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Simultaneous optimal estimates of fixed effects and variance components in the mixed model

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Science China Mathematics 2004年第5期47卷 787-799页

作者： WU mixia wang Songgui College of Applied Sciences Beijing University of Technology Beijing 100022 College of Applied Sciences Beijing University of Technology Beijing 100022

For a general linear mixed model with two variance components, a set of simple conditions is obtained, under which, (i) the least squares estimate of the fixed effects and the analysis of variance (ANOVA) estimates of variance components are proved to be uniformly minimum variance unbiased estimates simultaneously; (ii) the exact confidence intervals of the fixed effects and uniformly optimal unbiased tests on variance components are given; (iii) the exact probability expression of ANOVA estimates of variance components taking negative value is obtained.

关键词： linear mixed model, random effect, variance component, minimum variance unbiased estimate,uniformly most powerful unbiased test.

A NEW QUANTITATIVE DETECTION METHOD OF RECOMBINANT CFP10-ESAT6 AMALGAMATION PROTEINS FROM MYCOBACTERIUM TUBERCULOSIS BASED ON MICRO-MAGNETIC PROBES STRATEGY

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Journal of Innovative Optical Health Sciences 2012年第1期5卷 55-60页

作者： YIQING HUANG JINPING LUO mixia wang JUNTAO LIU XINXIA CAI State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100190China Graduate School of Chinese Academy of Sciences Beijing 100190 China

A new rapid,specific and sensitive method for assay of recombinant CFP10-ESAT6 amalgamation proteins from Mycobacterium tuberculosis was *** method used streptavidincoated magnetic beads to enrich the specific biotinylated anti-CFP10 antibody,then adopted a sandwich-type enzyme linked immunosorbent assay technology with two kinds of monoclonal antibodies:biotinylated anti-CFP10 antibody and HRP-labeled anti-CFP10 antibody to identify the target CFP10-ESAT6 proteins,and finally detected chemiluminescence intensity by a small home-made optical *** was shown that,the corresponding chemiluminescence intensity had a good logarithmic linear response to the concentration of CFP10-ESAT6 proteins when ranging at 1~1000 ng/mL,and the correlation coefficient is *** proposed method could detect the CFP10-ESAT6 proteins with low detection limit(1 ng/mL)and the detection time could be controlled within 45 *** with commonly used detection methods of ***,this method was easy to operate,faster,and of higher *** achievement of the quantitative detection of CFP10-ESAT6 proteins has important scientific significance and wide application prospects in tuberculosis control.

关键词： Enzyme linked immunosorbent assay chemiluminescence home-made optical sensor

Recent advances and research progress on microsystems and bioeffects of terahertz neuromodulation

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Microsystems & Nanoengineering 2023年第6期9卷 25-41页

作者： Meiting Liu Juntao Liu Wei Liang Botao Lu Penghui Fan Yilin Song mixia wang Yirong Wu Xinxia Cai State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing100049China

Terahertz waves can interact with the nervous system of organisms under certain *** to common optical modulation methods,terahertz waves have the advantages of low photon energy and low risk;therefore,the use of terahertz waves to regulate the nervous system is a promising new method of ***,most of the research has focused on the use of terahertz technology for biodetection,while relatively little research has been carried out on the biological effects of terahertz radiation on the nervous system,and there are almost no review papers on this *** the present article,we begin by reviewing principles and objects of research regarding the biological effects of terahertz radiation and summarizing the current state of related research from a variety of aspects,including the bioeffects of terahertz radiation on neurons in vivo and in vitro,novel regulation and detection methods with terahertz radiation devices and neural microelectrode arrays,and theoretical simulations of neural information encoding and *** addition,we discuss the main problems and their possible causes and give some recommendations on possible future *** paper will provide insight and assistance to researchers in the fields of neuroscience,terahertz technology and biomedicine.

关键词： breakthrough neural microelectrode

Wireless closed-loop deep brain stimulation using microelectrode array probes

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第10期25卷 803-823页

作者： Qianli JIA Yaoyao LIU Shiya LV Yiding wang Peiyao JIAO Wei XU Zhaojie XU mixia wang Xinxia CAI State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China

Deep brain stimulation(DBS),including optical stimulation and electrical stimulation,has been demonstrated considerable value in exploring pathological brain activity and developing treatments for neural *** in DBS microsystems based on implantable microelectrode array(MEA)probes have opened up new opportunities for closed-loop DBS(CL-DBS)in *** technology can be used to detect damaged brain circuits and test the therapeutic potential for modulating the output of these circuits in a variety of diseases *** the success and rapid utilization of MEA probe-based CL-DBS microsystems,key challenges,including excessive wired communication,need to be urgently *** this review,we considered recent advances in MEA probe-based wireless CL-DBS microsystems and outlined the major issues and promising prospects in this *** technology has the potential to offer novel therapeutic options for psychiatric disorders in the future.

关键词： Deep brain stimulation(DBS) Wireless closed-loop deep brain stimulation(CL-DBS)microsystem Microelectrode array(MEA)probe Optical stimulation Electrical stimulation

Grid cell remapping under three-dimensional object and social landmarks detected by implantable microelectrode arrays for the medial entorhinal cortex

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Microsystems & Nanoengineering 2022年第5期8卷 171-181页

作者： Zhaojie Xu Fan Mo Gucheng Yang Penghui Fan Yiding wang Botao Lu Jingyu Xie Yuchuan Dai Yilin Song Enhui He Shihong Xu Juntao Liu mixia wang Xinxia Cai State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Grid cells with stable hexagonal firing patterns in the medial entorhinal cortex(MEC)carry the vital function of serving as a metric for the surrounding *** this mechanism processes only spatial information or involves nonspatial information remains ***,we fabricated an MEC-shaped microelectrode array(MEA)to detect the variation in neural spikes and local field potentials of the MEC when rats forage in a square enclosure with a planar,three-dimensional object and social landmarks in *** results showed that grid cells exhibited rate remapping under social conditions in which spike firing fields closer to the social landmark had a higher firing ***,global remapping showed that hexagonal firing patterns were rotated and scaled when the planar landmark was replaced with object and social *** addition,when grid cells were activated,the local field potentials were dominated by the theta band(5–8 Hz),and spike phase locking was observed at troughs of theta *** results suggest the pattern separation mechanism of grid cells in which the spatial firing structure and firing rate respond to spatial and social information,respectively,which may provide new insights into how the brain creates a cognitive map.

关键词： mapping hexagonal microelectrode

Real-time simultaneous recording of electrophysiological activities and dopamine overflow in the deep brain nuclei of a non-human primate with Parkinson’s disease using nano-based microelectrode arrays

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Microsystems & Nanoengineering 2018年第1期4卷 541-549页

作者： Song Zhang Yilin Song mixia wang Guihua Xiao Fei Gao Ziyue Li Guoxian Tao Ping Zhuang Feng Yue Piu Chan Xinxia Cai State Key Laboratory of Transducer Technology Institute of ElectronicsChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100190China Wincon TheraCells Biotechnologies Co. Ltd.Nanning 530002China Department of Neurobiology Xuanwu HospitalCapital Medical UniversityThe Beijing Key Laboratory of Parkinson's DiseaseBeijing 100053China

Parkinson’s disease(PD)is characterized by a progressive degeneration of nigrostriatal dopaminergic *** precise mechanisms are still *** the neuronal communications are inherently electrical and chemical in nature,dual-mode detection of PD-related neuroelectrical and neurochemical information is essential for PD *** nucleus(STN)highfrequency stimulation(HFS)can improve most symptoms of PD patients and decrease the dosage of antiparkinsonian *** mechanism of STN-HFS for PD still remains *** this study,a silicon-based dual-mode microelectrode array(MEA)probe was designed and fabricated,and systematic dual-mode detection methods were *** recording sites were modified using Pt nanoparticles and Nafion to improve the signal-to-noise(SNR)*** evaluate its applicability to PD research,in vivo electrophysiological and electrochemical detection was performed in normal and hemiparkinsonian models,*** comparison of the dual-mode signals,we demonstrated the following in a PD monkey:(1)the maximum dopamine concentration in the striatum decreased by 90%;(2)the spike firing frequency increased significantly,especially in the region of the cortex;(3)the spectrogram analysis showed that much power existed in the 0–10 Hz frequency band;and(4)following repeated subthalamic nucleus high-frequency stimulation trials,the level of DA in the striatum increased by 16.5μM,which led to a better elucidation of the mechanism of *** dual-mode MEA probe was demonstrated to be an effective tool for the study of neurological disorders.

关键词： dual mode implantable MEA non-human primate Parkinson's disease STN-HFS

Impaired Spatial Firing Representations of Neurons in the Medial Entorhinal Cortex of the Epileptic Rat Using Microelectrode Arrays

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Research 2024年第2期2023卷 541-552页

作者： Zhaojie Xu Fan Mo Gucheng Yang Penghui Fan Botao Lu Wei Liang Fanli Kong Luyi Jing Wei Xu Juntao Liu mixia wang Yirong Wu Xinxia Cai State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Epilepsy severely impairs the cognitive behavior of *** remains unclear whether epilepsy-induced cognitive impairment is associated with neuronal activities in the medial entorhinal cortex(MEC),a region known for its involvement in spatial *** explore this neural mechanism,we recorded the spikes and local field potentials from MEC neurons in lithium-pilocarpine-induced epileptic rats using self-designed microelectrode *** the open field test,we identified spatial cells exhibiting spatially selective firing properties and assessed their spatial representations in relation to the progression of ***,we analyzed theta oscillations and theta modulation in both excitatory and inhibitory ***,we used a novel object recognition test to evaluate changes in spatial cognitive ability of epileptic *** the epilepsy modeling,the spatial tuning of various types of spatial cells had suffered a rapid and pronounced damage during the latent period(1 to 5 d).Subsequently,the firing characteristics and theta oscillations were *** the chronic period(>10 d),the performance in the novel object experiment *** conclusion,our study demonstrates the detrimental effect on spatial representations and electrophysiological properties of MEC neurons in the epileptic latency,suggesting the potential use of these changes as a"functional biomarker"for predicting cognitive impairment caused by epilepsy.

关键词： epilepsy impaired involvement

Deep brain implantable microelectrode arrays for detection and functional localization of the subthalamic nucleus in rats with Parkinson’s disease

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Bio-Design and Manufacturing 2024年第4期7卷 439-452页

作者： Luyi Jing Zhaojie Xu Penghui Fan Botao Lu Fan Mo Ruilin Hu Wei Xu Jin Shan Qianli Jia Yuxin Zhu Yiming Duan mixia wang Yirong Wu Xinxia Cai State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China

The subthalamic nucleus(STN)is considered the best target for deep brain stimulation treatments of Parkinson’s disease(PD).It is difficult to localize the STN due to its small size and deep *** microelectrode arrays(MEAs)can rapidly and precisely locate the STN,which is important for precise *** this paper,16-channel MEAs modified with multiwalled carbon nanotube/poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate)(MWCNT/PEDOT:PSS)nanocomposites were designed and fabricated,and the accurate and rapid identification of the STN in PD rats was performed using detection sites distributed at different brain *** results showed that nuclei in 6-hydroxydopamine hydrobromide(6-OHDA)-lesioned brains discharged more intensely than those in unlesioned *** addition,the MEA simultaneously acquired neural signals from both the STN and the upper or lower boundary nuclei of the ***,higher values of spike firing rate,spike amplitude,local field potential(LFP)power,and beta oscillations were detected in the STN of the 6-OHDA-lesioned brain,and may therefore be biomarkers of STN *** with the STNs of unlesioned brains,the power spectral density of spikes and LFPs synchronously decreased in the delta band and increased in the beta band of 6-OHDA-lesioned *** may be a cause of sleep and motor disorders associated with ***,this work describes a new cellular-level localization and detection method and provides a tool for future studies of deep brain nuclei.

关键词： Functional localization Implantable microelectrode arrays Parkinson’s disease Subthalamic nucleus

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不同生育期调亏灌溉对温室梨枣品质的影响

农业工程学报 2009年第7期25卷 32-38页

作者：崔宁博杜太生李忠亭王密侠郭军西北农林科技大学旱区农业水土工程教育部重点实验室杨凌712100 四川大学水利水电学院成都610065 中国农业大学中国农业水问题研究中心北京100083 陕西省渭南市洛惠渠管理局渭南715100

为探明调亏灌溉对温室梨枣品质的影响,2005-2007年以日光温室7年生矮化密植梨枣树为试材进行试验,试验期间设置充分供水处理(即对照T1),萌芽展叶期(Ⅰ期)轻度或中度亏水处理(T2),及开花座果期(Ⅱ期)、果实膨大期(Ⅲ期)和果实成熟期(Ⅳ期... 详细信息

为探明调亏灌溉对温室梨枣品质的影响,2005-2007年以日光温室7年生矮化密植梨枣树为试材进行试验,试验期间设置充分供水处理(即对照T1),萌芽展叶期(Ⅰ期)轻度或中度亏水处理(T2),及开花座果期(Ⅱ期)、果实膨大期(Ⅲ期)和果实成熟期(Ⅳ期)中度亏水处理(即T3、T4和T5)。结果表明:不同生育期亏水处理对梨枣品质的影响差异明显;与对照相比,3a间Ⅰ期、Ⅱ期亏水处理对梨枣单果质量、含水率影响较小(P>0.05),梨枣硬度提高13.7%～15.8%,有机酸、坏果率分别下降14.2%～18.8%和21.0%～25.0%,且均达显著水平(PⅢ期>Ⅱ期≈Ⅰ期。综合考虑亏水处理对梨枣品质各项指标影响,果实成熟期中度亏水处理对品质改善效果最佳,具有重要推广价值。

关键词：调亏灌溉温室品质控制梨枣产量