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Sublethal exposure to spinetoram impacts life history traits and dengue virus replication in Aedes aegypti

Insect Science 2023年第2期30卷 486-500页

作者： Luoluo Wang Zhichang Pang Qi Chen Zhentao Song Yi Lu Meng Yang Jia Huang Xiao-Qiang Yu Xiaoyun Wang Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Instute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhouChina

Insecticides are anthropogenic environmental stressors and also a common stressor for mosquito vectors.However,the use of insecticides is often guided by short-term efficacy,and the sublethal effect on their target or nontarget species has long been ignored.Here,we analyzed how sublethal exposure of the promising vector-control bioinsecticide spinetoram to Aedes aegypti larvae alter adult performance and susceptibility to dengue virus(DENV)infection.We found that the surviving adult mosquitoes were significantly smaller and exhibited weaker blood-feeding capacity than control females,apart from the extended immature development period.In terms of reproductive potential,although the F_(0) generation produced a similar number of eggs and offspring during the first gonotrophic cycle,the survival rates of the F_(1) generations were significantly lower as compared to the control group,suggesting transgenerational sublethal effects on the F_(1) generation.Notably,surviving adult females had higher DENV-2 viral loads than the control group after spinetoram sublethal exposure.Mechanistically,transcriptomic analysis showed that inhibition of oxidative phosphorylation may function in stimulating DENV production in adult Ae.aegypti.In Aag2 cells,significant accumulation of apoptosis,mitochondrial reactive oxygen species production,and DENV-2 replication by spinetoram exposure consistently support our conclusion.Our study highlights the threat of sublethal spinetoram exposure on outbreaks of mosquito-borne viruses.

关键词： Aedes aegypti dengue virus spinetoram sublethal exposure susceptibility

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Surveillance of arthropod-borne viruses in Benin,West Africa 2020–2021:detection of dengue virus 3 in Aedes aegypti(Diptera:Culicidae)

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Military Medical Research 2023年第5期10卷 730-732页

作者： Carine Tchibozo Gildas Hounkanrin Anges Yadouleton Alexandra Bialonski Eric Agboli Renke Luhken Jonas Schmidt‑Chanasit Hanna Jost Laboratory of Viral Haemorrhagic Fevers and Arboviruses of Benin 01BP 918CotonouBenin Centre de Recherche Entomologique de Cotonou 01BP 918CotonouBenin Ecole Normale Supérieure de Natitingou National Universityof ScienceTechnologyEngineering and MathematicsAbomeyBenin Bernhard Nocht Institute for Tropical Medicine WHO Collaborating Centrefor Arbovirus and Haemorrhagic Fever Reference and Research20359 Ham‑burgGermany University of Health and Allied Sciences PMB 31HoGhana Faculty of Mathematics Informatics and Natural SciencesUniversitat Hamburg22609 HamburgGermany

Dear Editor,Dengue virus(DENV,family Flaviviridae,genus Flavivirus)serotypes 1 to 4(DENV-1,-2,-3,and-4)are responsible for more than 100 million infections per year worldwide.Symptoms of DENV infection can be diverse,reaching from an acute febrile illness to the more severe,sometimes fatal dengue haemorrhagic fever/dengue shock syndrome.

关键词： Dengue virus Arbovirus Mosquitoes Aedes aegypti Benin

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Modelling the probability of presence of Aedes aegypti and aedes albopictus in Iran until 2070

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Asian Pacific Journal of Tropical Medicine 2023年第1期16卷 16-25页

作者： Mohammad Mehdi Sedaghat Faramarz Bozorg Omid Mohammad Karimi Sajjad Haghi Ahmad Ali Hanafi-Bojd Department of Vector Biology&Control School of Public HealthTehran University of Medical SciencesTehranIran Department of GIS Faculty of Geodesy and Geomatics EngineeringK.N.Toosi University of TechnologyTehranIran Zoonoses Research Center Tehran University of Medical SciencesTehranIran

Objective:To determine the suitable ecological habitats of aedes(Ae.)aegypti and Ae.albopictus in Iran due to climate change by the 2070s.Methods:All data relating to the spatial distribution of Ae.aegypti and Ae.albopictus worldwide,which indicated the geographical coordinates of the collection sites of these mosquitoes,were extracted from online scientific websites and entered into an Excel file.The effect of climatic and environmental variables on these mosquitoes was evaluated using the MaxEnt model in the current and future climatic conditions in the 2030s,2050s,and 2070s.Results:The most suitable areas for the establishment of Ae.aegypti are located in the southern and northern coastal areas of Iran,based on the model outputs.The modelling result for suitable ecological niches of Ae.albopictus shows that in the current climatic conditions,the southern half of Iran from east to west,and parts of the northern coasts are prone to the presence of this species.In the future,some regions,such as Gilan and Golestan provinces,will have more potential to exist/establish Ae.albopictus.Also,according to the different climate change scenarios,suitable habitats for this species will gradually change to the northwest and west of the country.The temperature of the wettest season of the year(Bio8)and average annual temperature(Bio1)were the most effective factors in predicting the model for Ae.aegypti and Ae.albopictus,respectively.Conclusions:It is required to focus on entomological studies using different collection methods in the vulnerable areas of Iran.The future modelling results can also be used for long-term planning to prevent the entry and establishment of these invasive aedes vectors in the country.

关键词： Aedes aegypti aedes albopictus Dengue fever Chikungunya Ecological Niche Modeling Climate change

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Evaluation of Cuban Bacillus thuringiensis(Berliner,1911)(Bacillales:Bacillacea)isolates with larvicidal activity against Aedes aegypti(Linnaeus,1762)(Diptera:Culicidae)

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Asian Pacific Journal of Tropical Medicine 2022年第2期15卷 79-86页

作者： Aileen Gonzalez Rizo Camilo E Castañet Martinez Celeste Ramírez Cardentey Ariamys Companioni Ibañez Zulema Menéndez Díaz Lianet Monzote Fidalgo Hilda M HernandezÁlvarez Vector Control Department Institute of Tropical MedicinePedro KouríAutopistaNovia del Mediodía km 6½La Habana 11400Cuba Virology Department Institute of Tropical MedicinePedro KouríAutopista Novia del Mediodía km 6½La Habana 11400Cuba Parasitology Department Institute of Tropical MedicinePedro KouríAutopista Novia del Mediodía km 6½La Habana 11400Cuba

Objective:To evaluate 11 Cuban native Bacillus(B.)thuringiensis isolates in order to select one with the best larvicidal activity against aedes(Ae.)aegypti and low cytotoxicity.Methods:The cry and cyt genes of the isolates(A21,A51,L95,L910,M29,R84,R85,R87,R89,U81 and X48)were amplified by PCR.The influence of organic matter and NaCl on the larvicidal activity was tested by bioassays.Cytotoxicity was assayed on peritoneal macrophages of BALB/c mice.Results:The cyt1(Aa,Ab,Ba),cyt2,cry4aA,cry4Ba,cry11(Aa,Ba,Bb)and cry10 genes were identified in all native Cuban isolates.The larvicidal activity(LC_(90))of seven isolates was affected by the presence of organic matter in the water,while A21,A51,L910,R84,U81 and X48 had better LC_(50),LC_(90),LC_(95) than the 266/29-Ⅶ-98 control strain.The LC_(50) of two isolates was affected by the presence of NaCl and A21,A51,R85 isolate had better larvicidal activity than the 266/29-Ⅶ-98 control strain.In terms of toxicity against macrophages,the extracts of nine isolates were less cytotoxic than the control strains.Conclusions:Native isolate A21 had the main virulence factors against Ae.aegypti larvae,displayed a good larvicidal activity in presence of different factors related with Ae.aegypti breeding sites,and had low citotoxicity against macrophages.These results can contribute to the improvement of existing biological control strategies and the development of new biolarvicides.

关键词： Mosquitoes Biological control agent Bacillus thuringiensis Bioassays Aedes aegypti cry and cyt genes

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Larvicidal activity of the pericarp extract of Garcinia mangostana against dengue vector Aedes aegypti in Sri Lanka

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Asian Pacific Journal of Tropical Medicine 2022年第3期15卷 135-142页

作者： Nayana Gunathilaka Yashoda Wijebandara Deepika Amerasinghe Lahiru Udayanga Thusitha PMuhandiramlage Department of Parasitology Faculty of MedicineUniversity of KelaniyaRagamaSri Lanka Department of Zoology and Environmental Management Faculty of ScienceUniversity of KelaniyaDalugamaSri Lanka Department of Biosystems Engineering Faculty of Agriculture and Plantation ManagementWayamba University of Sri LankaMakaduraSri Lanka Department of Biochemistry and Clinical Chemistry Faculty of MedicineUniversity of KelaniyaRagamaSri Lanka

Objective:To assess the larvicidal activity of mangosteen(Garcinia mangostana)against larval stages of Aedes aegypti mosquitoes.Methods:A crude extract was prepared in ethanol from powdered mangosteen pericarps.A concentration gradient(0.01-4.92 g/L)was prepared from the stock solution.Seven batches of 25 third instar larvae of Aedes aegypti were used for larval bioassays.Larval mortality rates were observed after one and 24 hours.Cholesterol and total lipid contents in 20 randomly selected dead larvae at each trial were assessed by colorimetric method.The experimental setup was repeated five times.The General Linear Model and Probit analysis were used to evaluate the relationship of mortality with cholesterol level,total lipid level and cholesterol to total lipid ratio.Results:The percentage mortalities significantly varied with different concentrations(F_(7,32)=385.737;PAedes aegypti mosquitoes.Hence,it could be explored for use as a key source for the development of an environment-friendly plant-based larvicide.

关键词： Mangosteen Mosquito Larvicide Garcinia mangostana Aedes aegypti

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The effect of bacterial challenge on ferritin regulation in the yellow fever mosquito, Aedes aegypti

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Insect Science 2013年第5期20卷 601-619页

作者： Dawn L. Geiser Guoli Zhou Jonathan J. Mayo Joy J. Winzerling Department of Nutritional Sciences College of Agriculture and Life Sciences the University of Arizona Tucson Arizona 85721 USA

Secreted ferritin is the major iron storage and transport protein in insects. Here, we characterize the message and protein expression profiles of yellow fever mosquito （Aedes aegypti） ferritin heavy chain homologue （HCH） and light chain homologue （LCH） subunits in response to iron and bacterial challenge. In vivo experiments demonstrated tissue-specific regulation of HCH and LCH expression over time post-blood meal （PBM）. Transcriptional regulation of HCH and LCH was treatment specific, with differences in regulation for na？ve versus mosquitoes challenged with heat-killed bacteria （HKB）. Translational regulation by iron regulatory protein （IRP） binding activity for the iron-responsive element （IRE） was tissue-specific and time-dependent PBM. However, mosquitoes challenged with HKB showed little change in IRP/IRE binding activity compared to na？ve animals. The changes in ferritin regulation and expression in vivo were confirmed with in vitro studies. We challenged mosquitoes with HKB followed by a blood meal to determine the effects on ferritin expression, and demonstrate a synergistic, time-dependent regulation of expression for HCH and LCH.

关键词： Aedes aegypti bacteria ferritin iron IRP mosquito

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Arylalkalamine N-acetyltransferase-1 functions on cuticle pigmentation in the yellow fever mosquito,Aedes aegypti

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Insect Science 2021年第6期28卷 1591-1600页

作者： Lei Zhang Miao-Zhen Li Zhao-Hui Chen Yu Tang Cheng-Hong Liao Qian Han Laboratory of Tropical Veterinary Medicine and Vector Biology School of Life and Pharmaceutical SciencesHainan UniversityHaikou570228China

Arylalkylamine N-acetyltransferase(aaNAT)catalyzes the acetylation of dopamine,5-hydroxy-tryptamine,tryptamine,octopamine,norepinephrine and other ary-lalkylamines to form respective N-acetyl-arylalkylamines.Depending on the products formed,aaNATs are involved in a variety of physiological functions.In the yellow fever mosquito,Aedes aegypti,a number of aaNATs and aaNAT-like proteins have been reported.However,the primary function of each individual aaNAT is yet to be identified.In this study we investigated the function of Ae.aegypti aaNAT 1(Ae-aaNATl)in cuticle pigmentation and development of morphology.Ae-aaNAT1 transcripts were detected at all stages of development with highest expressions after pupation and right before adult eclosion.Ae-aaNATl mutant mosquitoes generated using clustered regularly interspaced palindromic repeats(CRISPR)-CRISPR-associated protein 9 had no obvious effect on larval and pupal development.However,the mutant mosquitoes exhibited a roughened ex-oskeletal surface,darker cuticles,and color pattern changes suggesting that Ae-aaNAT1 plays a role in development of the morphology and pigmentation of Ae.aegypti adult cuticles.The mutant also showed less blood feeding efficiency and lower fecundity when compared with the wild-type.The mutation of Ae-aaNAT1 influenced expression of genes involved in cuticle formation.In summary,Ae-aaNAT1 mainly functions on cuticular pigmentation and also affects blood feeding efficiency and fecundity.

关键词： arylalkylamine Aedes aegypti CRISPR-Cas9 cuticle pigmentation gene expression N-acetyltransferase

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Habitat productivity and pyrethroid susceptibility status of Aedes aegypti mosquitoes in Dar es Salaam,Tanzania

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Infectious Diseases of Poverty 2017年第1期6卷 921-930页

作者： Leah Mathias Vito Baraka Anitha Philbert Ester Innocent Filbert Francis Gamba N.kwengulila Eliningaya J.Kweka Department of Zoology and Wildlife Conservation College of Natural and Applied SciencesUniversity of Dar es SalaamP.O.Box 35165Dar es SalaamTanzania National Institute for Medical Research Tanga Medical Research CentreP.O.Box 5004Tanga UrbanTangaTanzania Global Health Institute University of AntwerpGouverneur KinsbergencentrumDoornstraat 331B-2610WilrijkBelgium Mkwawa University College of Education Private BagIringaTanzania Institute of Traditional Medicine Muhimbili University of Health and Allied SciencesP.O.Box 65001Dar es SalaamTanzania Department of Medical Parasitology and Entomology Catholic University of Health and Allied SciencesP.O.Box 1464MwanzaTanzania Division of Livestock and Human Diseases Vector Control Tropical Pesticides Research InstituteNgaramtoniOff Nairobi RoadP.O.Box 3024ArushaTanzania

Background:Aedes aegypti(Diptera:Culicidae)is the main vector of the dengue virus globally.Dengue vector control is mainly based on reducing the vector population through interventions,which target potential breeding sites.However,in Tanzania,little is known about this vector’s habitat productivity and insecticide susceptibility status to support evidence-based implementation of control measures.The present study aimed at assessing the productivity and susceptibility status of A.aegypti mosquitoes to pyrethroid-based insecticides in Dar es Salaam,Tanzania.Methods:An entomological assessment was conducted between January and July 2015 in six randomly selected wards in Dar es Salaam,Tanzania.Habitat productivity was determined by the number of female adult A.aegypti mosquitoes emerged per square metre.The susceptibility status of adult A.aegypti females after exposure to 0.05%deltamethrin,0.75%permethrin and 0.05%lambda-cyhalothrin was evaluated using the standard WHO protocols.Mortality rates were recorded after 24 h exposure and the knockdown effect was recorded at the time points of 10,15,20,30,40,50 and 60 min to calculate the median knockdown times(KDT50 and KDT95).Results:The results suggest that disposed tyres had the highest productivity,while water storage tanks had the lowest productivity among the breeding habitats Of A.aegypti mosquitoes.All sites demonstrated reduced susceptibility to deltamethrin(0.05%)within 24 h post exposure,with mortalities ranging from 86.3±1.9(mean±SD)to 96.8±0.9(mean±SD).The lowest and highest susceptibilities were recorded in Mikocheni and Sinza wards,respectively.Similarly,all sites demonstrated reduced susceptibility permethrin(0.75%)ranging from 83.1±2.1%(mean±SD)to 96.2±0.9%(mean±SD),in Kipawa and Sinza,respectively.Relatively low mortality rates were observed in relation to lambda-cyhalothrin(0.05%)at all sites,ranging from 83.1±0.7(mean±SD)to 86.3±1.4(mean±SD).The median KDT_(50)for deltamethrin,permethrin and lambda-cyhalothrin were 24.9-30.3 min,24.3-34.4 min and 26.7-

关键词： Culicidae Aedes aegypti Abundance Productivity Pyrethroid Knockdown effect Dar es Salaam Tanzania

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First detection of F1534C knockdown resistance mutation in Aedes aegypti(Diptera:Culicidae)from Cameroon

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Infectious Diseases of Poverty 2020年第6期9卷 51-62页

作者： Aurelie P.Yougang Basile Kamgang Theodel A.Wilson Bahun Armel N.Tedjou Daniel Nguiffo-Nguete Flobert Njiokou Charles S.Wondji Centre for Research in Infectious Diseases P.O.Box 13591YaoundéCameroon Parasitology and Ecology Laboratory Department of Animal Biology and PhysiologyFaculty of ScienceUniversity of Yaoundé1P.O.Box 812YaoundéCameroon Laboratory of Vertebrate and Invertebrate Bioecology Faculty of Science and TechnologyMarien-Ngouabi UniversityBrazzavilleCongo Laboratory of Biology and Applied Ecology Department of Animal BiologyFaculty of ScienceUniversity of DschangP.O.Box 96DschangCameroon Liverpool School of Tropical Medicine Pembroke placeLiverpoolL35QAUK

Background: aedes borne viral diseases,notably dengue,are increasingly reported in Cameroon with Aedes aegypti being a major vector.Data on insecticide resistance of this vector and underlying mechanisms needed for outbreak preparedness remain scarce in Cameroon.Here,we present the nationwide distribution of insecticide resistance in Ae.aegypti and investigate the potential resistance mechanisms involved.Methods:: Immature stages of Ae.aegypti were collected between March and July 2017 in 13 locations across Cameroon and reared until G1/G2/G3 generation.Larval,adult bioassays,and piperonyl butoxide(PBO)synergist assays were carried out according to World Health Organization guidelines.F1534C mutation was genotyped using allele specific polymerase chain reaction in field collected adults(Go)and the polymorphism of the sodium channel gene was assessed.Theχ2 test was used to compare the mortality rate between bioassays with insecticides only and bioassays after preexposure to PBO synergist.Results: Larval bioassay revealed that all the three populations tested with temephos were susceptible.Adult bioassays showed a good level of susceptibility toward both pyrethroids tested,0.25%permethrin and 0.05%deltamethrin,with six out of 10 populations susceptible.However,two populations(Douala and Edéa)were resistant(deltamethrin[73.2–92.5%mortality],permethrin[2.6–76.3%mortality]).The resistance to 4%dichlorodiphenyltrichloroethane was observed in four out of 10 populations tested(16.8–87.1%mortality).Resistance was also reported to carbamates including 0.1%propoxur(60.8–87.1%mortality)and to 0.1%bendiocarb(82.9%mortality).All populations tested were fully susceptible to 1%fenitrothion.A partial recovery of susceptibility was observed in the pyrethroid resistant population of Douala after pre-exposed to PBO suggesting the implication of cytochrome P450 monoxygenases permethrin resistance.Genotyping and sequencing detected the F1534C kdr mutation in the two pyrethroid resistant locations of Edéa and Douala,with allelic freque

关键词： Aedes aegypti Insecticide resistance Kdr mutation Cameroon Arbovirus

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Levels of insecticide resistance to deltamethrin,malathion,and temephos,and associated mechanisms in Aedes aegypti mosquitoes from the Guadeloupe and Saint Martin islands(French West Indies)

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Infectious Diseases of Poverty 2017年第1期6卷 339-353页

作者： Daniella Goindin Christelle Delannay Andric Gelasse Cedric Ramdini Thierry Gaude Frederic Faucon Jean-Philippe David Joël Gustave Anubis Vega-Rua Florence Fouque Laboratory of Medical Entomology Unit Environment and HealthPasteur Institute of Guadeloupe97183 Les AbymesGuadeloupe Vector control Service Regional Health AgencyDothemareLes AbymesGuadeloupe Alpine Ecology Laboratory(LECA) CNRSUMR 55532233 rue de la piscine BP5338041 GrenobleCedex 9France University of Grenoble Alpes GrenobleFrance Environmental and Systems Biology(BEeSy) University of Grenoble AlpesGrenobleFrance Vector Environment and Society Unit Special Programme for Research and Training in Tropical Diseases(TDR)World Health Organization20Avenue AppiaCH-1211 Geneva 27Switzerland

Background:In the Guadeloupe and Saint Martin islands,Aedes aegypti mosquitoes are the only recognized vectors of dengue,chikungunya,and Zika viruses.For around 40 years,malathion was used as a mosquito adulticide and temephos as a larvicide.Since the European Union banned the use of these two insecticide molecules in the first decade of the 21st century,deltamethrin and Bacillus thuringiensis var.israelensis are the remaining adulticide and larvicide,respectively,used in Guadeloupe.In order to improve the management of vector control activities in Guadeloupe and Saint Martin,we investigated Ae.aegypti resistance to and mechanisms associated with deltamethrin,malathion,and temephos.Methods:Ae.aegypti mosquitoes were collected from six different localities of Guadeloupe and Saint Martin.Larvae were used for malathion and temephos bioassays,and adult mosquitoes for deltamethrin bioassays,following World Health Organization recommendations.Knockdown resistance(Kdr)genotyping for V1016I and F1534C mutations,and expression levels of eight enzymes involved in detoxification mechanisms were examined in comparison with the susceptible reference Bora Bora strain.Results:Resistance ratios(RR50)calculated for Ae.aegypti larvae showed high resistance levels to temephos(from 8.9 to 33.1-fold)and low resistance levels to malathion(from 1.7 to 4.4-fold).Adult females displayed moderate resistance levels to deltamethrin regarding the time necessary to affect 50%of individuals,varying from 8.0 to 28.1-fold.Molecular investigations on adult mosquitoes showed high resistant allele frequencies for V1016I and F1534C(from 85 to 96%and from 90 to 98%,respectively),as well as an overexpression of the glutathione S-transferase gene,GSTe2,the carboxylesterase CCEae3a,and the cytochrome genes 014614,CYP6BB2,CYP6M11,and CYP9J23.Conclusions:Ae.aegypti populations from Guadeloupe and Saint Martin exhibit multiple resistance to organophosphates(temephos and malathion),and pyrethroids(deltamethrin).The mechanisms associated with these resistanc

关键词： Aedes aegypti Mosquitoes Insecticide resistance Deltamethrin Malathion Temephos Guadeloupe Saint Martin

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