The effect of Wujiadan Recipe on serum sex hormone and expression of Kiss-1, GPR54 and GnRH in arcuate nucleus of hypothalamus in ovariectomized rats
作者机构：Heilongjiang University of Traditional Chinese MedicineHarbin 150040Heilongjiang Heilongjiang Academy of Chinese Medical SciencesHarbin 150036Heilongjiang
出 版 物：《Journal of Hainan Medical University》 (海南医学院学报（英文版）)
年 卷 期：2020年第26卷第18期
页 面：31-34页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Scientific research project of Heilongjiang Provincial Health and Health Commission(No.2019-085)
主 题：Wujiadan recipe Menopausal syndrome Ovariectomized rats Sex hormone Kiss-1 GPR54 GnRH
摘 要：Objective:To explore the effect of wujiadan Recipe on the expression of FSH,E2 in serum and KiSS-1,GPR54,GnRH mRNA in hypothalamic arcuate nucleus tissue homogenate of ovariectomized rats,and to further explore the mechanism of wujiadan Recipe on menopausal ***:Sixty-four adult female rats were randomly divided into three groups:blank group,model group,Chinese medicine control group,western medicine control group,and wujiadan high,medium and low dose *** for the blank group,all the other groups were *** rats in the normal group and the model group were given the same dose of saline once a *** rat in the blank group died during the *** 4 weeks of continuous administration,blood was collected from abdominal aorta and brain stereotaxic localization was performed to preserve the arcuate nucleus of *** levels of FSH and E2 in serum were detected by enzyme-linked immunosorbent assay(ELISA),and the expression of Kiss-1,GPR54 and GnRH mRNA in arcuate nucleus of hypothalamus were detected by ***:*** with the blank group,FSH level in the model group was significantly higher(P0.01),and E2 level was significantly lower(P0.01).Compared with the model group,the level of serum E2 in the western medicine control group increased significantly(*** serum FSH content in each group decreased,especially in the high dose group and the western medicine control group(P0.01),and in the Chinese medicine control group,the middle dose group and the low dose group(P0.05).Compared with the blank group,the expression of KiSS-1 mRNA,GPR54 mRNA and GnRH mRNA in the model group increased significantly(P0.01);compared with the model group,the expression of KiSS-1 mRNA and GPR54 mRNA in the high dose group,middle dose group and Western medicine control group of wujiadan prescription decreased significantly(P0.05).Compared with the model group,the expression of GnRH mRNA in the high dose group and the western medi
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