Characterization and expression pattern of pouII1,a novel class Ⅱ POU gene in zebrafish

作     者：LI Ming CAO Ying ZHAO Zhixing LIN Shuo MENG Anming 

作者机构：Institute of Cellular and Developmental BiologyDepartment of Biological Science and Biotechnologyand the Protein Science Laboratory of MOETsinghua UniversityBeijing 100084China Institute for Molecular Medicine and GeneticsMedical College of GeorgiaGA30912USA 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2001年第46卷第18期

页      面：1523-1527,1586页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 

基　　金：This work was supported in part by the National Key Basic Research and Development Program (Grant No. G19990756) the National Natural Science Foundation of China (Grant Nos. 30025020 and 39970360) and the "985" Program 

主　　题：POU zebrafish maternal gene embryogenesis embryos expression pattern. 

摘      要：POU domain transcription factors that share a conserved DNA-binding domain, POU domain, are important regulators for the development of embryos in various animal species. A novel zebrafish POU domain gene, poulll has been cloned. The poullll cDNA is 2080 kb in length and encodes a putative polypeptide of 596 ammo acids. It is placed into class II POU family since it shares a high degree of homology with the known members of this family. Northern hybridization identifies a major transcript of approximately 2.1 kb that was present in embryos at the single-cell stage throughout 24 h postfertilization. The whole mount in situ hybridization shows that poulll transcripts are present in the single-cell embryos, strongly suggesting that these transcripts are of maternal origin. During early development of the embryos, poulll mRNA was ubiquitously distributed in all cells and tissues. The transcripts are gradually limited to brains and become completely undetectable by day 3. To our knowledge, poulll is the