Optimized Target-AID system efficiently induces single base changes in zebrafish

作     者：Xiaochan Lu Yunxing Liu Guanrong Yan Song Li Wei Qin Shuo Lin 

作者机构：State Key Laboratory of Chemical Oncogenomics Key Laboratory of Chemical Genomics Peking University Shenzhen Graduate School Department of Molecular Cell and Development Biology University of CaliforniaLos Angeles 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2018年第45卷第4期

页      面：215-217页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0835[工学-软件工程] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 081202[工学-计算机软件与理论] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：supported by the funding from the Science and Technology Program of Shenzhen (JCYJ20150924110425180 and JCYJ20151030170755264) 

主　　题：AID Optimized Target-AID system efficiently induces single base changes in zebrafish Target 

摘      要：CRISPR-Cas system has been widely adapted as a platform for genome editing in various eukaryotic organisms, including zebrafish（Cong et al., 2013; Hwang et al., 2013）. One of the important applications of CRISPR-Cas9 system is to produce double-strand DNA breaks（DSBs） at targeted sites with guide RNA（gRNA）.