Multifunctional two-component in-situ hydrogel for esophageal submucosal dissection for mucosa uplift,postoperative wound closure and rapid healing

作     者：Xiong-Xin Lei Juan-Juan Hu Chen-Yu Zou Yan-Lin Jiang Long-Mei Zhao Xiu-Zhen Zhang Ya-Xing Li An-Ni Peng Yu-Ting Song Li-Ping Huang Jesse Li-Ling Hui-Qi Xie 

作者机构：Department of Orthopedic Surgery and Orthopedic Research InstituteLaboratory of Stem Cell and Tissue EngineeringState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041China Department of OtolaryngologyHead and Neck SurgeryWest China HospitalSichuan UniversityChengduSichuan610041China Institute of Biomedical Engineering and InformaticsIlmenau University of TechnologyGustav-Kirchh Off Str.298693IlmenauGermany Department of Medical GeneticsWest China Second HospitalSichuan UniversityChengduSichuan610041China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第27卷第9期

页      面：461-473页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 07[理学] 070304[理学-物理化学(含∶化学物理)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.32171351) the“1.3.5”Project for Disciplines of Excellence,West China Hospital,Sichuan University(Grant No.ZYJC18002) China Postdoctoral Science Foundation(2022M722277). 

主　　题：Hydrogel Endoscopic submucosal dissection Mucosa uplift Tissue sealant Wound healing 

摘      要：Endoscopic submucosal dissection (ESD) for gastrointestinal tumors and premalignant lesions needs submucosal fluid cushion (SFC) for mucosal uplift before dissection, and wound care including wound closure and rapid healing postoperatively. Current SFC materials as well as materials and/or methods for post-ESD wound care have single treatment effect and hold corresponding drawbacks, such as easy dispersion, short duration, weak hemostasis and insufficient repair function. Thus, designing materials that can serve as both SFC materials and wound care is highly desired, and remains a challenge. Herein, we report a two-component in-situ hydrogel prepared from maleimide-based oxidized sodium alginate and sulfhydryl carboxymethyl-chitosan, which gelated mainly based on click chemistry and Schiff base reaction. The hydrogels showed short gelation time, outstanding tissue adhesion, favorable hemostatic properties, and good biocompatibility. A rat subcutaneous ultrasound model confirmed the ability of suitable mucosal uplift height and durable maintenance time of AM solution. The in vivo/in vitro rabbit liver hemorrhage model demonstrated the effects of hydrogel in rapid hemostasis and prevention of delayed bleeding. The canine esophageal ESD model corroborated that the in-situ hydrogel provided good mucosal uplift and wound closure effects, and significantly accelerated wound healing with accelerating re-epithelization and ECM remodeling post-ESD. The two-component in-situ hydrogels exhibited great potential in gastrointestinal tract ESD.