Effect of Oviductus Ranae and Oviductus Ranae Eggs on Bone Metabolism and Osteoporosis

作     者：王丹辉 吴巍 田建明 王照辉 王丹彤 项颗 朱国英 韩铁 

作者机构：Traditional Chinese Medicine Academy of Science of Jilin Province Beijing Tongrentang Biological Product Development Co.Ltd. Institute of Radiation MedicineFudan University Institute of Military VeterinaryAcademy of Military Medical Sciences 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2013年第19卷第7期

页      面：532-538页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Post-Doctoral Fund of China(No.20090451502) 

主　　题：oviductus ranae oviductus ranae eggs ovariectomized rats osteoblasts osteoclasts,osteoporosis 

摘      要：To evaluate the roles or effects of oviductus ranae （OR） or oviductus ranae eggs （ORE） in preventing and treating postmenopausal osteoporosis. Methods： In vivo experiment： Sixty female adult Wistar rats were randomly divided into 5 groups of 12. To provide an osteoporosis model 4 groups of rats were ovariectomized （OVX）, with the 5th being sham operated. Medication commenced 7 days after the operation and lasted continuously for 12 weeks. Sham operated and OVX groups were given equivalent volumes of 5% Tween-80. The other three groups intragastrically received conjugated estrogens （CE）, OR or ORE of the corresponding doses. At the 12th week, serum estrogen, bone gla protein （BGP）, serum calcium, phosphorus, and alkaline phosphatase （ALP） were assayed; bone mineral densities （BMD） were measured and bone scanning was conducted; uteri were weighed, and weight, volume and length of the femoral bones were determined; and cortical thickness of femoral heads and area of bone trabecula were measured by image analyzer. In vitro experiment： Eighty 10-month old SD rats, with equal numbers of mates and females, were randomly divided into 8 groups. Osteoblasts were isolated from neonatal rat calvariae, and the cells were exposed to various concentrations of serum from OR and ORE groups to study the impact of these sera on osteoblastic proliferation, ALP activity and mineralization. Osteoclastic numbers were determined using tartrate resistant acid phosphatase （TRAP）. Results： In vivo experiment： The body weight of the four OVX groups increased significantly （P〈0.01）. Uterine weight of the CE group was the highest （P〈0.01）; Compared with the model group, estrogen level, BMD, bone scanning/bone imaging index weight of the femoral bones, cortical thickness of femoral heads in the OR and ORE groups increased significantly （P〈0.05, P〈0.01）; femoral volume in the ORE group increased significantly （P〈0.05）; and the content of osteocalcin, phosphorus, and ALP in serum decreased signific