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Fluorescent and colorimetric immunoassay of nuclear matrix protein 22 enhanced by porous Pd nanoparticles

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Chinese Chemical Letters 2019年第6期30卷 1307-1309页

作者： Wenyun Zhuge Xiaofeng Tan ruyue zhang He Li Gengxiu Zheng School of Chemistry and Chemical Engineering University of Jinan

A modified ELISA realizing fluorescent and colorimetric immunoassay of nuclear matrix protein 22 (NMP 22) was developed based on porous Pd nanoparticles. The unique structure and excellent enzyme mimetic activity of porous Pd nanoparticles favor to oxidize o-phenylenediamine (OPD) into 2,3-phenazinediamine (oxOPD) by H2O2, producing colorimetric and fluorescence dual-readout signal for the detection of NMP 22. The developed immunoassay method will offer great potential in clinical research and diagnostic applications.

关键词： Nuclear matrix protein 22 Porous Pd nanoparticles ELISA Colorimetric Fluorescence

Emerging Evidence Linking the Liver to the Cardiovascular System: Liver-derived Secretory Factors

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Journal of Clinical and Translational Hepatology 2023年第5期11卷 1246-1255页

作者： Xiang Liu Yijia Shao Linjiang Han ruyue zhang Jimei Chen Department of Cardiac Surgery Guangdong Cardiovascular InstituteGuangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhouGuangdongChina Guangdong Provincial Key Laboratory of South China Structural Heart Disease GuangzhouGuangdongChina Department of Geriatrics The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdongChina

Cardiovascular diseases(CVDs)remain the leading cause of morbidity and mortality ***,accumulating evidence has revealed hepatic mediators,termed as liverderived secretory factors(LDSFs),play an important role in regulating CVDs such as atherosclerosis,coronary artery disease,thrombosis,myocardial infarction,heart failure,metabolic cardiomyopathy,arterial hypertension,and pulmonary *** presented here consisted of microbial metabolite,extracellular vesicles,proteins,and microRNA,they are primarily or exclusively synthesized and released by the liver,and have been shown to exert pleiotropic actions on cardiovascular *** mainly target vascular endothelial cell,vascular smooth muscle cells,cardiomyocytes,fibroblasts,macrophages and platelets,and further modulate endothelial nitric oxide synthase/nitric oxide,endothelial function,energy metabolism,inflammation,oxidative stress,and dystrophic *** some LDSFs are known to be detrimental/beneficial,controversial findings were also reported for ***,more studies are required to further explore the causal relationships between LDSFs and CVDs and uncover the exact mechanisms,which is expected to extend our understanding of the crosstalk between the liver and cardiovascular system and identify potential therapeutic ***,in the case of patients with liver disease,awareness should be given to the implications of these abnormalities in the cardiovascular *** studies also underline the importance of early recognition and intervention of liver abnormalities in the practice of cardiovascular care,and a multidisciplinary approach combining hepatologists and cardiologists would be more preferable for such patients.

关键词： Liver Cardiovascular system Metabolite Extracellular vesicle Hepatokine

The chromatin remodeler BRAHMA recruits HISTONE DEACETYLASE6 to regulate root growth inhibition in response to phosphate starvation in Arabidopsis

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Journal of Integrative Plant Biology 2022年第12期64卷 2314-2326页

作者： Tao Li ruyue zhang Viswanathan Satheesh Peng Wang Guojie Ma Jianfei Guo Guo-Yong An Mingguang Lei Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China

Plasticity in root system architecture(RSA)allows plants to adapt to changing nutritional status in the *** availability is a major determinant of crop yield,and RSA remodeling is critical to increasing the efficiency of phosphorus *** substantial progress has been made in understanding the signaling mechanism driving phosphate starvation responses in plants,whether and how epigenetic regulatory mechanisms contribute is poorly ***,we report that the Switch defective/sucrose non-fermentable(SWI/SNF)ATPase BRAHMA(BRM)is involved in the local response to phosphate(Pi)*** loss of BRM function induces iron(Fe)accumulation through increased LOW PHOSPHATE ROOT1(LPR1)and LPR2 expression,reducing primary root length under Pi *** also demonstrate that BRM recruits the histone deacetylase(HDA)complex HDA6-HDC1 to facilitate histone H3 deacetylation at LPR loci,thereby negatively regulating local Pi deficiency *** is degraded under Pi deficiency conditions through the 26 S proteasome pathway,leading to increased histone H3 acetylation at the LPR ***,our data suggest that the chromatin remodeler BRM,in concert with HDA6,negatively regulates Fe-dependent local Pi starvation responses by transcriptionally repressing the RSA-related genes LPR1 and LPR2 in Arabidopsis thaliana.

关键词： BRM histone deacetylation phosphate starvation root development

Biocatalytic stereoselective synthesis of methyl mandelates by engineering a cytochrome P450 hydroxylase

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Green Synthesis and Catalysis 2024年第2期5卷 88-93页

作者： Lingzhi Xie Yun zhang ruyue zhang Haibo Cui Baodong Cui Wenyong Han Nanwei Wan Zhi Li Yongzheng Chen Key Laboratory of Biocatalysis&Chiral Drug Synthesis of Guizhou Province Generic Drug Research Center of Guizhou ProvinceGreen Pharmaceuticals Engineering Research Center of Guizhou ProvinceSchool of PharmacyZunyi Medical UniversityZunyi 563000China Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical UniversityZunyi 563000China Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4Singapore 117585Singapore

Chiral methyl mandelates are useful synthons in organic transformation and pharmaceutical *** synthesis of these valuable compounds by direct C–H activating oxidative hydroxylation has attracted keen *** herein is achieving the stereoselective and efficient bio-hydroxylation of methyl 2-phenylacetates to the chiral methyl mandelates by directed evolution of the cytochrome P450DA *** the present study,a new colorimetric high-throughput screening assay was successfully developed based on a dualenzyme cascade for the engineering of the P450DA's hydroxylation *** beneficial variants with enhanced bio-hydroxylation activity were created by combining random mutagenesis and site-saturated/directed mutagenesis ***-cell bio-hydroxylation of various methyl 2-phenylacetates using the best septupletmutant P450DA-11 yielded the corresponding chiral methyl mandelates in up to 92%isolated yields and>99%ee.

关键词： Bio-hydroxylation Directed evolution Cytochrome P450 High-throughput screening Methyl mandelates

Inositol Pyrophosphate lnsP8 Acts as an Intracellular Phosphate Signal in Arabidopsis

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Molecular Plant 2019年第11期12卷 1463-1473页

作者： Jinsong Dong Guojie Ma Liqian Sui Mengwei Wei Viswanathan Satheesh ruyue zhang Shenghong Ge Jinkai Li Tong-En zhang Christopher Wittwer Henning JJessen Huiming zhang Guo-Yong An Dai-Yin Chao Dong Liu Mingguang Lei Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China National Key Laboratory of Plant Molecular Genetics CAS Centre for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology&EcologyChinese Academic of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China lnstitute of Organic Chemistry Albert-Ludwigs UniversityFreiburgAlbertstrasse 2179104 FreiburgGermany C旧SS-Centre for Integrative Biological Signalling Studies Albert-Ludwigs UniversityFreiburgAlbertstrasse 2179104 FreiburgGermany Key Laboratory of Plant Stress Biology School of Life SciencesHenan University.Kaifeng 475004China Center for Plant Biology School of Life SciencesTsinghua UniversityBeijing 100084China

The maintenance of cellular phosphate(Pi)homeostasis is of great importance in living *** SPX domain-containing protein 1(SPX1)proteins from both Arabidopsis and rice have been proposed to act as sensors of Pi *** molecular signal indicating the cellular Pi status and regulating Pi homeostasis in plants,however,remains to be identified,as Pi itself does not bind to the SPX ***,we report the identification of the inositol pyrophosphate lnsP8 as a signaling molecule that regulates Pi homeostasis in *** gel electrophoresis profiling of InsPs revealed that lnsP8 level positively correlates with cellular Pi *** demonstrated that the homologs of diphosphoinositol pentaki-sphosphate kinase(PPIP5K),VIH1 and VIH2,function redundantly to synthesize lnsP8,and that the vih1 vih2 double mutant overaccumulates ***1 directly interacts with PHR1,the central regulator of Pi starvation responses,to inhibit its function under Pi-replete ***,this interaction is compromised in the vih1 vih2 double mutant,resulting in the constitutive induction of Pi starvation-induced genes,indicating that plant cells cannot sense cellular Pi status without ***,we showed that lnsP8 could directly bind to the SPX domain of SPX1 and is essential for the interaction between SPX1 and ***,our study suggests that lnsP8 is the intracellular Pi signaling molecule serving as the ligand of SPX1 for controlling Pi homeostasis in plants.

关键词： nositol pyrophosphates lnsP8 phosphate signal SPX domain Pi homeostasis

Mediating Role of Glucose-Lipid Metabolism in the Association between the Increased Risk of Coronary Heart Disease and Exposure to Organophosphate Esters,Phthalates,and Polycyclic Aromatic Hydrocarbons

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Environment & Health 2024年第3期2卷 170-179页

作者： Duo zhang Xiang Liu Jiazichao Tu Qinru Xiao Linjiang Han Jinfeng Fu Junye Bian ruyue zhang Jimei Chen Yijia Shao Shaoyou Lu School of Public Health(Shenzhen) Sun Yatsen UniversityShenzhen 518107China Department of Cardiac Surgery Guangdong Cardiovascular InstituteGuangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China

There is a lack of human evidence concerning the cardiovascular effects of combined exposure to endocrine *** case-control study sought to investigate coronary heart disease(CHD)associations with exposure to organophosphate flame retardants(OFRs),phthalates(PAEs),and polycyclic aromatic hydrocarbons(PAHs)among 148 adults with coronary-angiography-diagnosed CHD and 320 healthy adults from southern *** mediating role of glucose-lipid metabolism was also *** kernel machine regression suggested that when exposure status was fixed to the 75th percentile with the median value as the reference,exposure to OFRs,PAEs,and PAHs was associated with an 84%(95%CI:36%−134%),132%(12%−252%),and 214%(89%−331%)increased risk of developing CHD,*** quantile sum regression indicated urinary bis(2-butoxyethyl)phosphate(BBOEP),dibutyl phosphate(DBP),monoisononyl phthalate(miNP),and metabolites of phenanthrene may be major contributors to the overall effect of *** further analyses on identified chemical risk factors,mediation analyses suggested exposure to phenanthrene may increase the risk of CHD via elevating total cholesterol and blood glucose,while exposure to DiNP mainly associates with serum ***,we observed a slight mediation effect of oxidative DNA damage between urinary BBOEP and risk of *** results provide potential direction for further experimental *** evidence is needed to clarify the causation of the results.

关键词： Cardiovascular disease Flame retardants Plasticizers Combined exposure analysis Oxidative stress

New speckle pattern interferometry for precise in situ deformation measurements

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Chinese Optics Letters 2024年第1期22卷 34-40页

作者：章如月 Yu Fu 缪泓 CAS Key Laboratory of Mechanical Behavior and Design of Materials Department of Modern MechanicsUniversity of Science and Technology of ChinaHefei 230027China Shenzhen Key Laboratory of Intelligent Optical Measurement and Detection College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China

A new electronic speckle pattern interferometry method is proposed to realize in situ deformation *** feature of the method is the combination of a high-speed camera and multiple laser Doppler vibrometers(LDVs)for synchronous *** high-speed camera is used to record and select effective interferograms,while the LDVs are used to measure the rigid body displacement caused by vibrations.A series of effective interferograms with known shifted phase values are obtained to calculate the deformation *** experimental results show that the method performs well in measuring static and dynamic deformations with high accuracy in vibrating environments.

关键词： speckle pattern interferometry laser Doppler vibrometers in situ deformation measurements

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温度应力和支座约束对钢结构网架的影响分析

建筑结构 2022年第S2期52卷 583-586页

作者：张如月安然保定市建筑设计院有限公司保定071000

大跨度钢结构网架的杆件容易受温度的影响而伸长或缩短从而产生温度应力,在多次温度应力交替作用下钢结构连接处的焊缝成为应力集中点,有可能产生破坏使结构失效[1];网架结构的支座节点应能保证平安牢靠的传递支承反力,由于钢网架结构... 详细信息

大跨度钢结构网架的杆件容易受温度的影响而伸长或缩短从而产生温度应力,在多次温度应力交替作用下钢结构连接处的焊缝成为应力集中点,有可能产生破坏使结构失效[1];网架结构的支座节点应能保证平安牢靠的传递支承反力,由于钢网架结构是高次超静定的杆件体系,不同的约束条件有可能导致杆件内力和支座反力的转变,这将直接影响杆件的内力和位移,且若支座节点的构造不符合计算假定,甚至还会造成杆件内力变号的情况。所以杆件的温度应力和支座形式应受到足够的重视。通过调整不同的温度应力和支座约束形式,对比分析了不同工况下钢结构网架的计算结果,分析了温度应力和支座约束对钢结构网架各项指标的影响,旨在最大程度上提高钢网架结构的安全性、经济性及合理性。

关键词：钢结构网架温度应力支座约束

农村教师专业可持续发展策略及启示--以Y市分黄学区为例

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成才之路 2022年第28期 37-40页

作者：张茹月三峡大学田家炳教育学院湖北宜昌443000

乡村振兴战略规划指出,要优先发展农村教育事业。农村学校是我国学校教育的重点发展对象,农村学校教师则是农村教育发展的中流砥柱。Y市分黄学区坚持“教师共同体”的治校理念,即学区教师在学科教学与学校管理中共同参与、通力合作,实... 详细信息

乡村振兴战略规划指出,要优先发展农村教育事业。农村学校是我国学校教育的重点发展对象,农村学校教师则是农村教育发展的中流砥柱。Y市分黄学区坚持“教师共同体”的治校理念,即学区教师在学科教学与学校管理中共同参与、通力合作,实现了农村学校教师的专业可持续发展,该学区的教师发展策略也为其他地区的农村中小学教师专业发展提供了宝贵的经验。文章以Y市分黄学区为例探讨农村教师专业可持续发展策略。

关键词：农村学校中小学教师专业能力可持续发展