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Chinese Journal of Chemical Engineering 2023年第1期53卷 101-123页

作者： Wenjuan Yan Puhua Sun Chen Luo Xingfan Xia zhifei Liu Yuming zhao Shuxia Zhang Liang Sun Feng Du State Key Laboratory of Heavy Oil Processing Center for Chemical Engineering Experimental TeachingOil Industry Training CenterChina University of PetroleumQingdao 266580China Petrochemical Research Institute PetroChinaBeijing 102200China

Oxygen reduction reaction over Pt-based catalyst is one of the most significant cathode reactions in fuel ***,low reserves and high price of Pt have motivated researchers worldwide seeking enhanced utilization efficiency and durability by doping non-noble metals to form Pt-based alloy *** Pt with Co has been recognized as one of the most effective approaches to achieve this *** bimetal combination is one of the most promising candidates to synthesize highly efficient catalysts for oxygen reduction reaction(ORR)applications,owing to its relatively more suitable oxygen binding energy for four-electron transfer ***,impressive strategies have been developed to fabricate more active and stable PtCo-based multimetallic alloys with tailorable size and *** paper aims to summarize the most recent highlights on the study of the relationship between preparation strategies,morphologies,electroactivities of the PtCo-based catalyst at atomic level and further the relevant reaction *** challenges and opportunities on the further development of electrocatalysts for fuel cells are included to provide reference for the practical application.

关键词： Electrochemistry Catalysis Oxygen reduction reaction Fuel cells PtCo nanoalloy Multimetallic

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超厚层4H-SiC同质外延材料

固体电子学研究与进展 2019年第2期39卷 F0003-F0003页

作者：李赟李忠辉赵志飞王翼周平南京电子器件研究所微波毫米波单片集成和模块电路重点实验室南京210016

缺陷控制是4H-SiC同质外延的关键技术。在所有的4H-SiC外延缺陷中,三角形缺陷对芯片性能的影响最为致命,而且三角形缺陷的尺寸随着4H-SiC外延层厚度的增加迅速增大,从而会导致外延片无缺陷面积急剧下降。因此对于厚层4H-SiC外延,控制三... 详细信息

缺陷控制是4H-SiC同质外延的关键技术。在所有的4H-SiC外延缺陷中,三角形缺陷对芯片性能的影响最为致命,而且三角形缺陷的尺寸随着4H-SiC外延层厚度的增加迅速增大,从而会导致外延片无缺陷面积急剧下降。因此对于厚层4H-SiC外延,控制三角形缺陷密度至关重要。南京电子器件研究所追踪衬底/外延层界面处的缺陷转化信息,分析了三角形缺陷的主要成因,并通过优化衬底刻蚀时间及温度,有效消除由于衬底表面加工损伤引入的三角形成核几率。

关键词： 4H-SiC 外延材料厚层南京电子器件研究所缺陷控制三角形同质外延芯片性能

CRISPR/Cas9基因编辑系统应用于非人灵长类细胞及胚胎Rb1基因编辑的研究

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解放军医学院学报 2018年第8期39卷 707-712页

作者：怀思远褚楚曹静赵志飞吴璇冀天楠李建雄解放军总医院放射治疗科北京100853 云南中科灵长类生物医学重点实验室云南昆明650500

目的探索聚簇规则间隔短回文重复序列/CRISPR相关核酸酶9(CRISPR/Cas9)基因编辑系统对于食蟹猴细胞及胚胎基因组视网膜母细胞瘤抑癌基因(Rb1)的编辑效率,旨在进一步建立视网膜母细胞瘤模型。方法查阅RB1突变数据库(http://***),确定视... 详细信息

目的探索聚簇规则间隔短回文重复序列/CRISPR相关核酸酶9(CRISPR/Cas9)基因编辑系统对于食蟹猴细胞及胚胎基因组视网膜母细胞瘤抑癌基因(Rb1)的编辑效率,旨在进一步建立视网膜母细胞瘤模型。方法查阅RB1突变数据库(http://***),确定视母细胞瘤发生发展相关Rb1基因外显子区主要有8号外显子;利用DNAman检测人类与食蟹猴基因组中Rb1基因中与是否存在单核苷酸多态性(SNP);通过http://***/***设计并合成sgRNA;采用CRISPR/Cas9基因编辑系统转染食蟹猴成纤维细胞,并提取转染后细胞基因组以检测合成的sgRNA敲除效率;酶切实验检测转染的细胞是否发生突变,并利用单克隆分析测定具体敲除效率。进一步利用显微操作技术注射sgRNA和Cas9 nuclease混合物(RNP)入单细胞胚胎,检测胚胎水平Rb1基因编辑效率。结果 RNP转染后的食蟹猴成纤维细胞提取基因组检测,发现其中有75%的细胞发生基因突变,且基因编辑类型多样,以碱基缺失突变为主;在胚胎水平上获取4-细胞期胚胎,检测单个卵裂球基因组序列发现,存在突变的胚胎占所有胚胎的36.4%,其中纯合突变比例为18.2%,嵌合突变比例同为18.2%。利用软件预测脱靶位点12个,进行序列测定,结果未发现脱靶。结论在非人灵长类细胞及胚胎水平上,CRISPR/Cas9基因编辑系统对于食蟹猴Rb1基因编辑有效,并且敲除效率较高,进一步利用基因编辑方法建立视网膜母细胞瘤模型研究中,可以作为有效的基因编辑手段。本研究得到的食蟹猴胚胎Rb1基因的编辑效率及胚胎囊胚率为建立视网膜母细胞瘤模型提供了参考。

关键词：视网膜母细胞瘤聚簇规则间隔短回文重复序列/相关核酸酶9 基因敲除打靶效率脱靶效率

Advancing application of mesenchymal stem cell-based bone tissue regeneration

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Bioactive Materials 2021年第3期6卷 666-683页

作者： Fengqing Shang Yang Yu Shiyu Liu Leiguo Ming Yongjie Zhang zhifei Zhou Jiayu zhao Yan Jin State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Key Laboratory of Oral DiseasesCenter for Tissue EngineeringFourth Military Medical UniversityXi'anShaanxi710032China Department of Stomatology The 306th Hospital of PLABeijing100101China Department of Periodontology School and Hospital of StomatologyCheeloo College of MedicineShandong University&Shandong Key Laboratory of Oral Tissue Regeneration&Shandong Engineering Laboratory for Dental Materials and Oral Tissue RegenerationJinanShandong250012China Department of Stomatology General Hospital of Tibetan Military CommandLhasa850000China Bureau of Service for Veteran Cadres of PLA in Beijing Beijing100001China

Reconstruction of bone defects,especially the critical-sized defects,with mechanical integrity to the skeleton is important for a patient's rehabilitation,however,it still remains *** biomaterials of human origin bone tissue for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural bone tissue with regard to its ***,not only efficacious and safe but also costeffective and convenient are important for regenerative biomaterials to achieve clinical translation and commercial *** in our understanding of regenerative biomaterials and their roles in new bone formation potentially opened a new frontier in the fast-growing field of regenerative *** inspiration from the role and multicomponent construction of native extracellular matrix(ECM)for cell accommodation,the ECM-mimicking biomaterials and the naturally decellularized ECM scaffolds were used to create new tissues for bone *** the other hand,with the going deep in understanding of mesenchymal stem cells(MSCs),they have shown great promise to jumpstart and facilitate bone healing even in diseased microenvironments with pharmacology-based endogenous MSCs rescue/mobilization,systemic/local infusion of MSCs for cytotherapy,biomaterials-based approaches,cell-sheets/-aggregates technology and usage of subcellular vesicles of MSCs to achieve scaffolds-free or cell-free delivery system,all of them have been shown can improve MSCsmediated regeneration in preclinical studies and several clinical ***,following an overview discussed autogenous/allogenic and ECM-based bone biomaterials for reconstructive surgery and applications of MSCsmediated bone healing and tissue engineering to further offer principles and effective strategies to optimize MSCs-based bone regeneration.

关键词： Mesenchymal stem cells Bone healing Regenerative medicine Biomaterials Extracellular matrix Cytotherapy Cell-sheets/-aggregates Exosomes

Mechanisms of resistance to trastuzumab in HER2-positive gastric cancer

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Chinese Journal of Cancer Research 2024年第3期36卷 306-321页

作者： zhifei Li Huan zhao Huihui Hu Haili Shang Yongjing Ren Wenhui Qiu Hao Su Huifang Lyu Xiaobing Chen Department of Oncology the Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer HospitalHenan Engineering Research Center of Precision Therapy of Gastrointestinal CancerZhengzhou Key Laboratory for Precision Therapy of Gastrointestinal CancerZhengzhou 450008China Henan University of Traditional Chinese Medicine Zhengzhou 450046China State Key Laboratory of Esophageal Cancer Prevention&Treatment Key Laboratory of Advanced Drug Preparation TechnologiesMinistry of EducationSchool of Pharmaceutical SciencesZhengzhou UniversityZhengzhou 450001China The First Affiliated Hospital of Xinxiang Medical University Xinxiang 453000China Department of Oncology the Affiliated Cancer Hospital of Zhengzhou University&Henan Cancer HospitalZhengzhou 450008China

Gastric cancer is one of the most prevalent cancers worldwide,and human epidermal growth factor receptor 2(HER2)-positive cases account for approximately 20%of the total ***,trastuzumab+chemotherapy is the recommended first-line treatment for patients with HER2-positive advanced gastric cancer,and the combination has exhibited definite efficacy in HER2-targeted ***,the emergence of drug resistance during treatment considerably reduces its effectiveness;thus,it is imperative to investigate the potential mechanisms underlying *** the present review article,we comprehensively introduce multiple mechanisms underlying resistance to trastuzumab in HER2-positive gastric cancer cases,aiming to provide insights for rectifying issues associated with resistance to trastuzumab and devising subsequent treatment strategies.

关键词： HER2 gastric cancer trastuzumab drug resistance

Pollen-inferred vegetation and environmental changes since 16.7 ka BP at Balikun Lake, Xinjiang

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Chinese Science Bulletin 2010年第22期55卷 2449-2457页

作者： TAO ShiChen AN ChenBang CHEN FaHu TANG LingYu WANG ZongLi LU YanBin LI zhifei ZHENG TongMing zhao JiaJu MOR Key Laboratory of West China's Environmental System Lanzhou University Lanzhou 730000 China Nanjing Institute of Geology and Paleontology Chinese Academy of Sciences Nanjing 210008 China

A high-resolution fossil pollen record from the sedimentary cores of Balikun Lake, northwestern China, combined with modern surface pollen data, is used to reconstruct the history of vegetation and climatic change since 16.7 cal. ka BP. Fossil pollen assem-blages and lithology indicate that the study area was dominated by desert. The desert had extremely arid climate and lower effec-tive moisture during 16.7–7.9 cal. ka BP, especially from 16.7 to 8.9 cal. ka BP when the lake maybe dried up. During 8.9–7.9 cal. ka BP, the environment gradually recovered in this area. It was then followed by the optimum period from 7.9 to 4.3 cal. Ka BP, when the effective moisture obviously increased. It was characterized by the typical desert-steppe/steppe vegetation and was ac-companied with several patch-birch woodlands around the lake. After that, a short but extremely arid climatic event occurred during 4.3–3.8 cal. ka BP, and the vegetation quickly changed from desert-steppe/steppe to desert. It was a relatively optimum period from 3.8 to 0.53 cal. ka BP showing typical desert-steppe/meadow-steppe landscape. Since 0.53 cal. ka BP, the climate has shown signs of deteriorating again. Furthermore, regional comparison shows that the characteristics of climatic and environmental evolution in this area were clearly different from East Asia monsoonal area during the last 16.7 cal. ka BP. It was characterized by the arid climate during the late-glacial and early Holocene, and relatively wet during the mid-late Holocene.

关键词：环境变化巴里坤湖花粉记录 BP 植被中晚全新世新疆气候变化

贝伐珠单抗联合同步放化疗在局部晚期头颈部鳞癌中的疗效观察

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解放军医学院学报 2016年第9期37卷 919-922,927页

作者：叶蕊赵志飞高灵灵吴璇牛保龙张欣悦邱栾李建雄解放军总医院放疗科北京100853 北京军区北戴河疗养院肿瘤科河北秦皇岛066100

目的评价贝伐珠单抗联合同步放化疗在局部晚期头颈部鳞癌中的近期疗效及安全性。方法选取解放军总医院2014年10月-2015年10月初治局部晚期头颈部鳞癌患者40例,随机分为两组。实验组（贝伐珠单抗组）：先给予贝伐珠单抗＋2周期多西他赛... 详细信息

目的评价贝伐珠单抗联合同步放化疗在局部晚期头颈部鳞癌中的近期疗效及安全性。方法选取解放军总医院2014年10月-2015年10月初治局部晚期头颈部鳞癌患者40例,随机分为两组。实验组（贝伐珠单抗组）：先给予贝伐珠单抗＋2周期多西他赛联合顺铂诱导化疗,后行贝伐珠单抗＋螺旋断层调强放疗＋同步顺铂化疗;对照组：先给予2周期多西他赛联合顺铂诱导化疗,后行螺旋断层调强放疗＋同步顺铂化疗。结果实验组和对照组在治疗结束后3个月客观缓解率分别为100%（20/20）和80%（16/20）,差异有统计学意义（P=0.000 2）。除淋巴细胞减少外,大部分不良反应为1~2级,两组不良反应发生率差异无统计学意义（P〉0.05）。结论贝伐珠单抗联合同步放化疗治疗头颈部鳞癌安全有效。

关键词：头颈部鳞癌贝伐珠单抗疗效

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4H型碳化硅高温氧化工艺研究

智能电网 2016年第6期4卷 546-549页

作者：钮应喜黄润华杨霏汪玲陈刚陶永洪刘奥柏松李赟赵志飞全球能源互联网研究院北京市昌平区102209 南京电子器件研究所江苏省南京市210016

在4H型碳化硅导电衬底上进行N型外延层生长,并对外延层表面进行化学机械抛光;通过高温氧化的方法在表面生成氧化层,并且在NO中进行退火;在此之后,加工碳化硅MOS电容和LMOS;通过C-V测试的方法对Si O2/Si C界面特性进行评估;通过测试的方... 详细信息

在4H型碳化硅导电衬底上进行N型外延层生长,并对外延层表面进行化学机械抛光;通过高温氧化的方法在表面生成氧化层,并且在NO中进行退火;在此之后,加工碳化硅MOS电容和LMOS;通过C-V测试的方法对Si O2/Si C界面特性进行评估;通过测试的方法对LMOS的场效应迁移率和MOS的击穿电压分布情况进行分析。结果表明,经过表面抛光可大大改善表面形貌,在一定程度上抑制界面态的产生,并且改善沟道导电的一致性。

关键词： 4H型碳化硅界面态碳化硅MOS电容可靠性

Going below and beyond the surface: Microneedle structure, materials, drugs, fabrication, and applications for wound healing and tissue regeneration

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Bioactive Materials 2023年第9期27卷 303-326页

作者： Shang Lyu zhifei Dong Xiaoxiao Xu Ho-Pan Bei Ho-Yin Yuen Chung-Wai James Cheung Man-Sang Wong Yong He Xin zhao Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHung HomHong Kong SARPR China Faculty of Science University of WaterlooWaterlooOntarioCanada State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhouPR China

Microneedle, as a novel drug delivery system, has attracted widespread attention due to its non-invasiveness, painless and simple administration, controllable drug delivery, and diverse cargo loading capacity. Although microneedles are initially designed to penetrate stratum corneum of skin for transdermal drug delivery, they, recently, have been used to promote wound healing and regeneration of diverse tissues and organs and the results are promising. Despite there are reviews about microneedles, few of them focus on wound healing and tissue regeneration. Here, we review the recent advances of microneedles in this field. We first give an overview of microneedle system in terms of its potential cargos (e.g., small molecules, macromolecules, nucleic acids, nanoparticles, extracellular vesicle, cells), structural designs (e.g., multidrug structures, adhesive structures), material selection, and drug release mechanisms. Then we briefly summarize different microneedle fabrication methods, including their advantages and limitations. We finally summarize the recent progress of microneedle-assisted wound healing and tissue regeneration (e.g., skin, cardiac, bone, tendon, ocular, vascular, oral, hair, spinal cord, and uterine tissues). We expect that our article would serve as a guideline for readers to design their microneedle systems according to different applications, including material selection, drug selection, and structure design, for achieving better healing and regeneration efficacy.

关键词： Microneedles Microfabrication Wound healing Tissue regeneration Drug delivery

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碳化硅MOSFET栅氧化层可靠性研究

智能电网 2015年第2期3卷 99-102页

作者：黄润华钮应喜杨霏陶永洪柏松陈刚汪玲刘奥卫能李赟赵志飞单片集成电路和模块国家级重点实验室江苏省南京市210016 国网智能电网研究院北京市昌平区102209 南京电子器件研究所江苏省南京市210016

通过TCAD仿真的方法对器件可靠性与结构设计之间的关系进行分析;对栅极电压和栅氧化层最强电场进行仿真,以对碳化硅金属氧化物半导体场效应晶体管(metal-oxide-semiconductor field-effect transistor,MOSFET)单胞结构参数进行优化;在N... 详细信息

通过TCAD仿真的方法对器件可靠性与结构设计之间的关系进行分析;对栅极电压和栅氧化层最强电场进行仿真,以对碳化硅金属氧化物半导体场效应晶体管(metal-oxide-semiconductor field-effect transistor,MOSFET)单胞结构参数进行优化;在N型碳化硅外延层上制作金属氧化物半导体(metal-oxide-semiconductor,MOS)电容,并且通过对MOS电容进行C-V测试的方法评估Si O2/Si C界面质量。对导带附近界面陷阱密度进行比较。NO退火的样品与干氧氧化样品相比界面质量明显改善,界面态密度小于5×10~11 cm–2e V–1。

关键词：界面态碳化硅金属氧化物半导体(metal-oxide-semiconductor,MOS)电容可