Effects of Different Intervention Methods on the Protein Expression of ER and ERRα in the Uterus of Ovariectomized Osteoporosis Rats
作者单位：首都体育学院细胞分子生物实验室
会议名称：《中国生理学会第24届全国会员代表大会暨生理学学术大会》
会议日期：2014年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
关 键 词：Ovariectomized rats Estrogen receptor Whole-body vertical vibration Genistein Treadmill exercise
摘 要：Objective The aim of this study was to compare the effects of different intervention methods on the protein expression of estrogen receptor（ERα,ERβ）and estrogen receptor related receptorα（ERRα）in the uterus of ovariectomized osteoporosis *** Eighty healthy female SD rats were randomly divided into sham-operation（Sham）and ovariectomized（OVX）groups by body *** ten weeks of the operation,the OVX groups were randomly divided into the following six groups by body weight:OVX,17β-estradiol（E2）,Genisteine（G）,treadmill exercise（TE）,Lithium chloride（Li CL）,and Whole-body vertical vibration（WBVV）.Then the rats began to be treated with different intervention methods for *** 36-48 hours of the finally intervention,the blood was took from the abdominal aorta and the protein was extracted from *** the protein expression of ERα,ERβand ERRαwas detected by western *** The uterus weight index and serum E2 were significantly decreased after OVX,but were significantly increased after treatment with *** significant differences were seen after treatment with the other four *** revealed by the western blot results,the protein expression of ERαin the OVX groups were significantly higher than that of in the Sham group while the ERRαexpression was significantly *** protein expression of ERαin E2,TE,and WBVV groups were significantly lower than that of in OVX group while no difference was seen between the G group and OVX *** protein expression of ERRαin E2 and G groups were significantly higher than that of in OVX group while the protein expression of ERRαin TE and WBVV groups were significantly *** significant difference was seen between the Licl group and OVX *** were no significant differences in the protein expression of ERβamong the all *** Apart from G treatment,the other four interventions had inhibitive effects on the protein expression of ERαin uterus of OVX osteoporosis *** TE a