检索结果-南通市图书馆

A self-fused hydrogel for the treatment of glottic insufficiency through outstanding durability,extracellular matrix-inducing bioactivity and function preservation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第6期24卷 54-68页

作者： Chen-Yu Zou Juan-Juan Hu Dan Lu qian-Jin Li Yan-Lin Jiang Rui Wang Hai-Yang Wang Xiong-Xin Lei Jesse Li-Ling hui Yang hui-qi xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041PR China Department of Otolaryngology Head and Neck SurgeryWest China HospitalSichuan UniversityChengduSichuan610041PR China Department of Medical Genetics West China Second HospitalSichuan UniversityChengduSichuan610041PR China

Injection laryngoplasty with biomaterials is an effective technique to treat glottic insufficiency.However,the inadequate durability,deficient pro-secretion of extracellular matrix(ECM)and poor functional preservation of current biomaterials have yielded an unsatisfactory therapeutic effect.Herein,a self-fusing bioactive hydrogel comprising modified carboxymethyl chitosan and sodium alginate is developed through a dual-crosslinking mechanism(photo-triggered and dynamic covalent bonds).Owing to its characteristic networks,the synergistic effect of the hydrogel for vocal folds(VFs)vibration and phonation is adequately demonstrated.Notably,owing to its inherent bioactivity of polysaccharides,the hydrogel could significantly enhance the secretion of major components(type I/III collagen and elastin)in the lamina propria of the VFs both in vivo and in vitro.In a rabbit model for glottic insufficiency,the optimized hydrogel(C1A1)has demonstrated a durability far superior to that of the commercially made hyaluronic acid(HA)Gel.More importantly,owing to the ECM-inducing bioactivity,the physiological functions of the VFs treated with the C1A1 hydrogel also outperformed that of the HA Gel,and were similar to those of the normal VFs.Taken together,through a simple-yet-effective strategy,the novel hydrogel has demonstrated outstanding durability,ECM-inducing bioactivity and physiological function preservation,therefore has an appealing clinical value for treating glottic insufficiency.

关键词： Glottic insufficiency Self-fusion Hydrogel Extracellular matrix Physiological function

Progress in development of bioderived materials for dermal wound healing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2017年第5期4卷 325-334页

作者： Lin-Cui Da Yi-Zhou Huang hui-qi xie Laboratory of Stem Cell and Tissue Engineering State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041People’s Republic of China

Treatment of acute and chronic wounds is one of the primary challenges faced by doctors.Bioderived materials have significant potential clinical value in tissue injury treatment and defect reconstruction.Various strategies,including drug loading,addition of metallic element(s),crosslinking and combining two or more distinct types of materials with complementary features,have been used to synthesize more suitable materials for wound healing.In this review,we describe the recent developments made in the processing of bioderived materials employed for cutaneous wound healing,including newly developed materials such as keratin and soy protein.The focus was on the key properties of the bioderived materials that have shown great promise in improving wound healing,restoration and reconstruction.With their good biocompatibility,nontoxic catabolites,microinflammation characteristics,as well as their ability to induce tissue regeneration and reparation,the bioderived materials have great potential for skin tissue repair.

关键词： wound healing tissue-engineered skin bioderived materials acellular matrix

Multi-crosslinking hydrogels with robust bio-adhesion and pro-coagulant activity for first-aid hemostasis and infected wound healing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第10期7卷 388-402页

作者： Chen-Yu Zou Xiong-Xin Lei Juan-Juan Hu Yan-Lin Jiang qian-Jin Li Yu-Ting Song qing-Yi Zhang Jesse Li-Ling hui-qi xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu610041PR China Department of Otolaryngology Head and Neck SurgeryWest China HospitalSichuan UniversityChengdu610041PR China Department of Medical Genetics West China Second HospitalSichuan UniversityChengdu610041PR China

Bio-adhesive polysaccharide-based hydrogels have attracted much attention in first-aid hemostasis and wound healing for excellent biocompatibility,antibacterial property and pro-healing bioactivity.Yet,the inadequate mechanical properties and bio-adhesion limit their applications.Herein,based on dynamic covalent bonds,photo-triggered covalent bonds and hydrogen bonds,multifunctional bio-adhesive hydrogels comprising modified carboxymethyl chitosan,modified sodium alginate and tannic acid are developed.Multi-crosslinking strategy endows hydrogels with improved strength and flexibility simultaneously.Owing to cohesion enhancement strategy and self-healing ability,considerable bio-adhesion is presented by the hydrogel with a maximal adhesion strength of 162.6 kPa,12.3-fold that of commercial fibrin glue.Based on bio-adhesion and pro-coagulant activity(e.g.,the stimulative aggregation and adhesion of erythrocytes and platelets),the hydrogel reveals superior hemostatic performance in rabbit liver injury model with blood loss of 0.32 g,only 54.2%of that in fibrin glue.The healing efficiency of hydrogel for infected wounds is markedly better than commercial EGF Gel and Ag+Gel due to the enhanced antibacterial and antioxidant properties.Through the multi-crosslinking strategy,the hydrogels show enhanced mechanical properties,fabulous bio-adhesion,superior hemostatic performance and promoting healing ability,thereby have an appealing application value for the first-aid hemostasis and infected wound healing.

关键词： Bio-adhesion First-aid hemostasis Infected wound healing Polysaccharide-based hydrogel Tannic acid

Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第8期7卷 206-218页

作者： Long-Mei Zhao Long Wang Wen-qian Zhang Rui Wang Xiu-Zhen Zhang Xiong-Xin Lei Yan Liang Yu-Ting Song qing-Yi Zhang Ke Lin hui-qi xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041China Department of Cardiovascular Surgery West China HospitalSichuan UniversityChengduSichuan610041China Department of Cardiovascular Surgery Shandong Provincial HospitalShandong First Medical UniversityJinanShandong250021China Research Core Facility West China HospitalSichuan UniversityChengduSichuan610041China

Approximately 25%of patients with congenital heart disease require implantation of patches to repair.However,most of the currently available patches are made of inert materials with unmatched electrical conductivity and mechanical properties,which may lead to an increased risk for arrhythmia and heart failure.In this study,we have developed a novel Polyurethane/Small intestinal submucosa patch(PSP)with mechanical and electrical properties similar to those of the native myocardial tissue,and assessed its feasibility for the reconstruction of right ventricular outflow tract.A right ventricular outflow tract reconstruction model was constructed in 40 rabbits.Compared with commercially available bovine pericardium patch,the PSP patch has shown better histocompatibility and biodegradability,in addition with significantly improved cardiac function.To tackle the significant fibrosis and relatively poor vascularization during tissue remodeling,we have further developed a bioactive patch by incorporating the PSP composites with urine-derived stem cells(USCs)which were pretreated with hypoxia.The results showed that the hypoxia-pretreated bioactive patch could significantly inhibit fibrosis and promote vascularization and muscularization,resulting in better right heart function.Our findings suggested that the PSP patch combined with hypoxia-pretreated USCs may provide a better strategy for the treatment of congenital heart disease.

关键词： Congenital heart disease Polypyrrole Polyurethane/small intestinal submucosa Hypoxia Urine-derived stem cells

Multifunctional two-component in-situ hydrogel for esophageal submucosal dissection for mucosa uplift,postoperative wound closure and rapid healing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第9期27卷 461-473页

作者： Xiong-Xin Lei Juan-Juan Hu Chen-Yu Zou Yan-Lin Jiang Long-Mei Zhao Xiu-Zhen Zhang Ya-Xing Li An-Ni Peng Yu-Ting Song Li-Ping Huang Jesse Li-Ling hui-qi xie Department of Orthopedic Surgery and Orthopedic Research Institute Laboratory of Stem Cell and Tissue EngineeringState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041China Department of Otolaryngology Head and Neck SurgeryWest China HospitalSichuan UniversityChengduSichuan610041China Institute of Biomedical Engineering and Informatics Ilmenau University of TechnologyGustav-Kirchh Off Str.298693IlmenauGermany Department of Medical Genetics West China Second HospitalSichuan UniversityChengduSichuan610041China

Endoscopic submucosal dissection (ESD) for gastrointestinal tumors and premalignant lesions needs submucosal fluid cushion (SFC) for mucosal uplift before dissection, and wound care including wound closure and rapid healing postoperatively. Current SFC materials as well as materials and/or methods for post-ESD wound care have single treatment effect and hold corresponding drawbacks, such as easy dispersion, short duration, weak hemostasis and insufficient repair function. Thus, designing materials that can serve as both SFC materials and wound care is highly desired, and remains a challenge. Herein, we report a two-component in-situ hydrogel prepared from maleimide-based oxidized sodium alginate and sulfhydryl carboxymethyl-chitosan, which gelated mainly based on "click" chemistry and Schiff base reaction. The hydrogels showed short gelation time, outstanding tissue adhesion, favorable hemostatic properties, and good biocompatibility. A rat subcutaneous ultrasound model confirmed the ability of suitable mucosal uplift height and durable maintenance time of AM solution. The in vivo/in vitro rabbit liver hemorrhage model demonstrated the effects of hydrogel in rapid hemostasis and prevention of delayed bleeding. The canine esophageal ESD model corroborated that the in-situ hydrogel provided good mucosal uplift and wound closure effects, and significantly accelerated wound healing with accelerating re-epithelization and ECM remodeling post-ESD. The two-component in-situ hydrogels exhibited great potential in gastrointestinal tract ESD.

关键词： Hydrogel Endoscopic submucosal dissection Mucosa uplift Tissue sealant Wound healing

Application of metabolomics in urolithiasis:the discovery and usage of succinate

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第2期8卷 771-783页

作者： Xiu-zhen Zhang Xiong-xin Lei Yan-lin Jiang Long-mei Zhao Chen-yu Zou Yun-jin Bai Ya-xing Li Rui Wang qian-jin Li qiu-zhu Chen Ming-hui Fan Yu-ting Song Wen-qian Zhang Yi Zhang Jesse Li-Ling hui-qi xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteDepartment of OrthopedicsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan 610041China Department of Urology Institute of UrologyWest China HospitalSichuan UniversityChengduSichuan 610041China Research Core Facility of West China Hospital Sichuan UniversityChengduSichuan 610041China Department of Medical Genetics West China Second University HospitalChengduSichuan 610041China

Urinary stone is conceptualized as a chronic metabolic disorder punctuated by symptomatic stone events.It has been shown that the occurrence of calcium oxalate monohydrate(COM)during stone formation is regulated by crystal growth modifiers.Although crystallization inhibitors have been recognized as a therapeutic modality for decades,limited progress has been made in the discovery of effective modifiers to intervene with stone disease.In this study,we have used metabolomics technologies,a powerful approach to identify biomarkers by screening the urine components of the dynamic progression in a bladder stone model.By in-depth mining and analysis of metabolomics data,we have screened five differential metabolites.Through density functional theory studies and bulk crystallization,we found that three of them(salicyluric,gentisic acid and succinate)could effectively inhibit nucleation in vitro.We thereby assessed the impact of the inhibitors with an EG-induced rat model for kidney stones.Notably,succinate,a key player in the tricarboxylic acid cycle,could decrease kidney calcium deposition and injury in the model.Transcriptomic analysis further showed that the protective effect of succinate was mainly through anti-inflammation,inhibition of cell adhesion and osteogenic differentiation.These findings indicated that succinate may provide a new therapeutic option for urinary stones.

关键词： crystallization thereby protective

Accelerating ESD-induced gastric ulcer healing using a pH-responsive polyurethane/small intestinal submucosa hydrogel delivered by endoscopic catheter

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2021年第1期8卷 112-123页

作者： Long-Mei Zhao Mei Gong Rui Wang qi-Juan Yuan Yi Zhang Jin-Kui Pi Xiu-He Lv Yan xie hui-qi xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyKeyuan Road 4Gaopeng Street ChengduSichuan 610041China Research Core Facility West China HospitalSichuan UniversityKeyuan Road 4Gaopeng StreetChengduSichuan 610041China Department of Gastroenterology West China HospitalSichuan UniversityNo.37 Guo Xue XiangChengduSichuan 610041China

Endoscopic submucosal dissection(ESD)is the standard treatment for early-stage gastric cancer,but the large post-operative ulcers caused by ESD often lead to serious side effects.Post-ESD mu-cosal repair materials provide a new option for the treatment of post-ESD ulcers.In this study,we developed a polyurethane/small intestinal submucosa(PU/SIS)hydrogel and investigated its effi-cacy for accelerating ESD-induced ulcer healing in a canine model.PU/SIS hydrogel possessed great biocompatibility and distinctive pH-sensitive swelling properties and protected GES-1 cells from acid attack through forming a dense film in acidic conditions in vitro.Besides,PU/SIS gels present a strong bio-adhesion to gastric tissues under acidic conditions,thus ensuring the reten-tion time of PU/SIS gels in vivo.In a canine model,PU/SIS hydrogel was easily delivered via endoscopy and adhered to the ulcer sites.PU/SIS hydrogel accelerated gastric ulcer healing at an early stage with more epithelium regeneration and slight inflammation.Our findings reveal PU/SIS hydrogel is a promising and attractive candidate for ESD-induced ulcer repair.

关键词： ESD-induced ulc er small inte stinal submucosa polyurethane bio-a dhesion pH-sensitive

Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2022年第8期7卷 443-455页

作者： Yu-Ting Song Yan-qing Li Mao-Xuan Tian Jun-Gen Hu Xiu-Ru Zhang Peng-Cheng Liu Xiu-Zhen Zhang qing-Yi Zhang Li Zhou Long-Mei Zhao Jesse Li-Ling hui-qi xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041China Department of Aesthetic Surgery The People’s Hospital of PengzhouChengduSichuan611930China Surgery of Spine and Spinal Cord Henan Provincial People’s HospitalZhengzhouHenan450000China Department of Burn and Plastic Surgery West China HospitalSichuan UniversityChengduSichuan610041China Research Core Facility of West China Hospital Sichuan UniversityChengduSichuan610041China Department of Medical Genetics and Prenatal Diagnosis West China Second HospitalSichuan UniversityChengduSichuan610041China

The need for bladder reconstruction and side effects of cystoplasty have spawned the demand for the development of alternative material substitutes.Biomaterials such as submucosa of small intestine(SIS)have been widely used as patches for bladder repair,but the outcomes are not fully satisfactory.To capture stem cells in situ has been considered as a promising strategy to speed up the process of re-cellularization and functionalization.In this study,we have developed an anti-CD29 antibody-conjugated SIS scaffold(AC-SIS)which is capable of specifically capturing urine-derived stem cells(USCs)in situ for tissue repair and regeneration.The scaffold has exhibited effective capture capacity and sound biocompatibility.In vivo experiment proved that the AC-SIS scaffold could promote rapid endothelium healing and smooth muscle regeneration.The endogenous stem cell capturing scaffolds has thereby provided a new revenue for developing effective and safer bladder patches.

关键词： Regenerative medicine Bladder tissue engineering Scaffold Antibody-conjugated

Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2020年第5期63卷 712-723页

作者： An-Jing Chen Jin-Kui Pi Jun-Gen Hu Yi-Zhou Huang Hong-Wei Gao Sheng-Fu Li Jesse Li-Ling hui-qi xie Laboratory of Stem Cell and Tissue Engineering State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041China Key Laboratory of Transplant Engineering and Immunology of Ministry of Health West China HospitalSichuan UniversityChengdu 610041China

Urine-derived stem cells(USCs)have shown potentials for the treatment of skeletal and urological disorders.Based on published literature and our own data,USCs consist of heterogeneous populations of cells.In this paper,we identify and characterize two morphologically distinct subpopulations of USCs from human urine samples,named as spindle-shaped USCs(SS-USCs)and rice-shaped USCs(RS-USCs)respectively.The two subpopulations showed similar clone-forming efficiency,while SS-USCs featured faster proliferation,higher motility,and greater potential for osteogenic and adipogenic differentiation,RS-USCs showed greater potential for chondrogenic differentiation.POU5F1 was strongly expressed in both subpopulations,but MYC was weakly expressed.Both subpopulations showed similar patterns of CD24,CD29,CD34,CD44,CD73,CD90 and CD105 expression,while a higher percentage of RS-USCs were positive for CD133.SS-USCs were positive for VIM,weakly positive for SLC12A1 and UMOD,and negative for KRT18,NPHS1,AQP1 and AQP2,indicating a renal mesenchyme origin;while RSUSCs are positive for VIM,partially positive for KRT18,NPHS1,AQP1,SLC12A1 and UMOD,and negative for AQP2,indicating a nephron tubule origin.The above results can facilitate understanding of the biological characteristics of subpopulations of USCs,and provide a basis for further research and applications of such cells.

关键词： characterization cell subpopulation identification tissue origin urine-derived stem cells