Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial

作     者：Ting Huang ZhonghaiWang Lina Wei Mark Kindy Yufeng Zheng Tingfei Xi Bruce Z. Gao 

作者机构：Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China Department of Bioengineering Clemson University Clemson SC29634 USA Departments of Neurosciences and Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston SC29466 USA Ralph H. Johnson VA Medical Center Charleston SC29403 USA Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China Shenzhen Key Laboratory of Human Tissue Regeneration and Repair Shenzhen Institute Peking University Shenzhen 518057 China 

出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)

年 卷 期：2016年第32卷第1期

页      面：89-96页

核心收录：

学科分类：08[工学] 080501[工学-材料物理与化学] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：supported by the National Basic Research Program of China (973 Program,No.2012CB619102) the National Natural Science Foundation of China (Nos.31070847 and 31370956) the National Science and Technology Support Program (No.2012BAI18B01) the Strategic New Industry Development Special Foundation of Shenzhen,China (No.JCYJ20130402172114948) the Guangdong Provincial Department of Science and Technology,China (No.2011B050400011) NIH NIGMS COBRE (No.NIH P20GM103444) 

主　　题：Magnesium Microelectrode array Neuroelectrophysiology Neuron viability Neuronal network 

摘      要：Magnesium （Mg）-based biomaterials have shown great potential in clinical applications. However, the cytotoxic effects of excessive Mg2. and the corrosion products from Mg-based biomaterials, particularly their effects on neurons, have been little studied. Although viability tests are most commonly used, a functional evaluation is critically needed. Here, both methyl thiazolyl tetrazolium （MTT） and lactate de- hydrogenase （LDH） assays were used to test the effect of Mg2. and Mg-extract solution on neuronal viability. Microelectrode arrays （MEAs）, which provide long-term, real-time recording of extracellular electro- physiological signals of in vitro neuronal networks, were used to test for toxic effects. The minimum effective concentrations （ECmin） of Mg2. from the MTr and LDH assays were 3 mmol/L and 100 mmol/L respec- tively, while the ECmin obtained from the MEA assay was 0.1 mmol/L MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution, a concentra- tion that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons, MEA electrophysiological testing is a more precise method than basic cell-viability testing.