Biomimetic hydroxyapatite coating on the 3D-printed bioactive porous composite ceramic scaffolds promoted osteogenic differentiation via PI3K/AKT/mTOR signaling pathways and facilitated bone regeneration in vivo
作者机构：Guangdong Provincial Key Laboratory of Orthopaedics and TraumatologyDepartment of Spine SurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China School of Materials Science and EngineeringSouth China University of TechnologyGuangzhou 510641China National Engineering Research Center for Tissue Restoration and ReconstructionGuangdong Province Key Laboratory of Biomedical EngineeringSouth China University of TechnologyGuangzhou 510006China Department of OrthopedicsThe First Affiliated Hospital of Gannan Medical UniversityGanzhou 341000China
出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)
年 卷 期：2023年第136卷第5期
页 面：54-64页
核心收录：
学科分类：08[工学] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）]
基 金：This work was sponsored by the National Science Foundation of China(Nos.32071341,52202358,52003302) The Natural Science Foundation of Guangdong Province(No.2017A030308004) the Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110824) the Science and Technology Project of Guangdong province(No.2018A050506021)
主 题：Bioactive ceramics Hydroxyapatite coating 3D-printed porous ceramic scaffold PI3K/AKT/mTOR signaling pathway Bone regeneration
摘 要：The architecture and surface modifications have been regarded as effective methods to enhance the bi-ological response of biomaterials in bone tissue *** porous architecture of the implanta-tion was essential conditions for bone ***,the design of biomimetic hydroxyap-atite(HAp)coating on porous scaffolds was demonstrated to strengthen the bioactivity and stimulate ***,bioactive bio-ceramics such asβ-tricalcium phosphate(β-TCP)and calcium sili-cate(CS)with superior apatite-forming ability were reported to present better osteogenic activity than that of *** in this study,3D-printed interconnected porous bioactive ceramicsβ-TCP/CS scaf-fold was fabricated and the biomimetic HAp apatite coating were constructed in situ via hydrothermal reaction,and the effects of HAp apatite layer on the fate of mouse bone mesenchymal stem cells(mBM-SCs)and the potential mechanisms were *** results indicated that HAp apatite coating en-hanced cell proliferation,alkaline phosphatase(ALP)activity,and osteogenic gene ***-more,PI3K/AKT/mTOR signaling pathway is proved to have an important impact on cellular *** present results demonstrated that the key molecules of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT)and mammalian target of rapamycin(mTOR)were activated after the biomimetic hydrox-yapatite coating were constructed on the 3D-printed ceramic ***,the activated influence on the protein expression of Runx2 and BMP2 could be suppressed after the treatment of inhibitor *** vivo studies showed that the constructed HAp coating promoted bone formation and strengthen the bone *** results suggest that biomimetic HAp coating constructed on the 3D-printed bioac-tive composite scaffolds could strengthen the bioactivity and the obtained biomimetic multi-structured scaffolds might be a potential alternative bone graft for bone regeneration.
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