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Nanotechnology and Precision Engineering 2024年第2期7卷 1-10页

作者： Yan Yang Shihong Xu Yu Deng Yaoyao Liu Kui Zhang Shiya Lv Longze Sha Qi Xu Xinxia Cai Jinping Luo State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural *** chip was designed based on the specific structure of neurons and the requirements for detection and ***-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic *** employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated *** hippocampal neurons were cultured on the chip,forming a well-defined neural *** electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode *** study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.

关键词： Microfluidic microelectrode array In vitro Neural chip

microelectrode array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial

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Journal of Materials Science & Technology 2016年第1期32卷 89-96页

作者： Ting Huang ZhonghaiWang Lina Wei Mark Kindy Yufeng Zheng Tingfei Xi Bruce Z. Gao Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China Department of Bioengineering Clemson University Clemson SC29634 USA Departments of Neurosciences and Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston SC29466 USA Ralph H. Johnson VA Medical Center Charleston SC29403 USA Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China Shenzhen Key Laboratory of Human Tissue Regeneration and Repair Shenzhen Institute Peking University Shenzhen 518057 China

Magnesium （Mg）-based biomaterials have shown great potential in clinical applications. However, the cytotoxic effects of excessive Mg2. and the corrosion products from Mg-based biomaterials, particularly their effects on neurons, have been little studied. Although viability tests are most commonly used, a functional evaluation is critically needed. Here, both methyl thiazolyl tetrazolium （MTT） and lactate de- hydrogenase （LDH） assays were used to test the effect of Mg2. and Mg-extract solution on neuronal viability. microelectrode arrays （MEAs）, which provide long-term, real-time recording of extracellular electro- physiological signals of in vitro neuronal networks, were used to test for toxic effects. The minimum effective concentrations （ECmin） of Mg2. from the MTr and LDH assays were 3 mmol/L and 100 mmol/L respec- tively, while the ECmin obtained from the MEA assay was 0.1 mmol/L MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution, a concentra- tion that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons, MEA electrophysiological testing is a more precise method than basic cell-viability testing.

关键词： Magnesium microelectrode array Neuroelectrophysiology Neuron viability Neuronal network

microelectrode array recordings of excitability of low Mg^(2+)-induced acute hippocampal slices

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Neural Regeneration Research 2010年第20期5卷 1548-1551页

作者： Fan Yang Xinwei Gong Haiqing Gong Puming Zhang Peiji Liang Qinchi Lu Department of Neurology Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200001 China College of Life Science and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China

Neuronal connections can be detected by neuronal network discharges in hippocampal neurons cultured on ***,the multi-electrode-array（MEA）has not been widely used in hippocampal slice culture studies focused on *** present study induced spontaneous synchronous epileptiform activity using low Mg2＋artificial cerebrospinal fluid on acute hippocampal slices to record hippocampal discharges with *** showed that burst duration and average number of spikes in a burst were significantly greater in the CA3 compared with dentate gyrus and CA1 *** Schaffer cut-off group,CA1 area discharges disappeared,but synchronous discharges remained in the CA3 ***,synchronous discharge frequency in the Schaffer cut-off group was similar to ***,burst duration and average number of spikes in a burst were significantly decreased compared with control（P 〈 0.05）.Results demonstrated that highest neuronal excitability occurred in the CA3 area,and synchronous discharges induced by low Mg2＋originated from the CA3 region.

关键词： epilepsy low-Mg2+ hippocampus internal circuit-loop microelectrode array

A NEW MICRO-EDM REVERSE COPYING TECHNOLOGY FOR microelectrode array FABRICATION

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A NEW MICRO-EDM REVERSE COPYING TECHNOLOGY FOR MICROELECTROD...

Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays

2006年中国机械工程学会年会暨中国工程院机械与运载工程学部首届年会

作者： ZENG Weiliang*, WANG Zhenlong*, DONG Desheng* *School of Mechatronics Engineering, Harbin Institute of Technology, Harbin, China Department of Information Science, Harbin Normal University, Harbin, China

Micro electrical discharge machining (EDM), enhanced with ultrasonic vibration, is explored and assessed as a new technology for developing microelectrode array, for microelectrode array fabricated by

关键词： Micro-EDM microelectrode array reverse copying.

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Nano Research 2020年第6期13卷 1756-1763页

作者： Jingyu Xie Yilin Song Yuchuan Dai Ziyue Li Fei Gao Xuanyu Li Guihua Xiao Yu Zhang Hao Wang Zeying Lu Xingyu Jiang Wenfu Zheng Xinxia Cai State Key Laboratory of Transducer Technology Institute of ElectronicsChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences Beijing100049China Beijing Engineering Research Center for BioNanotechnology CAS Key Laboratory for Biological Effects of Nanomaterials and NanosafetyCAS Center for Excellence in NanoscienceNational Center for NanoScience and TechnologyBeijing100190China Department of Biomedical Engineering Southern University of Science and TechnologyShenzhen518055China

Effective and precise neural modulation with real-time detection in the brain is of great importance and represents a significant ***-encapsulated light-sensitive compounds have excellent characteristics such as high temporal and spatial resolution,delayed drug clearance,and restricted drug biodistribution for neural *** this study,we developed a nanoliposome-based delivery system for ruthenium-based caged GABA compounds(Nanolipo-Ru)to modulate neural activity and allow for real-time monitoring using the microelectrode arrays(MEAs).The Nanolipo-Ru nanoparticles had an average size of 134.10±4.30 nm and exhibited excellent stability for seven *** the in vivo experiment in the rat,release of GABA by Nanolipo-Ru under blue light illumination resulted in an average firing rate reduction in interneurons and pyramidal neurons in the same brain region of 79.4%and 81.6%,***,the average power of local field potentials in the 0–15 Hz range degraded from 4.34 to 0.85 *** addition,the Nanolipo-Ru nanoparticles have the potential to provide more flexible timing of modulation than unencapsulated RuBi-GABA in the *** results indicated that Nanolipo-Ru could be an effective platform for regulating neuronal ***,nanoliposomes with appropriate modifications would render promising utilities for targeting of specific types of neurons in the future.

关键词： neural modulation nanoliposome microelectrode array light-sensitive drug

Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms

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Journal of Bionic Engineering 2012年第1期9卷 1-10页

作者： Joose Kreutzer Laura Yla-Outinen Paula Karna Tiina Kaarela Jarno Mikkonen Heli Skottman Susanna Narkilahti Pasi KaUio Department of Automation Science and Engineering Tampere University of Technology Korkeakoulunkatu 3 33720 Tampere Finland BioMediTech Biokatu 10 33520 Tampere Finland NeuroGroup Institute of Biomedical Technology University of Tampere Biokatu 12 33520 Tampere Finland The Science Center of Pirkanmaa Hospital District Biokatu 12 33521 Tampere Finland Department of Psychology University of Jyvdskyld Ylistonmgientie 33 40500 Jyvdskyla Finland

Structured poly（dimethylsiloxane）（PDMS） chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell （hESC） - derived neuronal networks on microelectrode array （MEA） platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.

关键词： cell culturing electrical activity human embryonic stem cells microelectrode array poly(dimethylsiloxane)

Advances in electrical impedance tomography-based brain imaging

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Military Medical Research 2022年第6期9卷 705-726页

作者： Xi-Yang Ke Wei Hou Qi Huang Xue Hou Xue-Ying Bao Wei-Xuan Kong Cheng-Xiang Li Yu-Qi Qiu Si-Yi Hu Li-Hua Dong Department of Radiation Oncology and Therapy the First Hospital of Jilin UniversityChangchun 130021China Jilin Provincial Key Laboratory of Radiation Oncology and Therapy the First Hospital of Jilin UniversityChangchun 130021China NHC Key Laboratory of Radiobiology School of Public HealthJilin UniversityChangchun 130021China CAS Key Laboratory of Bio-Medical Diagnostics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhou 215163JiangsuChina

Novel advances in the field of brain imaging have enabled the unprecedented clinical application of various imaging modalities to facilitate disease diagnosis and treatment. Electrical impedance tomography(EIT) is a functional imaging technique that measures the transfer impedances between electrodes on the body surface to estimate the spatial distribution of electrical properties of tissues. EIT offers many advantages over other neuroimaging technologies,which has led to its potential clinical use. This qualitative review provides an overview of the basic principles,algorithms, and system composition of EIT. Recent advances in the field of EIT are discussed in the context of epilepsy,stroke, brain injuries and edema, and other brain diseases. Further, we summarize factors limiting the development of brain EIT and highlight prospects for the field. In epilepsy imaging, there have been advances in EIT imaging depth,from cortical to subcortical regions. In stroke research, a bedside EIT stroke monitoring system has been developed for clinical practice, and data support the role of EIT in multi-modal imaging for diagnosing stroke. Additionally, EIT has been applied to monitor the changes in brain water content associated with cerebral edema, enabling the early identification of brain edema and the evaluation of mannitol dehydration. However, anatomically realistic geometry,inhomogeneity, cranium completeness, anisotropy and skull type, etc., must be considered to improve the accuracy of EIT modeling. Thus, the further establishment of EIT as a mature and routine diagnostic technique will necessitate the accumulation of more supporting evidence.

关键词： Electrical impedance tomography(EIT) Brain diseases Tissue impedance microelectrode array

Highly-compliant,conformal and stretchable microelectrode arrays

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Engineering Sciences 2013年第3期11卷 72-75页

作者： Zhang Hongzhi Xie Lei Yu Mei Liu Zhiyuan Li Yuchun Yu Zhe Biomedical Microdevices Research Laboratory Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences Key Laboratory for Health Informatics Chinese Academy of Sciences

Most biological tissues are supple and elastic, while current electronic devices fabricated by semiconductors and metals are usually stiff and brittle. As a result, implanted electronic devices can irritate and damage surrounding tissues, causing immune reaction and scarring. In this work, we develop stretchable microelectrode arrays, with the development of a novel soft lithography technology, which are designed and fabricated with a polymer/stretchable metal/polymer sandwich structure. With the great deformability of stretch, compression, bend and twisting, while preserving electrical property, this technology overcomes the fundamental mismatch of mechanical properties between biological tissues and electronic devices, and provides highly-compliant, confonnal and stretchable bio-electronic interfaces. Here we also describe the following three applications of the stretchable electrode arrays： a. monitoring intracranial electroencephalography （EEG）; b. stimulating peripheral nerves to drive muscles; c. monitoring epicardial electrocardiography （ECG）. Stretchable microelectrode arrays create a promising field in biomedical applications for its better modulus match with biological tissues and robust mechanical and electrical properties. They allow for construction of electronic integrated circuits spread over on complex and dynamic curved surfaces, providing a much friendlier bio-electronic interface for diagnosis, treatment and in- telligent bio-control.

关键词： biological micro-electro-mechanical system (bioMEMS) microelectrode array EEG neural prosthesis ECG

Neural decoding based on probabilistic neural network

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第4期11卷 298-306页

作者： Yi YU Shao-min ZHANG Huai-jian ZHANG Xiao-chun LIU Qiao-sheng ZHANG Xiao-xiang ZHENG Jian-hua DAI Qiushi Academy for Advanced Studies Zhejiang University Hangzhou 310027 China College of Biomedical Engineering and Instrument Science Zhejiang University Hangzhou 31002 7 China Key Laboratory of Biomedical Engineering of Ministry of Education Zhejiang University Hangzhou 310027 China College of Computer Science and Technology Zhejiang University Hangzhou 310027 China

Brain-machine interface (BMI) has been developed due to its possibility to cure severe body paralysis. This technology has been used to realize the direct control of prosthetic devices,such as robot arms,computer cursors,and paralyzed muscles. A variety of neural decoding algorithms have been designed to explore relationships between neural activities and movements of the limbs. In this paper,two novel neural decoding methods based on probabilistic neural network (PNN) in rats were introduced,the PNN decoder and the modified PNN (MPNN) decoder. In the ex-periment,rats were trained to obtain water by pressing a lever over a pressure threshold. microelectrode array was implanted in the motor cortex to record neural activity,and pressure was recorded by a pressure sensor synchronously. After training,the pressure values were estimated from the neural signals by PNN and MPNN decoders. Their per-formances were evaluated by a correlation coefficient (CC) and a mean square error (MSE). The results show that the MPNN decoder,with a CC of 0.8657 and an MSE of 0.2563,outperformed the traditionally-used Wiener filter (WF) and Kalman filter (KF) decoders. It was also observed that the discretization level did not affect the MPNN performance,indicating that the MPNN decoder can handle different tasks in BMI system,including the detection of movement states and estimation of continuous kinematic parameters.

关键词： Brain-machine interfaces (BMI) Neural decoding Probabilistic neural network (PNN) microelectrode array

Three-dimensional fuzzy graphene ultra-microelectrodes for subcellular electrical recordings

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Nano Research 2020年第5期13卷 1444-1452页

作者： Sahil K.Rastogi Jacqueline Bliley Laura Matino Raghav Garg Francesca Santoro Adam W.Feinberg Tzahi Cohen-Karni Department of Biomedical Engineering Carnegie Mellon UniversityPittsburghPA15213USA Center for Advanced Biomaterials for Healthcare Istituto Italiano di TecnologiaNaples80125Italy Department of Chemical Materials and Industrial Production EngineeringUniversity of Naples Federico IINaples80125Italy Department of Materials Science and Engineering Carnegie Mellon UniversityPittsburghPA15213USA

microelectrode arrays(MEAs)have enabled investigation of cellular networks at sub-millisecond temporal ***,current MEAs are limited by the large electrode footprint since reducing the electrode’s geometric area to sub-cellular dimensions leads to a significant increase in impedance thus affecting its recording *** report a breakthrough ultra-microelectrodes platform by leveraging the outstanding surface-to-volume ratio of nanowire-templated out-of-plane synthesized three-dimensional fuzzy graphene(NT-3DFG).The enormous surface area of NT-3DFG leads to 140-fold reduction in electrode impedance compared to bare Au microelectrodes,thus enabling scaling down the geometric size by 625-fold to ca.2µm×2µ*** out-of-plane morphology of NT-3DFG leads to a tight seal with the cell membrane thus enabling recording of electrical signals with high signal-to-noise ratio(SNR)of>*** work highlights the possibility to push the limits of the conventional MEA technology to enable electrophysiological investigation at sub-cellular level without the need of any surface *** presented approach would greatly impact our basic understanding of signal transduction within a single cell as well as complex cellular assemblies.

关键词： microelectrode array ultra-microelectrodes electrical recordings biocompatible hybrid-nanomaterial 3D graphene cardiomyocytes