A NEW QUANTITATIVE DETECTION METHOD OF RECOMBINANT CFP10-ESAT6 AMALGAMATION PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS BASED ON MICRO-MAGNETIC PROBES STRATEGY
作者机构：State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of SciencesBeijing 100190China Graduate School of Chinese Academy of Sciences Beijing 100190China
出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)
年 卷 期：2012年第5卷第1期
页 面：55-60页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：This project is supported by National Major Scientific Research Program of China(No.2011CB933202) National High Technology Research and Development Program of China(No.2009AA03Z411) National Natural Science Foundation of China(No.61002037,61101048) Knowledge Innovation Program of The Chinese Academy of Sciences(CXJJ-10-M31,KGCX2-YW-916)
主 题：Enzyme linked immunosorbent assay chemiluminescence home-made optical sensor
摘 要：A new rapid,specific and sensitive method for assay of recombinant CFP10-ESAT6 amalgamation proteins from Mycobacterium tuberculosis was *** method used streptavidincoated magnetic beads to enrich the specific biotinylated anti-CFP10 antibody,then adopted a sandwich-type enzyme linked immunosorbent assay technology with two kinds of monoclonal antibodies:biotinylated anti-CFP10 antibody and HRP-labeled anti-CFP10 antibody to identify the target CFP10-ESAT6 proteins,and finally detected chemiluminescence intensity by a small home-made optical *** was shown that,the corresponding chemiluminescence intensity had a good logarithmic linear response to the concentration of CFP10-ESAT6 proteins when ranging at 1~1000 ng/mL,and the correlation coefficient is *** proposed method could detect the CFP10-ESAT6 proteins with low detection limit(1 ng/mL)and the detection time could be controlled within 45 *** with commonly used detection methods of ***,this method was easy to operate,faster,and of higher *** achievement of the quantitative detection of CFP10-ESAT6 proteins has important scientific significance and wide application prospects in tuberculosis control.
维普期刊数据库