ETV2 expression increases the efficiency of primitive endothelial cell derivation from human embryonic stem cells

作     者：Anne G Lindgren Matthew B Veldman Shuo Lin 

作者机构：Department of Molecular Cellular and Developmental BiologyUniversity of California615 Charles E.Young Drive SouthLos AngelesCA 90095USA 

出 版 物：《Cell Regeneration》 (细胞再生（英文）)

年 卷 期：2015年第4卷第1期

页      面：1-7页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学] 

基　　金：The UCLA vector core is supported by JCCC/P30 CA016042 and CURE/P30 DK041301 The cells were supplied through the UCLA BSCRC stem cell core laboratory This work was supported by funds from the California Institute for Regenerative Medicine(CIRM RB3-02165) 

主　　题：Human embryonic stem cells Endothelium ETV2 Differentiation 

摘      要：Background:Endothelial cells line the luminal surface of blood vessels and form a barrier between the blood and other tissues of the *** variant 2(ETV2)is transiently expressed in both zebrafish and mice and is necessary and sufficient for vascular endothelial cell *** of this gene in early zebrafish and mouse embryos results in ectopic appearance of endothelial *** expression of ETV2 in later development results in only a subset of cells responding to the ***:We have examined the expression pattern of ETV2 in differentiating human embryonic stem cells(ESCs)to determine when the peak of ETV2 expression *** show that overexpression of ETV2 in differentiating human ESC is able to increase the number of endothelial cells generated when administered during or after the endogenous peak of gene ***:Addition of exogenous ETV2 to human ESCs significantly increased the number of cells expressing angioblast genes without arterial or venous *** may be a viable solution to generate in vitro endothelial cells for use in research and in the clinic.