A self-fused hydrogel for the treatment of glottic insufficiency through outstanding durability,extracellular matrix-inducing bioactivity and function preservation
作者机构：Laboratory of Stem Cell and Tissue EngineeringOrthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041PR China Department of OtolaryngologyHead and Neck SurgeryWest China HospitalSichuan UniversityChengduSichuan610041PR China Department of Medical GeneticsWest China Second HospitalSichuan UniversityChengduSichuan610041PR China
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2023年第24卷第6期
页 面：54-68页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）]
基 金：sponsored by the National Natural Science Foundation of China(Grant No.32171351 and 82000969) the“1.3.5”Project for Disciplines of Excellence,West China Hospital,Sichuan University(Grant No.ZYJC18002) Post-Doctor Research Project,West China Hospital,Sichuan University(Grant No.2021HXBH005) Key Research and Development Program of Sichuan Province(Grant No.2021YFS0216)。
主 题：Glottic insufficiency Self-fusion Hydrogel Extracellular matrix Physiological function
摘 要：Injection laryngoplasty with biomaterials is an effective technique to treat glottic insufficiency.However,the inadequate durability,deficient pro-secretion of extracellular matrix(ECM)and poor functional preservation of current biomaterials have yielded an unsatisfactory therapeutic effect.Herein,a self-fusing bioactive hydrogel comprising modified carboxymethyl chitosan and sodium alginate is developed through a dual-crosslinking mechanism(photo-triggered and dynamic covalent bonds).Owing to its characteristic networks,the synergistic effect of the hydrogel for vocal folds(VFs)vibration and phonation is adequately demonstrated.Notably,owing to its inherent bioactivity of polysaccharides,the hydrogel could significantly enhance the secretion of major components(type I/III collagen and elastin)in the lamina propria of the VFs both in vivo and in vitro.In a rabbit model for glottic insufficiency,the optimized hydrogel(C1A1)has demonstrated a durability far superior to that of the commercially made hyaluronic acid(HA)Gel.More importantly,owing to the ECM-inducing bioactivity,the physiological functions of the VFs treated with the C1A1 hydrogel also outperformed that of the HA Gel,and were similar to those of the normal VFs.Taken together,through a simple-yet-effective strategy,the novel hydrogel has demonstrated outstanding durability,ECM-inducing bioactivity and physiological function preservation,therefore has an appealing clinical value for treating glottic insufficiency.
维普期刊数据库