Evaluation of Cuban Bacillus thuringiensis(Berliner,1911)(Bacillales:Bacillacea)isolates with larvicidal activity against Aedes aegypti(Linnaeus,1762)(Diptera:Culicidae)

作     者：Aileen Gonzalez Rizo Camilo E Castañet Martinez Celeste Ramírez Cardentey Ariamys Companioni Ibañez Zulema Menéndez Díaz Lianet Monzote Fidalgo Hilda M HernandezÁlvarez Aileen González Rizo;Camilo E Casta?et Martinez;Celeste Ramírez Cardentey;Ariamys Companioni Iba?ez;Zulema Menéndez Díaz;Lianet Monzote Fidalgo;Hilda M Hernandez (á)lvarez

作者机构：Vector Control DepartmentInstitute of Tropical MedicinePedro KouríAutopistaNovia del Mediodía km 6½La Habana 11400Cuba Virology DepartmentInstitute of Tropical MedicinePedro KouríAutopista Novia del Mediodía km 6½La Habana 11400Cuba Parasitology DepartmentInstitute of Tropical MedicinePedro KouríAutopista Novia del Mediodía km 6½La Habana 11400Cuba 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2022年第15卷第2期

页      面：79-86页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

主　　题：Mosquitoes Biological control agent Bacillus thuringiensis Bioassays Aedes aegypti cry and cyt genes 

摘      要：Objective:To evaluate 11 Cuban native Bacillus(B.)thuringiensis isolates in order to select one with the best larvicidal activity against Aedes(Ae.)aegypti and low ***:The cry and cyt genes of the isolates(A21,A51,L95,L910,M29,R84,R85,R87,R89,U81 and X48)were amplified by *** influence of organic matter and NaCl on the larvicidal activity was tested by *** was assayed on peritoneal macrophages of BALB/c ***:The cyt1(Aa,Ab,Ba),cyt2,cry4aA,cry4Ba,cry11(Aa,Ba,Bb)and cry10 genes were identified in all native Cuban *** larvicidal activity(LC_(90))of seven isolates was affected by the presence of organic matter in the water,while A21,A51,L910,R84,U81 and X48 had better LC_(50),LC_(90),LC_(95) than the 266/29-Ⅶ-98 control *** LC_(50) of two isolates was affected by the presence of NaCl and A21,A51,R85 isolate had better larvicidal activity than the 266/29-Ⅶ-98 control *** terms of toxicity against macrophages,the extracts of nine isolates were less cytotoxic than the control ***:Native isolate A21 had the main virulence factors against *** larvae,displayed a good larvicidal activity in presence of different factors related with *** breeding sites,and had low citotoxicity against *** results can contribute to the improvement of existing biological control strategies and the development of new biolarvicides.