Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay
Portable System for Rapid Field Test of CEA in Human Serum Based on Chemiluminescence Enzyme Immunoassay作者机构：State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences University of Chinese Academy of Sciences
出 版 物：《Chinese Journal of Electronics》 (电子学报(英文))
年 卷 期：2017年第26卷第1期
页 面：152-155页
核心收录：
学科分类：1002[医学-临床医学] 0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080202[工学-机械电子工程] 0802[工学-机械工程] 100214[医学-肿瘤学] 0701[理学-数学] 10[医学]
基 金：supported by the National Basic Research Program of China(No.2014CB744600) the National Natural Science Foundation of China(No.61527815,No.61471342) the Key Programs of the Chinese Academy of Sciences(No.KJZD-EW-L11-2) the Bejing Municipal science and Technology Commission Program(No.Z141100000214002)
主 题：Chemiluminescence immunoassay Carcinoembryonic antigen Rapid field test Optical sensor FPGA NIOS II processor
摘 要：Carcinoembryonic antigen(CEA) is one of the most widely used tumor markers worldwide. A portable system was designed for rapid field specific test of CEA based on immunomagnetic separation and chemiluminescence immunoassay, which consisted of chemiluminescent immunosensor and optical test instrument. The chemiluminescent immunosensor was based on immunomagnetic separation and chemiluminescence immunoassay, which utilized a immuno-sandwich scheme as-say with Horseradish peroxidase(HRP) labeled anti-CEA antibody and Immunomagnetic beads(IMBs). And the test instrument consisted of optical detection module and central processing and control system. The optical noise of this instrument was below 40 photons/s. The system could achieve a sample test in 30 minutes, and the detecting result showed a good liner relationship between the intensity of luminescence and concentration of CEA in the range from 1 to1000ng/m L, with a correlation coefficient of 0.9858. Real human samples were also detected with this system in comparison with clinical results and a correlation coefficient of0.9934 was obtained. The instrument is portable and easy to operate, which will provide potential application in cancer clinical test.
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