Microelectrode array recordings of excitability of low Mg^(2+)-induced acute hippocampal slices

作     者：Fan Yang Xinwei Gong Haiqing Gong Puming Zhang Peiji Liang Qinchi Lu 

作者机构：Department of Neurology Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200001 China College of Life Science and Biotechnology Shanghai Jiao Tong University Shanghai 200240 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2010年第5卷第20期

页      面：1548-1551页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 080202[工学-机械电子工程] 08[工学] 071006[理学-神经生物学] 0802[工学-机械工程] 

主　　题：epilepsy low-Mg2+ hippocampus internal circuit-loop microelectrode array 

摘      要：Neuronal connections can be detected by neuronal network discharges in hippocampal neurons cultured on ***,the multi-electrode-array（MEA）has not been widely used in hippocampal slice culture studies focused on *** present study induced spontaneous synchronous epileptiform activity using low Mg2＋artificial cerebrospinal fluid on acute hippocampal slices to record hippocampal discharges with *** showed that burst duration and average number of spikes in a burst were significantly greater in the CA3 compared with dentate gyrus and CA1 *** Schaffer cut-off group,CA1 area discharges disappeared,but synchronous discharges remained in the CA3 ***,synchronous discharge frequency in the Schaffer cut-off group was similar to ***,burst duration and average number of spikes in a burst were significantly decreased compared with control（P 〈 0.05）.Results demonstrated that highest neuronal excitability occurred in the CA3 area,and synchronous discharges induced by low Mg2＋originated from the CA3 region.