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Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay

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Chinese Journal of Electronics 2017年第1期26卷 152-155页

作者： LIU Juntao QU Shuxue LUO Jinping WANG Bin cai xinxia State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences University of Chinese Academy of Sciences

Carcinoembryonic antigen(CEA) is one of the most widely used tumor markers worldwide. A portable system was designed for rapid field specific test of CEA based on immunomagnetic separation and chemiluminescence immunoassay, which consisted of chemiluminescent immunosensor and optical test instrument. The chemiluminescent immunosensor was based on immunomagnetic separation and chemiluminescence immunoassay, which utilized a immuno-sandwich scheme as-say with Horseradish peroxidase(HRP) labeled anti-CEA antibody and Immunomagnetic beads(IMBs). And the test instrument consisted of optical detection module and central processing and control system. The optical noise of this instrument was below 40 photons/s. The system could achieve a sample test in 30 minutes, and the detecting result showed a good liner relationship between the intensity of luminescence and concentration of CEA in the range from 1 to1000ng/m L, with a correlation coefficient of 0.9858. Real human samples were also detected with this system in comparison with clinical results and a correlation coefficient of0.9934 was obtained. The instrument is portable and easy to operate, which will provide potential application in cancer clinical test.

关键词： Chemiluminescence immunoassay Carcinoembryonic antigen Rapid field test Optical sensor FPGA NIOS II processor

A NEW QUANTITATIVE DETECTION METHOD OF RECOMBINANT CFP10-ESAT6 AMALGAMATION PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS BASED ON MICRO-MAGNETIC PROBES STRATEGY

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Journal of Innovative Optical Health Sciences 2012年第1期5卷 55-60页

作者： YIQING HUANG JINPING LUO MIXIA WANG JUNTAO LIU xinxia cai State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100190China Graduate School of Chinese Academy of Sciences Beijing 100190 China

A new rapid,specific and sensitive method for assay of recombinant CFP10-ESAT6 amalgamation proteins from Mycobacterium tuberculosis was *** method used streptavidincoated magnetic beads to enrich the specific biotinylated anti-CFP10 antibody,then adopted a sandwich-type enzyme linked immunosorbent assay technology with two kinds of monoclonal antibodies:biotinylated anti-CFP10 antibody and HRP-labeled anti-CFP10 antibody to identify the target CFP10-ESAT6 proteins,and finally detected chemiluminescence intensity by a small home-made optical *** was shown that,the corresponding chemiluminescence intensity had a good logarithmic linear response to the concentration of CFP10-ESAT6 proteins when ranging at 1~1000 ng/mL,and the correlation coefficient is *** proposed method could detect the CFP10-ESAT6 proteins with low detection limit(1 ng/mL)and the detection time could be controlled within 45 *** with commonly used detection methods of ***,this method was easy to operate,faster,and of higher *** achievement of the quantitative detection of CFP10-ESAT6 proteins has important scientific significance and wide application prospects in tuberculosis control.

关键词： Enzyme linked immunosorbent assay chemiluminescence home-made optical sensor

Dopamine detection using a patch-clamp system on a planar microeletrode array electrodeposited by polypyrrole/graphene nanocomposites

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Science China(Technological Sciences) 2014年第2期57卷 288-292页

作者： WANG Li JIANG TingJun SONG YiLin SHI WenTao cai xinxia State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences University of Chinese Academy of Sciences

To achieve a dopamine （DA） response with high sensitivity and high signal-to-noise ratio （S/N） with a patch-clamp system, polypyrrole/graphene （PPy/GR） nanocomposites were steadily electrodeposited by an electrochemical method on a planar mi- croelectrode array （pMEA） fabricated by a standard micromachining process. The electrodeposition process was carried out by chronopotentimetry measurement scanning from 0.1 to 0.8 C/cm2 at the current of 2 mA; 0.5 C/cm2 was found to be optimal. The pMEA modified by PPy/GR at the 0.5 C/cm2 exhibits remarkable properties; for instance, the standard deviation （SD） de- creases from 8.4614×10-al to 5.62×10 11 A, reduced by 33.52%, and the sensitivity increases from 2566.88 to 76114.65 gAmMcm2 , 29.65 times higher than the bare Pt （platinum）. A good linear relationship between the current and DA concentra- tion in the range of 0.30 to 61.71 grn was obtained, with a correlation coefficient of 0.997. The sensor is meaningful for neuro- science research and the treatment of neurological diseases.

关键词： polypyrrole/graphene planar microelectrode array electrodeposition dopamine

Disposable Electrochemical Biosensor for Amperometric Measurement of β-hydroxybutyrate and Total Cholesterol

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稀有金属材料与工程 2006年第A3期35卷 305-308页

作者： Huaqing Li Li Wang Nenggao Rao Dafu Cui xinxia cai State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100080 China Graduate School of Chinese Academy of Science Beijing 100080 China

A new type of disposable thin-film amperometric biosensor has been developed for measurement ofβ-hydroxybutyrate and total cholesterol in *** biosensor consists of two plain gold electrodes mounted on a Polyethylene terephthalate (PET) *** reagent solution contains bienzymes (cholesterol oxidase and cholesterol esterase for total cholesterol,β-hydroxybutyrate dehydrogenase and diaphorase forβ-hydroxybutyrate respectively) with mediator (ferricyanide) were absorption at the surface of electrodes coated by electrodepositing platinum *** presence of the mediator lowers the applied potential and eliminates the interference from other oxidizable species enhancing the sensitivity and selectivity of the biosensor without modifying the dynamic parameters of the *** enzymes stably retains in the matrices of platinum black film improving the performance of the mediated *** linearity is observed in the concentration range from 1.0×10^(-4) to 1.0×10^(-2) mM and 1.0×10^(-4) mM to 4.99×10^(-3) mM with sensitivity of 1.958μA/mM and 2.447μA/mM *** optimized biosensor exhibits excellent reproducibility and stability retaining more than 90% of its original activity over a period of one *** simple operation of the biosensor mass-produced at low cost is expected to find clinical application and homecare.

关键词： amperometric β-Hydroxybutyrate total cholesterol ferricyanide platinum black

Development of Enzyme Biosensor for Amperometric Measurement of Aspartate Aminotransferase

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稀有金属材料与工程 2006年第A3期35卷 330-332页

作者： Zonghui Guo Chunxiu Liu Huaqing Li Jianfang Xu Lianshi Feng xinxia cai State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100080 China Graduate School of Chinese Academy of Science Beijing 100080 China Biology Center China Institute of Sport Science Beijing 100080 China

An enzyme biosensor for amperometric measurement of aspartate aminotransferase has been *** working electrode was modified with a thin-film of redox polymer,then glutamate oxidase,with the immobilized reagent cast and dried on the *** biosensor responses to AST by detecting hydrogen peroxide were produced by enzymical reaction at-0.1 V with a response time of 120 *** electrode gave a detection limit of 32.5 U/L with a linear concentration range of 32.5 U/L～2000 U/L in *** to more sensitive and lower detection limit,the biosensor is expected mainly to be used for physiological identification and physical performance of athletes in the *** application will also affect the practice of clinical medicine for the diagnosis of heart and liver disease.

关键词： aspartate aminotransferase redox polymer amperometric biosensor

Amperometric Enzyme Biosensor for the Determination of High Concentration Lactate in Whole Blood of Athletes

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稀有金属材料与工程 2006年第A3期35卷 83-86页

作者： Chunxiu Liu Zonghui Guo Hui Wang Jianfang Xu Lianshi Feng xinxia cai State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100080 China Graduate School of Chinese Academy of Sciences Beijing 100080 China Biology Center China Institute of Sport Science Beijing 100061 China

Amperometric biosensor applied to the determination of high concentration lactate in serum and whole blood was *** biosensor was constructed by gold electrode modified with nanoplatinum *** oxidase (E.C.1.***.3.2) was immobilized at platinized activated gold electrode which was used for the determination of high concentration lactate at low potential (+0.2 V).The linear calibration graphs were obtained from 1 to 21 mmol·L~ (-1) lactate in serum and from 0.9 to 13.2 mmol.L~ (-1) lactate in whole *** correlation coefficients were 0.99 and 0.97,respectively at a steady-state response time of 50 s.

关键词： biosensors lactate oxidase high concentration lactate whole blood

A Point-of-Care Test System for Biochemical Blood Analysis Based on ADUC824

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稀有金属材料与工程 2006年第A3期35卷 75-79页

作者： Hui Wang Qing Tian Liying Jiang Chunxiu Liu Zonghui Guo xinxia cai State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Science Chinese Academy of Sciences Beijing 100080 China Graduate School of Chinese Academy of Sciences Beijing 100080 China

A point-of-care test system has been studied in this *** was used to determine substances in blood such as Hemoglobin (HB),Aspartate Aminotransferase (AST),Creatine Kinase (CK) and so *** on the principle of amperometric determination,the research on detecting weak current signals was carried *** the same time as to the weak signals (nA level),magnifying,sampling and processing the signals were also *** by ADUC824 and assisted by other units, every substance could be determined automatically and rapidly integrated with the corresponding *** the experiment, the minimum detectable current of the instrument (YT2005-1) is 0.2 *** regard to the 1 nA which the experiment demanded,it could be up to the *** the system can provide results in 180 s with a long term stability.

关键词： point-of-care test ADUC824 weak signals HB,AST and CK

Microelectrode arrays for monitoring neural activity in neural stem cells with modulation by glutamate in vitro

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Nanotechnology and Precision Engineering 2020年第2期3卷 69-74页

作者： Fei Gao Jinping Luo Yilin Song Enhui He Yu Zhang Guihua Xiao xinxia cai State Key Laboratory of Transducer Technology Institute of ElectronicsChinese Academy of SciencesBeijing 100190PR China University of Chinese Academy of Sciences Beijing 100049PR China Institute of Electronics Chinese Academy of Sciences

In this study, a 60-channel microelectrode array(MEA) was fabricated and used to monitor the neural spikes and local field potentials(LFPs) of neurons differentiated from rat neural stem cells in vitro. The neurons were grown on the MEA surface to detect neural signals. Glutamate(Glu) was used to modulate neural activity during experiments. To enhance detection performance, platinum nanoparticles were modified onto the microelectrode site surface. Glutamate stimulated neural spikes and LFPs were recorded using the MEA. The average spike amplitude was approximately 70 μV in the normal state. The spike amplitude increased by 29% from 70 μV to 90 μV with Glu modulation. The firing rate increased by 69% from 4.01 Hz to 6.8 Hz with Glu modulation. The LFP power increased from 326 μW in the normal state to 617 μW with Glu modulation in the 0–10 Hz frequency band. Data analysis shows that neural activity stimulated by Glu modulation was recorded experimentally at high temporal-spatial resolution. These results may provide a new neuron detection method, as well as further understanding of neural stem cell spike firing and associated mechanisms.

关键词： MEA Glutamate Spikes Neural stem cells In vitro

Small-volume solution current-time behavior study for application in reverse iontophoresis-based non-invasive blood glucose monitoring

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Science China Chemistry 2011年第1期54卷 223-230页

作者： CHANG LingQian LIU ChunXiu HE YinZeng XIAO HongHui cai xinxia State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100190 China Graduate University of Chinese Academy of Sciences Beijing 100190 China

The reverse iontophoresis-based glucose monitoring circumstance is similar to the small-volume solution in which mass diffusion controls the current response of the electrochemical *** this study,the law of mass transfer in this type of solution was analyzed and a mathematic model was established to depict the current-time behavior of the fabricated planar electrode used in the non-invasive meter designed by ourselves.A small-volume glucose solution was directly constructed on the electrode to simulate the reverse iontophoresis-based sensing *** correctness of the model was demonstrated by *** assay was subsequently carried out to verify the practicality of the model in determination of blood *** results processed by the new method accurately traced the authentic value,confirming the advantage of the new method and the potential in clinical analysis.

关键词： non-invasive detection current.time behavior small-volume solution mass transfer semi-infinite diffusion mathematic model