检索结果-南通市图书馆

Reverse Genetic Approaches in Zebrafish

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2012年第9期39卷 421-433页

作者： Peng Huang Zuoyan Zhu shuo lin Bo Zhang Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education College of Life SciencesPeking UniversityBeijing 100871China Department of Molecular Cell&Developmental BiologyUniversity of CaliforniaLos AngelesCA 90095USA

Zebrafish（Danio rerio） is a well-established vertebrate animal model.A comprehensive collection of reverse genetics tools has been developed for studying gene function in this useful *** is the most widely used reagent to knock down target gene expression *** a long time,targeted genome modification has been heavily relied on large-scale traditional forward genetic screens,such as ENU（N-ethyl-N-nitrosourea） mutagenesis derived TILlinG（Targeting Induced Local Lesions IN Genomes） strategy and pseudo-typed retrovirus mediated insertional ***,engineered endonucleases,including ZFNs（zinc finger nucleases） and TALENs（transcription activator-like effector nucleases）,provide new and efficient strategies to directly generate site-specific indel mutations by inducing double strand breaks in target *** we summarize the major reverse genetic approaches for loss-of-function studies used and emerging in zebrafish,including strategies based on genome-wide mutagenesis and methods for site-specific gene *** directions and expectations will also be discussed.

关键词： Zebrafish Reverse genetics Morpholino TILlinG Retrovirus ZFN TALEN Gene targeting

DRY SLIDING WEAR OF PM Al-10Ti ALLOY

在线全文

学校读者我要写书评

暂无评论

中国有色金属学会会刊：英文版 1998年第3期8卷 66-71页

作者： Wu, Nianqiang Wu, Jinming lin, shuo Zhou, Jinqi Li, Zhizhang Zhejiang Univ Hangzhou China

１ＩＮＴＲＯＤＵＣＴＩＯＮＤｕｒｉｎｇｄｒｙｓｌｉｄｉｎｇｏｆｔｈｅａｌｕｍｉｎｕｍａｌｏｙｓａｎｄａｌｕｍｉｎｕｍｍａｔｒｉｘｃｏｍｐｏｓｉｔｅｓａｇａｉｎｓｔｓｔｅｅｌｓ，ｔｈｅｒｅｅｘｉｓｔｔｗｏｄｉｓｔｉｎｃ... 详细信息

１ＩＮＴＲＯＤＵＣＴＩＯＮＤｕｒｉｎｇｄｒｙｓｌｉｄｉｎｇｏｆｔｈｅａｌｕｍｉｎｕｍａｌｏｙｓａｎｄａｌｕｍｉｎｕｍｍａｔｒｉｘｃｏｍｐｏｓｉｔｅｓａｇａｉｎｓｔｓｔｅｅｌｓ，ｔｈｅｒｅｅｘｉｓｔｔｗｏｄｉｓｔｉｎｃｔｗｅａｒｒｅｇｉｍｅｓ，ｉ．ｅ．...

关键词： Al Ti alloy dry sliding wear delamination

Identification and expression analysis of mical family genes in zebrafish

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2010年第10期37卷 685-693页

作者： Yulin Xue Chikin Kuok An Xiao Zuoyan Zhu shuo lin Bo Zhang Key Laboratory of Cell Proliferation and Differentiation Center of Developmental Biology and Genetics College of Life Sciences Peking University Ministry of Education Beijing 100871 China Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095-1606 USA

Mical （molecule interacting with CasL） represent a conserved family of cytosolic multidomain proteins that has been shown to be as- sociated with a variety of cellular processes, including axon guidance, cell movement, cell-cell junction formation, vesicle trafficking and cancer cell metastasis. However, the expression and function of these genes during embryonic development have not been comprehen- sively characterized, especially in vertebrate species, although some limited in vivo studies have been carried out in neural and muscula- ture systems of Drosophila and in neural systems of vertebrates. So far, no mical family homologs have been reported in zebrafish, an ideal vertebrate model for the study of developmental processes. Here we report eight homologs of mical family genes in zebrafish and their expression profiles during embryonic development. Consistent with the findings in Drosophila and mammals, most zebra_fish mical family genes display expression in neural and musculature systems. In addition, five mical homologs are detected in heart, and one, mi- call2a, in blood vessels. Our data established an important basis for further functional studies of mical family genes in zebrafish, and suggest a possible role for mical genes in cardiovascular development.

关键词： mical micall2a expression pattern blood vessel heart

Characterization and expression pattern of pouII1,a novel class Ⅱ POU gene in zebrafish

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2001年第18期46卷 1523-1527,1586页

作者： LI Ming CAO Ying ZHAO Zhixing lin shuo MENG Anming Institute of Cellular and Developmental Biology Department of Biological Science and Biotechnologyand the Protein Science Laboratory of MOETsinghua UniversityBeijing 100084China Institute for Molecular Medicine and Genetics Medical College of GeorgiaGA30912USA

POU domain transcription factors that share a conserved DNA-binding domain, POU domain, are important regulators for the development of embryos in various animal species. A novel zebrafish POU domain gene, poulll has been cloned. The poullll cDNA is 2080 kb in length and encodes a putative polypeptide of 596 ammo acids. It is placed into class II POU family since it shares a high degree of homology with the known members of this family. Northern hybridization identifies a major transcript of approximately 2.1 kb that was present in embryos at the single-cell stage throughout 24 h postfertilization. The whole mount in situ hybridization shows that poulll transcripts are present in the single-cell embryos, strongly suggesting that these transcripts are of maternal origin. During early development of the embryos, poulll mRNA was ubiquitously distributed in all cells and tissues. The transcripts are gradually limited to brains and become completely undetectable by day 3. To our knowledge, poulll is the

关键词： POU zebrafish maternal gene embryogenesis embryos expression pattern.

Optimized Target-AID system efficiently induces single base changes in zebrafish

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2018年第4期45卷 215-217页

作者： Xiaochan Lu Yunxing Liu Guanrong Yan Song Li Wei Qin shuo lin State Key Laboratory of Chemical Oncogenomics Key Laboratory of Chemical Genomics Peking University Shenzhen Graduate School Department of Molecular Cell and Development Biology University of CaliforniaLos Angeles

CRISPR-Cas system has been widely adapted as a platform for genome editing in various eukaryotic organisms, including zebrafish（Cong et al., 2013; Hwang et al., 2013）. One of the important applications of CRISPR-Cas... 详细信息

关键词： AID Optimized Target-AID system efficiently induces single base changes in zebrafish Target

ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

在线全文

学校读者我要写书评

暂无评论

Cell Regeneration 2015年第1期4卷 1-7页

作者： Anne G lindgren Matthew B Veldman shuo lin Department of Molecular Cellular and Developmental Biology University of California615 Charles E.Young Drive SouthLos AngelesCA 90095USA

Background:Endothelial cells line the luminal surface of blood vessels and form a barrier between the blood and other tissues of the *** variant 2(ETV2)is transiently expressed in both zebrafish and mice and is necessary and sufficient for vascular endothelial cell *** of this gene in early zebrafish and mouse embryos results in ectopic appearance of endothelial *** expression of ETV2 in later development results in only a subset of cells responding to the ***:We have examined the expression pattern of ETV2 in differentiating human embryonic stem cells(ESCs)to determine when the peak of ETV2 expression *** show that overexpression of ETV2 in differentiating human ESC is able to increase the number of endothelial cells generated when administered during or after the endogenous peak of gene ***:Addition of exogenous ETV2 to human ESCs significantly increased the number of cells expressing angioblast genes without arterial or venous *** may be a viable solution to generate in vitro endothelial cells for use in research and in the clinic.

关键词： Human embryonic stem cells Endothelium ETV2 Differentiation

Local chemical ordering coordinated thermal stability of nanograined high-entropy alloys

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rare Metals 2023年第5期42卷 1645-1655页

作者： Hong-Hui Wu lin-shuo Dong Shui-Ze Wang Gui-lin Wu Jun-Heng Gao Xu-Sheng Yang Xiao-Ye Zhou Xin-Ping Mao Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing100083China Yangjiang Branch Guangdong Laboratory for Materials Science and Technology(Yangjiang Advanced Alloys Laboratory)Yangjiang529500China Department of Industrial and Systems Engineering The Hong Kong Polytechnic UniversityKowloon999077Hong KongChina Guangdong Province Key Laboratory of Durability for Marine Civil Engineering School of Civil EngineeringShenzhen UniversityShenzhen518060China

Nanograined(NG)materials often suffer from low thermal stability owing to the high volume fraction of grain boundaries(GBs).Herein,we investigate the possibility of utilizing local chemical ordering(LCO)for improving the thermal stability of NG FeCoNiCrMn highentropy alloys(HE As).NG HE As with two different grain sizes were *** tests and creep test simulations were then performed to reveal the influence of LCO on the mechanical properties and thermal stability of NG HE *** performing hybrid molecular dynamics and Monte Carlo simulations,Cr atoms were found to accumulate at *** analyzing the atomic structure evolution during the deformation process,we found that the formation of LCO effectively stabilized the GBs and inhibited GB *** addition,dislocation nucleation from GBs and dislocation movement was also *** inhibiting effect of LCO on GB movement and dislocation activity is more prominent than in the NG model with smaller grain *** current simulation results suggest a possible strategy for enhancing the thermal stability of NG HEAs for service in a high-temperature environment.

关键词： High-entropy alloys(HEAs) Local chemical ordering(LCO) Molecular dynamics(MD)simulation Monte Carlo(MC)approach

Association of serum lipoprotein(a) level with the severity and prognosis of calcific aortic valve stenosis: a Chinese cohort study

在线全文

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2020年第3期17卷 133-140页

作者： shuo-lin LIU Rynat Rozi Hui-Wei SHI Ying GAO Yuan-lin GUO Yi-Da TANG Jian-Jun LI Na-Qiong WU Endocrinology&Cardiometabolic Center State Key Laboratory of Cardiovascular DiseaseFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Background There was a causal relationship between elevated lipoprotein(a)[Lp(a)]levels and increased risk of calcific aortic valve stenosis(CAVS)in whites and *** present study aimed to investigate whether Lp(a)levels were associated with aortic stenosis(AS)severity and clinical events in Chinese *** Levels of serum Lp(a)were measured in 652 patients with CAVS,whom all underwent baseline echocardiographic *** clinical endpoint was defined as a composite of aortic valve replacement(AVR)and cardiac *** Patients in the tertile 3 of Lp(a)had a higher percentage of severe AS compared with those in the tertile 1 and 2 of Lp(a)(46.2%vs.33.9%,P=0.005).Moreover,the top tertile of Lp(a)was an independent predictor of severe AS(OR=1.78,95%CI:1.18-2.66,P=0.006).However,there was no significant association between tertile 3 of Lp(a)and clinical events(hazard ratio:0.73;95%CI:0.43-1.24;P=0.239)in the multivariate Cox regression analysis during a mean follow-up time of 3.16±2.74 *** Elevated Lp(a)level was an independent predictor of severe AS by echocardiography in the Chinese population,but was not associated with the increased risk of AVR and cardiac death,suggesting that Lp(a)levels might be helpful in the risk stratification of patients with CAVS.

关键词： Aortic stenosis Calcification Severity Lipoprotein(a) Prognosis

A Zebrafish Model of 5q-Syndrome Using CRISPR/Cas9 Targeting RPS14 Reveals a p53-Independent and p53-Dependent Mechanism of Erythroid Failure

在线全文

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2016年第5期43卷 307-318页

作者： Jason Ear Jessica Hsueh Melinda Nguyen QingHua Zhang Victoria Sung Rajesh Chopra Kathleen M.Sakamoto shuo lin Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095 USA Celgene Corporation San Francisco 94158 USA Celgene Corporation Summit NJ 07901 USA Division of Hematology/Oncology Department of Pediatrics Stanford University School of Medicine Stanford CA 94304 USA

5q-syndrome is a distinct form of myelodysplastic syndrome（MDS） where a deletion on chromosome 5 is the underlying *** is characterized by bone marrow failures,including macrocytic *** mapping and studies using various models support the notion that ribosomal protein S14（RPS14） is the candidate gene for the erythroid *** disruption of RPS14 causes an increase in p53 activity and p53-mediated apoptosis,similar to what is observed with other ribosomal ***,due to the higher risk for cancer development in patients with ribosome deficiency,targeting the p53 pathway is not a viable treatment *** better understand the pathology of RPS14 deficiency in 5q-deletion,we generated a zebrafish model harboring a mutation in the RPS14 *** model mirrors the anemic phenotype seen in ***,the anemia is due to a late-stage erythropoietic defect,where the erythropoietic defect is initially p53-independent and then becomes ***,we demonstrate the versatility of this model to test various pharmacological agents,such as RAP-011,L-leucine,and dexamethasone in order to identify molecules that can reverse the anemic phenotype.

关键词： 5q-syndrome RPS14 Ribosomopathy Myelodysplastic syndrome