Inositol Pyrophosphate lnsP8 Acts as an Intracellular Phosphate Signal in Arabidopsis

作     者：Jinsong Dong Guojie Ma Liqian Sui Mengwei Wei Viswanathan Satheesh Ruyue Zhang Shenghong Ge Jinkai Li Tong-En Zhang Christopher Wittwer Henning JJessen Huiming Zhang Guo-Yong An Dai-Yin Chao Dong Liu Mingguang Lei 

作者机构：Shanghai Center for Plant Stress BiologyCAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China National Key Laboratory of Plant Molecular GeneticsCAS Centre for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology&EcologyChinese Academic of SciencesShanghai 200032China University of Chinese Academy of SciencesBeijing 100049China lnstitute of Organic ChemistryAlbert-Ludwigs UniversityFreiburgAlbertstrasse 2179104 FreiburgGermany C旧SS-Centre for Integrative Biological Signalling StudiesAlbert-Ludwigs UniversityFreiburgAlbertstrasse 2179104 FreiburgGermany Key Laboratory of Plant Stress BiologySchool of Life SciencesHenan University.Kaifeng 475004China Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2019年第12卷第11期

页      面：1463-1473页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the Chinese Academy of Sciences(CAS)and the Science and Technology Commission of Shanghai Municipality supported by the German Research Foundation(DFG)under Germany’s Excellence Strategy(CIBSS-EXC-2189-Project ID) 

主　　题：nositol pyrophosphates lnsP8 phosphate signal SPX domain Pi homeostasis 

摘      要：The maintenance of cellular phosphate(Pi)homeostasis is of great importance in living *** SPX domain-containing protein 1(SPX1)proteins from both Arabidopsis and rice have been proposed to act as sensors of Pi *** molecular signal indicating the cellular Pi status and regulating Pi homeostasis in plants,however,remains to be identified,as Pi itself does not bind to the SPX ***,we report the identification of the inositol pyrophosphate lnsP8 as a signaling molecule that regulates Pi homeostasis in *** gel electrophoresis profiling of InsPs revealed that lnsP8 level positively cor­relates with cellular Pi *** demonstrated that the homologs of diphosphoinositol pentaki-sphosphate kinase(PPIP5K),VIH1 and VIH2,function redundantly to synthesize lnsP8,and that the vih1 vih2 double mutant overaccumulates ***1 directly interacts with PHR1,the central regulator of Pi star­vation responses,to inhibit its function under Pi-replete ***,this interaction is compro­mised in the vih1 vih2 double mutant,resulting in the constitutive induction of Pi starvation-induced genes,indicating that plant cells cannot sense cellular Pi status without ***,we showed that lnsP8 could directly bind to the SPX domain of SPX1 and is essential for the interaction between SPX1 and ***,our study suggests that lnsP8 is the intracellular Pi signaling molecule serving as the ligand of SPX1 for controlling Pi homeostasis in plants.