检索结果-南通市图书馆

Mediating Mesenchymal Stem Cells Responses and Osteopontin Adsorption via Oligo(ethylene glycol)-amino Mixed Self-assembled Monolayers

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2016年第9期32卷 966-970页

作者： Lijing Hao Tianjie Li naru zhao Fuzhai Cui Chang Du Yingjun Wang School of Materials Science and Engineering South China University of TechnologyGuangzhou 510640China National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou 510006China Department of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

Oligo（ethylene glycol）（-OEG） and amino （-NH2） mixed self-assembled monolayers （SAMs） were employed as model substrates to investigate the effect of charge density on the fate of mesenchymal stem cells （MSCs） and osteopontin （OPN） adsorption. We found that all surfaces presenting -NH2 groups favored cell responses regardless of the surface charge. Meanwhile, OPN adsorption could remain stable on the mixed SAMs over a certain range of charge densities. Our work provides some insights into cell responses and protein adsorption to surface charge.

关键词： Mixed self-assembled monolayers Oligo(ethylene glycol)-amino Mesenchymal stem cell Osteopontin

Three-dimensional printing of β-tricalcium phosphate/calcium silicate composite scaffolds for bone tissue engineering

在线全文

学校读者我要写书评

暂无评论

Bio-Design and Manufacturing 2018年第2期1卷 146-156页

作者： Yifan Dong Haibo Duan naru zhao Xiao Liu Yijuan Ma Xuetao Shi School of Materials Science and Engineering South China University of Technology Guangzhou 510641 China National Engineering Research Centre for Tissue Restoration and Reconstruction Guangzhou 510006 China Guangdong Province Key Laboratory of Biomedical Engineering South China University of Technology Guangzhou 510006 China South China Institute of Collaborative Innovation Dongguan China

Bioactive scaffolds with interconnected porous structures are essential for guiding cell growth and new bone formation. In this work, we successfully fabricated three-dimensional （3D） porous β-tricalcium phosphate （β-TCP）/calcium silicate （CS） composite scaffolds with different ratios by 3D printing technique and further investigated the physiochemical properties, in vitro apatite mineralization properties and degradability of porous β-TCP/CS scaffolds. Moreover, a series of in vitro cell experiments including the attachment, proliferation and osteogenic differentiation of mouse bone marrow stromal cells were conducted to testify their biological performances. The results showed that 3D printed β-TCP/CS scaffolds possessed of controllable internal porous structures and external shape. Furthermore, the introduction of CS decreased the shrinkage of scaffolds and improved the in vitro apatite formation activity and degradation rate. Meanwhile, compared with pure β- TCP scaffold, the β-TCP/CS composite scaffolds were more conducive to promote cell adhesion, spread and osteogenesis differentiation. However, when the content of CS was increased to 45%, the ions dissolution rate of the composite scaffolds was so high that leaded to the increase in pH value, which inhibited the proliferation of cells. Our results suggested that the introduction of appropriate CS into β-TCP bioceramic is an effective strategy to prepare bioactive 3D printed bioceramic scaffolds for hard tissue regeneration.

关键词： 3D printing β-Tricalcium phosphate/calcium silicate Osteogenesis differentiation Tissue regeneration

Characterization of bioactive ceramic coatings prepared on titanium implants by micro-arc oxidation

在线全文

学校读者我要写书评

暂无评论

Rare Metals 2006年第1期25卷 84-89页

作者： WANG Yingjun NAN Kaihui CHEN Xiaofeng NING Chengyun WANG lingyu zhao naru The Key Laboratory for Specially Functional Materials and Advanced Manufacturing Technology Ministry of Education South China University of Technology Guangzhou 510640 China Biomaterials Research Center College of Materials Science and Engineering South China University of Technology Guangzhou 510640 China

Micro-arc oxidation （MAO） is an enhanced chemical technology in an electrolyte medium to obtain coating structures on valve-metal surfaces. Titanium oxide films obtained by MAO in the sodium phosphate electrolyte were investigated. The films were composed mainly of TiO2 phases in the form of anatase and mille and enriched with Na and P elements at the surface. Their apafite-inducing ability was evaluated in a simulated body fluid （SBF）. When immersing in SBF for over 30 d, a preferential carbonated-hydroxyapatite was formed on the surfaces of the films, which suggests that the MAO-treated titanium has a promising positive biological response.

关键词： ceramic coatings micro-arc oxidation bio-mimetic mineralization carbonated-hydroxyapatite

3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone-implant interface in vivo

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第6期7卷 120-132页

作者： Xiao Liu Yali Miao Haifeng Liang Jingjing Diao Lijing Hao Zhifeng Shi naru zhao Yingjun Wang National Engineering Research Center for Tissue Restoration and Reconstruction GuangzhouChina School of Materials Science and Engineering South China University of TechnologyGuangzhou510641China Guangdong Province Key Laboratory of Biomedical Engineering South China University of TechnologyGuangzhou510006China Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education South China University of TechnologyGuangzhou510006PR China Innovation Center for Tissue Restoration and Reconstruction South China University of TechnologyGuangzhou510006PR China Department of Orthopedics Zhujiang HospitalSouthern Medical UniversityNo.253 Industrial AvenueHaizhuGuangzhou510280People’s Republic of China Medical Devices Research&Testing Center of SCUT Guangzhou510006PR China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) 510005 GuangzhouChina

Calcium phosphate bio-ceramics are osteo-conductive,but it remains a challenge to promote the induction of bone augmentation and capillary *** surface micro/nano-topography of materials can be recognized by cells and then the cell fate are *** regulation methods of carving surface structures on bio-ceramics employ mineral reagents and organic additives,which might introduce impurity phases and affect the biological *** a previous study,a facile and novel method was utilized with ultrapure water as the unique reagent for hydrothermal treatment,and a uniform hydroxyapatite(HAp)surface layer was constructed on composite ceramics(β-TCP/CaSiO_(3))in *** combined with 3D printing technology,biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramic scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface *** obtained HAp surface layer favoured cell adhesion,alleviated the cytotoxicity of precursor scaffolds,and upregulated the cellular differentiation of mBMSCs and gene expression of HUVECs in *** vivo studies showed that capillary formation,bone augmentation and new bone matrix formation were upregulated after the HAp surface layer was obtained,and the results confirmed that the fabricated biomimetic hierarchical structure scaffold could be an effective candidate for bone regeneration.

关键词： 3D printed porous scaffold Bioactive ceramics HAp surface layer Cell fate mediation Bone augmentation

3D printed pore morphology mediates bone marrow stem cell behaviors via RhoA/ROCK2 signaling pathway for accelerating bone regeneration

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第8期26卷 413-424页

作者： Qiji Lu Jingjing Diao Yingqu Wang Jianlang Feng Fansen Zeng Yan Yang Yudi Kuang naru zhao Yingjun Wang School of Materials Science and Engineering South China University of TechnologyGuangzhou510641PR China National Engineering Research Center for Tissue Restoration and Reconstruction South China University of TechnologyGuangzhou510006PR China NMPA Key Laboratory for Research and Evaluation of Innovative Biomaterials for Medical Devices.Guangzhou 510006PR China Medical Devices Research&Testing Center of SCUT Guangzhou510006PR China School of Biomedical Sciences and Engineering South China University of TechnologyGuangzhou International CampusGuangzhou511442PR China Guangdong Institute of Advanced Biomaterials and Medical Devices Guangzhou510535PR China

Bone bionics and structural engineering have sparked a broad interest in optimizing artificial scaffolds for better bone ***,the mechanism behind scaffold pore morphology-regulated bone regeneration remains unclear,making the structure design of scaffolds for bone repair *** address this issue,we have carefully assessed diverse cell behaviors of bone mesenchymal stem cells(BMSCs)on theβ-tricalcium phosphate(β-TCP)scaffolds with three representative pore morphologies(i.e.,cross column,diamond,and gyroid pore unit,respectively).Among the scaffolds,BMSCs on theβ-TCP scaffold with diamond pore unit(designated as D-scaffold)demonstrated enhanced cytoskeletal forces,elongated nucleus,faster cell mobility,and better osteogenic differentiation potential(for example,the alkaline phosphatase expression level in D-scaffold were 1.5-2 times higher than other groups).RNA-sequencing analysis and signaling pathway intervention revealed that Ras homolog gene family A(RhoA)/Rho-associated kinase-2(ROCK2)has in-depth participated in the pore morphology-mediated BMSCs behaviors,indicating an important role of mechanical signaling transduction in scaffold-cell ***,femoral condyle defect repair results showed that D-scaffold could effectively promote endogenous bone regeneration,of which the osteogenesis rate was 1.2-1.8 times higher than the other ***,this work provides insights into pore morphology-mediated bone regeneration mechanisms for developing novel bioadaptive scaffold designs.

关键词： Bone mesenchymal stem cells 3D-printed scaffold Pore morphology Bone regeneration Structure-osteogenesis relationship

Biomimetic hydroxyapatite coating on the 3D-printed bioactive porous composite ceramic scaffolds promoted osteogenic differentiation via PI3K/AKT/mTOR signaling pathways and facilitated bone regeneration in vivo

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2023年第5期136卷 54-64页

作者： Bizhi Tan naru zhao Wei Guo Fangli Huang Hao Hu Yan Chen Jungang Li Zemin Ling Zhiyuan Zou Rongcheng Hu Chun Liu Tiansheng Zheng Gang Wang Xiao Liu Yingjun Wang Xuenong Zou Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology Department of Spine SurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China School of Materials Science and Engineering South China University of TechnologyGuangzhou 510641China National Engineering Research Center for Tissue Restoration and Reconstruction Guangdong Province Key Laboratory of Biomedical EngineeringSouth China University of TechnologyGuangzhou 510006China Department of Orthopedics The First Affiliated Hospital of Gannan Medical UniversityGanzhou 341000China

The architecture and surface modifications have been regarded as effective methods to enhance the bi-ological response of biomaterials in bone tissue *** porous architecture of the implanta-tion was essential conditions for bone ***,the design of biomimetic hydroxyap-atite(HAp)coating on porous scaffolds was demonstrated to strengthen the bioactivity and stimulate ***,bioactive bio-ceramics such asβ-tricalcium phosphate(β-TCP)and calcium sili-cate(CS)with superior apatite-forming ability were reported to present better osteogenic activity than that of *** in this study,3D-printed interconnected porous bioactive ceramicsβ-TCP/CS scaf-fold was fabricated and the biomimetic HAp apatite coating were constructed in situ via hydrothermal reaction,and the effects of HAp apatite layer on the fate of mouse bone mesenchymal stem cells(mBM-SCs)and the potential mechanisms were *** results indicated that HAp apatite coating en-hanced cell proliferation,alkaline phosphatase(ALP)activity,and osteogenic gene ***-more,PI3K/AKT/mTOR signaling pathway is proved to have an important impact on cellular *** present results demonstrated that the key molecules of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT)and mammalian target of rapamycin(mTOR)were activated after the biomimetic hydrox-yapatite coating were constructed on the 3D-printed ceramic ***,the activated influence on the protein expression of Runx2 and BMP2 could be suppressed after the treatment of inhibitor *** vivo studies showed that the constructed HAp coating promoted bone formation and strengthen the bone *** results suggest that biomimetic HAp coating constructed on the 3D-printed bioac-tive composite scaffolds could strengthen the bioactivity and the obtained biomimetic multi-structured scaffolds might be a potential alternative bone graft for bone regeneration.

关键词： Bioactive ceramics Hydroxyapatite coating 3D-printed porous ceramic scaffold PI3K/AKT/mTOR signaling pathway Bone regeneration

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

纳米生物活性玻璃颗粒的制备及形态控制

复合材料学报 2011年第1期28卷 109-113页

作者：张娟娟雷波陈晓峰赵娜如林才华南理工大学生物医学工程研究院广州510640 教育部特种功能材料重点实验室广州510640 温州医学院附属第一医院温州325027

采用溶胶-凝胶技术制备纳米生物活性玻璃颗粒,通过在溶胶中加入柠檬酸来控制生物活性玻璃的形态结构。利用比表面积分析仪、透射电子显微镜和扫描电子显微镜对纳米生物活性玻璃的结构和形成机制进行了表征。研究结果表明:采用柠檬酸作... 详细信息

采用溶胶-凝胶技术制备纳米生物活性玻璃颗粒,通过在溶胶中加入柠檬酸来控制生物活性玻璃的形态结构。利用比表面积分析仪、透射电子显微镜和扫描电子显微镜对纳米生物活性玻璃的结构和形成机制进行了表征。研究结果表明:采用柠檬酸作为水解催化剂可以方便地控制生物活性玻璃的纳米结构形态,有利于制备较小粒径、大比表面积和孔体积的生物活性玻璃。这种制备技术提供了一种简单、低成本制备纳米生物活性玻璃并控制其纳米粒子形态的方法。

关键词：生物活性玻璃溶胶-凝胶法柠檬酸纳米颗粒

在线全文

学校读者我要写书评

暂无评论

梯度结构羟基磷灰石生物活性涂层的性能

材料研究学报 2006年第1期20卷 69-72页

作者：宁成云王迎军陈晓峰赵娜如华南理工大学材料学院

采用等离子喷涂系统在Ti6Al4V钛合金基体表面制备出具有梯度结构的羟基磷灰石生物活性梯度涂层,利用纳米硬度计等手段分析了生物活性涂层的梯度结构．结果表明:金属基体与陶瓷界面区域的弹性模量和硬度呈梯度变化;生物活性功能涂层的表... 详细信息

采用等离子喷涂系统在Ti6Al4V钛合金基体表面制备出具有梯度结构的羟基磷灰石生物活性梯度涂层,利用纳米硬度计等手段分析了生物活性涂层的梯度结构．结果表明:金属基体与陶瓷界面区域的弹性模量和硬度呈梯度变化;生物活性功能涂层的表面具有典型的多孔结构特征,整个涂层沿垂直基体方向从底层致密结构向表面层多孔结构过渡;涂层的成分从生物稳定性的底层至生物活性的表面层呈梯度变化,涂层表面成分为具有生物活性的羟基磷灰石．涂层的这种结构特征保持了涂层的生物活性, 提高涂层与基体的结合强度(48．6 MPa)．

关键词：无机非金属材料梯度涂层等离子喷涂生物材料

聚己内酯/纳米羟基磷灰石复合微球中的药物分布及体外释放(英文)

在线全文

学校读者我要写书评

暂无评论

硅酸盐学报 2008年第9期36卷 1225-1230页

作者：王旭东王迎军魏坤赵娜如张淑花华南理工大学材料科学与工程学院

采用单乳化溶剂挥发法制备了聚己内酯[poly(ε-caprolactone),PCL]/纳米羟基磷灰石(nano-hydroxyapatite,n-HA)复合微球。使用两种具有不同水溶性的模型药物对硝基苯胺(p-nitroaniline)和罗丹明B(Rhodamine B,RhB),研究n-HA在复合微球中的作用。用扫描电子显微镜观察微球的表面形貌。通过紫外–可见光分光光度法计算药物载量和包封率。用共聚焦激光显微镜分析药物在微球中的分布。分别研究了PCL微球和PCL/HA复合微球的体外释放性质。复合微球可以持续释放药物4周以上,在前3d的突释后,其释放曲线符合Higuchi扩散方程。n-HA的加入使较亲水药物RhB在复合微球中分布更均匀,对较疏水药物对硝基苯胺则影响不明显。n-HA减少了载亲水药物的复合微球在前3d的突释,并减缓了其后的释放速率。结果表明:PCL/n-HA复合结构的材料有希望作为一种新的长效药物释放载体应用。

关键词：聚己内酯纳米羟基磷灰石复合微球药物缓释