Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms

作     者：Joose Kreutzer Laura Yla-Outinen Paula Karna Tiina Kaarela Jarno Mikkonen Heli Skottman Susanna Narkilahti Pasi KaUio 

作者机构：Department of Automation Science and Engineering Tampere University of Technology Korkeakoulunkatu 3 33720 Tampere Finland BioMediTech Biokatu 10 33520 Tampere Finland NeuroGroup Institute of Biomedical Technology University of Tampere Biokatu 12 33520 Tampere Finland The Science Center of Pirkanmaa Hospital District Biokatu 12 33521 Tampere Finland Department of Psychology University of Jyvdskyld Ylistonmgientie 33 40500 Jyvdskyla Finland 

出 版 物：《Journal of Bionic Engineering》 (仿生工程学报（英文版）)

年 卷 期：2012年第9卷第1期

页      面：1-10页

核心收录：

学科分类：12[管理学] 081704[工学-应用化学] 07[理学] 08[工学] 070305[理学-高分子化学与物理] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1201[管理学-管理科学与工程(可授管理学、工学学位)] 081104[工学-模式识别与智能系统] 0817[工学-化学工程与技术] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0835[工学-软件工程] 0703[理学-化学] 0836[工学-生物工程] 0811[工学-控制科学与工程] 0702[理学-物理学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：The Competitive Research Funding of the Tampere University Hospital 

主　　题：cell culturing electrical activity human embryonic stem cells microelectrode array poly(dimethylsiloxane) 

摘      要：Structured poly（dimethylsiloxane） （PDMS） chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell （hESC） - derived neuronal networks on Microelectrode Array （MEA） platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.