3D printed pore morphology mediates bone marrow stem cell behaviors via RhoA/ROCK2 signaling pathway for accelerating bone regeneration

作     者：Qiji Lu Jingjing Diao Yingqu Wang Jianlang Feng Fansen Zeng Yan Yang Yudi Kuang Naru Zhao Yingjun Wang 

作者机构：School of Materials Science and EngineeringSouth China University of TechnologyGuangzhou510641PR China National Engineering Research Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhou510006PR China NMPA Key Laboratory for Research and Evaluation of Innovative Biomaterials for Medical Devices.Guangzhou510006PR China Medical Devices Research&Testing Center of SCUTGuangzhou510006PR China School of Biomedical Sciences and EngineeringSouth China University of TechnologyGuangzhou International CampusGuangzhou511442PR China Guangdong Institute of Advanced Biomaterials and Medical DevicesGuangzhou510535PR China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第26卷第8期

页      面：413-424页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 

基　　金：support from the National key research and development plan(Grant No.2021YFB3800800) the National Natural Science Foundation of China(52172281) Natural Science Foundation of Guangdong Province(Grant No.2021A1515011741&No.2020A1515011354) the Science and Technology Program of Guangdong Province(Grant No.2019B010941002) the Science and Technology Program of Guangzhou(Grant No.202007020002&No.202206040001) the financial support from the National Natural Science Foundation of China(Grant No.52002132) China Postdoctoral Science Foundation(2019M662925) J.D.acknowledges the financial support from Guangzhou Science and Technology Planning Project(202102020005) National Natural Science Foundation of China(Grant No.32201089) Guangdong Science and Technology Planning Project(2022A1515010608) 

主　　题：Bone mesenchymal stem cells 3D-printed scaffold Pore morphology Bone regeneration Structure-osteogenesis relationship 

摘      要：Bone bionics and structural engineering have sparked a broad interest in optimizing artificial scaffolds for better bone ***,the mechanism behind scaffold pore morphology-regulated bone regeneration remains unclear,making the structure design of scaffolds for bone repair *** address this issue,we have carefully assessed diverse cell behaviors of bone mesenchymal stem cells(BMSCs)on theβ-tricalcium phosphate(β-TCP)scaffolds with three representative pore morphologies(i.e.,cross column,diamond,and gyroid pore unit,respectively).Among the scaffolds,BMSCs on theβ-TCP scaffold with diamond pore unit(designated as D-scaffold)demonstrated enhanced cytoskeletal forces,elongated nucleus,faster cell mobility,and better osteogenic differentiation potential(for example,the alkaline phosphatase expression level in D-scaffold were 1.5-2 times higher than other groups).RNA-sequencing analysis and signaling pathway intervention revealed that Ras homolog gene family A(RhoA)/Rho-associated kinase-2(ROCK2)has in-depth participated in the pore morphology-mediated BMSCs behaviors,indicating an important role of mechanical signaling transduction in scaffold-cell ***,femoral condyle defect repair results showed that D-scaffold could effectively promote endogenous bone regeneration,of which the osteogenesis rate was 1.2-1.8 times higher than the other ***,this work provides insights into pore morphology-mediated bone regeneration mechanisms for developing novel bioadaptive scaffold designs.