypofunction Estradiol Hericium erinaceus Barnes Circular Labyrinth Open Field Labyrinth

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The Effect of Estrogen on the Restoration of Bone Mass and Bone Quality in ovariectomized rats

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2000年第4期20卷 283-286页

作者：陈璐璐曾天舒夏文芳黎惠青周愍 Depatment of Endocrinology Xiehe Hospital Tongji Medical University Wuhan

To evaluate the effect of estrogen on its ability to restore the bone mass and bone quality in ovariectomized rats by examining the changes of bone morphology and histomorphometry, 3- month-old rats were divided randomly into 4 groups: normal control, ovariectomized (OVX), sham- operated (Sham-O) and OVX plus estrogen (OVX+E2). Treatment initiated from the day 8 weeks after operation and continued for 12 weeks. Bone morphology and histomorphometry were examined afterwards. Results showed that comparing to control group, the trabecular bone in OVX appeared thinner and reduced in the amount. The connectivity between trabecula was decreased and the struc- ture disordered. The free-end of trabecula was increased. The cavity of bone marrow enlarged. After treatment with estrogen, above changes improved remarkably by different degree, although did not reach the normal face. The bone histomorphometry results damonstrated that estrogen treatment in- creased bone mass and the amount of trabecula by 129% and 132% respectively (Povariectomized rats with estrogen can not only increase bone mass, also improve the bone structure and enhance the property of bone mechanics.

关键词： estrogen ovariectomized rats morphology histomorphometry osteoporosis

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Activities of the Deriviatives of Chitin on the Osbeoblast Proliferation and the Effect on Bone Strength in ovariectomized rats

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Wuhan University Journal of Natural Sciences 2006年第3期11卷 715-719页

作者： WEI Changzheng LIU Wanshun HAN Baoqin DUAN Yixiang College of Marine Life Sciences Ocean University of China Qingdao 266003 Shandong China Orthopaedics of Qingdao Municipal Hospilal Qingdao 266003 Shandong China

This study intends to examine the effects of different concentrations of four kinds of degradations of chitin： glucosamine （GLC）, N-acetyl-D-glucosamine （NAG）, chitooligosaccharide（COS）, CM-chitooligosaccharide （CM-COS）—on the proliferation of MC3T3-E1 cell line cultured in vitro. Results suggest that all of the glucoses mentioned above promoted the proliferation of osteoblast, and various concentrations have different effects： the proliferation was remarkable when the concentration of GLC, NAG, COS, CM-COS was 100 , 100, 500, 500 μg/mL ,respectively. Furthermore we choose the glucosamine as the material and study the effect on the bone strength in ovariectomized rats. The results showed that the middle does of glucosamine can significantly increase the hone strength of femur in ovariectomized rats.

关键词： glucosamine chitin ovariectomized rats bone strength osteoporosis osteohlast proliferation

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The effect of Wujiadan Recipe on serum sex hormone and expression of Kiss-1, GPR54 and GnRH in arcuate nucleus of hypothalamus in ovariectomized rats

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Journal of Hainan Medical University 2020年第18期26卷 31-34页

作者： Ming-Yu Zhao Wen-Ying Niu Bo Lv Zuo-Mei Wang Xin-Hui Yao Hong-Bin Xiao Heilongjiang University of Traditional Chinese Medicine Harbin 150040Heilongjiang Heilongjiang Academy of Chinese Medical Sciences Harbin 150036Heilongjiang

Objective:To explore the effect of wujiadan Recipe on the expression of FSH,E2 in serum and KiSS-1,GPR54,GnRH mRNA in hypothalamic arcuate nucleus tissue homogenate of ovariectomized rats,and to further explore the mechanism of wujiadan Recipe on menopausal ***:Sixty-four adult female rats were randomly divided into three groups:blank group,model group,Chinese medicine control group,western medicine control group,and wujiadan high,medium and low dose *** for the blank group,all the other groups were *** rats in the normal group and the model group were given the same dose of saline once a *** rat in the blank group died during the *** 4 weeks of continuous administration,blood was collected from abdominal aorta and brain stereotaxic localization was performed to preserve the arcuate nucleus of *** levels of FSH and E2 in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of Kiss-1,GPR54 and GnRH mRNA in arcuate nucleus of hypothalamus were detected by ***:*** with the blank group,FSH level in the model group was significantly higher(P<0.01),and E2 level was significantly lower(P<0.01).Compared with the model group,the level of serum E2 in the western medicine control group increased significantly(*** serum FSH content in each group decreased,especially in the high dose group and the western medicine control group(P<0.01),and in the Chinese medicine control group,the middle dose group and the low dose group(P<0.05).Compared with the blank group,the expression of KiSS-1 mRNA,GPR54 mRNA and GnRH mRNA in the model group increased significantly(P<0.01);compared with the model group,the expression of KiSS-1 mRNA and GPR54 mRNA in the high dose group,middle dose group and Western medicine control group of wujiadan prescription decreased significantly(P<0.05).Compared with the model group,the expression of GnRH mRNA in the high dose group and the western medi

关键词： Wujiadan recipe Menopausal syndrome ovariectomized rats Sex hormone Kiss-1 GPR54 GnRH

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Effects of treadmill training and detraining on lumbar vertebra bone of ovariectomized rats

Effects of treadmill training and detraining on lumbar verte...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：卜淑敏韩天雨杨楠首都体育学院运动科学与健康学院

Introduction:This study aims to determine whether the prevention effects of treadmill training on bone loss induced by estrogen deficiency can be preserved after ***:Sixty female Sprague-Dawley rats were randomly divided into three groups:sham-operation group（Sham）,ovariectomized group（OVX）,ovariectomized and exercise group（EX）.After regular training,the rats in each group were randomly divided into two subgroups namely:Sham-16 and Sham-32,OVX-16 and OVX-32 as well as EX and *** the end of training or detraining,the lumbar vertebrate BMD was detected by DEXA and the bone strength was determined by compressive *** morphological changes of the lumbar vertebrate were observed by HE staining and the protein expression of PPARγand osterix were detected by Western ***:The number of bone marrow adipocytes and PPARγexpression in lumbar vertebrate of OVX-16 group or OVX-32 group were significantly higher than that of in Sham-16 group or Sham-32 group ***,the lumbar vertebrate BMD and strength were significantly *** number of bone marrow adipocytes and PPARγexpression in lumbar vertebrate of EX group were significantly lower than that of in OVX-16 group while the lumbar vertebrae BMD and bone strength were significantly *** all the changes gained by training were reversed by ***:These results suggested that the benefit effects gained by treadmill training in lumbar vertebrae of OVX rats were not preserved after detraining.

关键词： Training and detraining Bone mass Bone biomechanics Bone marrow adipocytes ovariectomized rats

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Effect of APP 17-mer Peptide on Hippocampal Neurodegeneration in ovariectomized rats

Effect of APP 17-mer Peptide on Hippocampal Neurodegeneratio...

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第十五届国际药理学大会

作者： Meng Yan Wang Rong Ji Zhi Juan Sheng Shu Li Neuro - Biochemistry Laboratory Beijing Xuan - Wu Hospital Capital University of Medical Sciences

＜正＞ The objective of this study was to investigate whether hippocampal neurodegenerationexisted in experimental ovariectomized （OVX） rats, and to study the effect of amyloid precursor protein 17 - mer peptide（ APP 17 - mer peptide） on them. The results showed that learning and memory function of OVX rats was damaged, expression of NGF decreased, expression of estrogen receptor - alpha （ER - alpha） increased and mitochondrial swelling occurred in hippocampal neurons. Above changes could be ameliorated by APP17 - mer peptide. though the blood estrogen level showed no change. These results indicated that APP 17 - mer peptide could ameliorate the neurodegeneration due to estrogen deficiency but the mechanism was not through regulation of estrogen level. Our findings suggest that by activating common intracellular signaling pathways and initiating "cross talk" with neurotrophins. APP 17 - mer peptide improves neurodegeneration caused by estrogen deficiency. However, more work will be required to explain the neuroprotection rendered by APP 17 - mer peptide in our model.

关键词： ovariectomized rats neurodegeneration. APP 17 - mer peptide

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Effects of Different Intervention Methods on the Protein Expression of ER and ERRα in the Uterus of ovariectomized Osteoporosis rats

Effects of Different Intervention Methods on the Protein Exp...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：韩天雨王慧彬卜淑敏首都体育学院细胞分子生物实验室

Objective The aim of this study was to compare the effects of different intervention methods on the protein expression of estrogen receptor（ERα,ERβ）and estrogen receptor related receptorα（ERRα）in the uterus of ovariectomized osteoporosis *** Eighty healthy female SD rats were randomly divided into sham-operation（Sham）and ovariectomized（OVX）groups by body *** ten weeks of the operation,the OVX groups were randomly divided into the following six groups by body weight:OVX,17β-estradiol（E2）,Genisteine（G）,treadmill exercise（TE）,Lithium chloride（Li CL）,and Whole-body vertical vibration（WBVV）.Then the rats began to be treated with different intervention methods for *** 36-48 hours of the finally intervention,the blood was took from the abdominal aorta and the protein was extracted from *** the protein expression of ERα,ERβand ERRαwas detected by western *** The uterus weight index and serum E2 were significantly decreased after OVX,but were significantly increased after treatment with *** significant differences were seen after treatment with the other four *** revealed by the western blot results,the protein expression of ERαin the OVX groups were significantly higher than that of in the Sham group while the ERRαexpression was significantly *** protein expression of ERαin E2,TE,and WBVV groups were significantly lower than that of in OVX group while no difference was seen between the G group and OVX *** protein expression of ERRαin E2 and G groups were significantly higher than that of in OVX group while the protein expression of ERRαin TE and WBVV groups were significantly *** significant difference was seen between the Licl group and OVX *** were no significant differences in the protein expression of ERβamong the all *** Apart from G treatment,the other four interventions had inhibitive effects on the protein expression of ERαin uterus of OVX osteoporosis *** TE a

关键词： ovariectomized rats Estrogen receptor Whole-body vertical vibration Genistein Treadmill exercise

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Beneficial effects of melatonin in experimental models of Alzheimer disease

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Acta Pharmacologica Sinica 2006年第2期27卷 129-139页

作者： Yong CHENG~2 Zheng FENG~3 Qing-zhu ZHANG~4 Jun-tian ZHANG~(2.5) ~2Department of Pharmacology,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China:~3Department of Anesthesiology,University of California,San Diego,and Veterans Affairs Medical Center,3350 La Jolla Village Drive,San Diego,CA 92161,USA:~4Department of Pharmacology,College of Pharmacy,Shandong University,Jinan 250012,China InstituteofMateriaMedica ChineseAcademyofMedicalSciencesandPUMCBeijing100050China DepartmentofAnesthesiology UniversityofCaliforniaSanDiegoandVeteransAffairsMedicalCenterUSA CollegeofPharmacy ShandongUniversity

Alzheimer’s disease(AD),a progressive degenerative disorder,is characterizedby the presence of amyloid deposits,neurofibrillary tangles and neuron *** evidence indicates that antioxidants could be useful either for the pre-vention or treatment of *** has been shown that melatonin is a potent antioxi-dant and free radical ***,melatonin stimulates severalantioxidative enzymes and improves mitochondrial energy *** find-ings led us to study amyloid precursor protein transgenic mice,ovariectomizedrats,and pheochromocytoma and astroglioma cell lines,to observe whether mela-tonin had any effect on Alzheimer’s symptoms or pathological *** foundthat melatonin had many beneficial effects in experimental models of AD,includ-ing improvement of cognitive function,anti-oxidative injury,anti-apoptosis,inhi-bition of β-amyloid(Aβ)deposition and Aβ fiber *** groups haveshown that melatonin has an inhibitory effect on tau protein *** actions may potentially slow down or stop the progression of dementia.

关键词： melatonin Alzheimer disease beta-amyloid protein calcium overload APP transgenic mice ovariectomized rats tau protein hyperphosphorylation

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Effects of long-term estrogen replacement therapy on beta-amyloid precursor protein and mRNA expression in ovariectomized rat hippocampus

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Neural Regeneration Research 2009年第1期4卷 48-52页

作者： Bo Jiang Eryuan Liao Liming Tan Ruchun Dai Zhijie Xiao Department of Neurology Xiangya Second Hospital of Central South University Changsha 410011 Hunan Province China Institute of Endocrine and Metabofic Disease Xiangya Second Hospital of Central South University Changsha 410011 Hunan Province China

BACKGROUND： In vitro cultures of neural stem cells have shown that estrogen can regulate beta-amyloid precursor protein （β-APP） metabolism and reduce amyloid-beta production. OBJECTIVE： To investigate the effects of long-term oral administration of compound nylestriol or low-dose 17beta-estradiol on β-APP and mRNA expression in the hippocampus of ovariectomized （OVX） rats. DESIGN, TIME AND SETTING： This randomized and controlled experiment was performed at the Animal Laboratory and Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University between April 2003 and May 2004. MATERIALS： According to body mass, 50 six-month-old female Sprague-Dawley rats were randomly divided into five groups （n = 10 per group）： normal control, sham operation, OVX model, 17beta-estradiol （Sigma, USA）, and compound nylestriol tablet （Laboratory of Endocrine and Metabolic Disease, Xiangya Second Hospital of Central South University） groups. METHODS： rats in OVX plus 17beta-estradiol and OVX plus compound nylestriol tablet groups underwent ovariectomy. On the second day after surgery, rats were intragastrically given 17beta-estradiol （100 μg/kg）, once per day or compound nylestriol tablet （0.5 mg/kg） and levonorgestrel （0.15 mg/kg） every 2 days. MAIN OUTCOME MEASURES： β-APP expression in the hippocampus of OVX rats was determined using immunohistochemistry （SABC method） and β-APP mRNA expression was analyzed by in situ hybridization. The results were quantitatively analyzed using cell counting and average optical density. RESULTS： The number and optical density of β-APP-positive neurons in every subregion of the hippocampus of OVX rats was dramatically increased compared with normal and sham operation groups following 35 weeks of administration （P 〈 0.05）. Levels of β-APP were decreased following oral administration of compound nylestriol or 17beta-estradiol. In situ hybridization showed that long-term estrogen deficiency and oral administra

关键词： ovariectomized rats compound nylestriol tablet 17beta-estradiol cerebral hippocampus beta-amyloid precursor protein

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Effect of Oviductus Ranae and Oviductus Ranae Eggs on Bone Metabolism and Osteoporosis

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Chinese Journal of Integrative Medicine 2013年第7期19卷 532-538页

作者：王丹辉吴巍田建明王照辉王丹彤项颗朱国英韩铁 Traditional Chinese Medicine Academy of Science of Jilin Province Beijing Tongrentang Biological Product Development Co. Ltd. Institute of Radiation Medicine Fudan University Institute of Military Veterinary Academy of Military Medical Sciences

To evaluate the roles or effects of oviductus ranae （OR） or oviductus ranae eggs （ORE） in preventing and treating postmenopausal osteoporosis. Methods： In vivo experiment： Sixty female adult Wistar rats were randomly divided into 5 groups of 12. To provide an osteoporosis model 4 groups of rats were ovariectomized （OVX）, with the 5th being sham operated. Medication commenced 7 days after the operation and lasted continuously for 12 weeks. Sham operated and OVX groups were given equivalent volumes of 5% Tween-80. The other three groups intragastrically received conjugated estrogens （CE）, OR or ORE of the corresponding doses. At the 12th week, serum estrogen, bone gla protein （BGP）, serum calcium, phosphorus, and alkaline phosphatase （ALP） were assayed; bone mineral densities （BMD） were measured and bone scanning was conducted; uteri were weighed, and weight, volume and length of the femoral bones were determined; and cortical thickness of femoral heads and area of bone trabecula were measured by image analyzer. In vitro experiment： Eighty 10-month old SD rats, with equal numbers of mates and females, were randomly divided into 8 groups. Osteoblasts were isolated from neonatal rat calvariae, and the cells were exposed to various concentrations of serum from OR and ORE groups to study the impact of these sera on osteoblastic proliferation, ALP activity and mineralization. Osteoclastic numbers were determined using tartrate resistant acid phosphatase （TRAP）. Results： In vivo experiment： The body weight of the four OVX groups increased significantly （P〈0.01）. Uterine weight of the CE group was the highest （P〈0.01）; Compared with the model group, estrogen level, BMD, bone scanning/bone imaging index weight of the femoral bones, cortical thickness of femoral heads in the OR and ORE groups increased significantly （P〈0.05, P〈0.01）; femoral volume in the ORE group increased significantly （P〈0.05）; and the content of osteocalcin, phosphorus, and ALP in serum decreased signific

关键词： oviductus ranae oviductus ranae eggs ovariectomized rats osteoblasts osteoclasts,osteoporosis

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