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2015—2022年江苏省柯萨奇病毒A组4型全基因组序列特征分析

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中华微生物学和免疫学杂志 2024年第3期44卷 249-258页

作者：樊欢鲍倡俊朱立国胡建利嵇红江苏省疾病预防控制中心急性传染病防制所卫生部肠道病原微生物重点实验室南京210009

目的回顾性分析2015—2022年江苏省柯萨奇病毒A组4型(CVA4)的分子流行病学特征及重组情况。方法收集疱疹性咽峡炎和手足口病患者咽拭子或肛拭子标本,采用荧光定量PCR检测试剂盒对CVA4进行鉴定。利用72个CVA4完整基因组序列、99个CVA4 VP... 详细信息

目的回顾性分析2015—2022年江苏省柯萨奇病毒A组4型(CVA4)的分子流行病学特征及重组情况。方法收集疱疹性咽峡炎和手足口病患者咽拭子或肛拭子标本,采用荧光定量PCR检测试剂盒对CVA4进行鉴定。利用72个CVA4完整基因组序列、99个CVA4 VP1序列进行全面系统的系统发育分析,同时采用生物信息学软件DNAStar、MEGA7.0和Similarity plots3.5.1对江苏省CVA4分离株同源性、基因重组和氨基酸变异位点进行分析。结果根据现有VP1核苷酸序列CVA4被分为A、B、C和D基因型,基因型C可以进一步分为C1~C55个亚基因型,其中,C2基因型是与手足口病相关的CVA4主要亚基因型。江苏省分离株序列在P1区与CVA4原型具有较高的同源性,而在P2和P3区与其他肠道病毒A组(EV-A)流行株具有较高的同源性。重组分析表明,江苏省分离株在P2、P3和3′-UTR区与其他EV-A流行株存在3种基因重组模式,这些重组模式是CVA4在人群中持续和广泛传播过程中获得的,更利于CVA4传播。结论分析28个江苏省分离株CVA4完整基因组的系统发育和分子特征,丰富了已知CVA4基因组多样性;研究基因重组和VP1氨基酸位点突变情况、及时把控CVA4感染及疾病发生造成的影响,可更好地了解CVA4的进化关系和传播途径,从而帮助指导疾病控制和预防。

关键词：手足口病柯萨奇病毒A4 分子特征系统发育基因重组

2010-2022年江苏省手足口病暴发疫情流行病学特征及其影响因素分析

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中国公共卫生 2024年第3期40卷 336-341页

作者：李明阳程晓庆魏叶樊欢张雪峰鲍倡俊尚庆香嵇红南通市疾病预防控制中心急性传染病防制科南通226000 江苏省现场流行病学培训项目南京210000 江苏省疾病预防控制中心急性传染病防制所南京210000 南京医科大学公共卫生学院南京210000

目的了解江苏省2010-2022年手足口病(HFMD)暴发疫情流行病学特征及其影响因素,为当地HFMD的预防控制提供参考依据。方法收集中国疾病预防控制中心突发公共卫生事件信息管理系统中江苏省2010年1月1日-2022年12月31日上报的238起HFMD暴... 详细信息

目的了解江苏省2010-2022年手足口病(HFMD)暴发疫情流行病学特征及其影响因素,为当地HFMD的预防控制提供参考依据。方法收集中国疾病预防控制中心突发公共卫生事件信息管理系统中江苏省2010年1月1日-2022年12月31日上报的238起HFMD暴发疫情的相关数据,对江苏省HFMD暴发疫情的流行病学特征进行统计学描述,应用ArcGIS 10.7和GeoDa1.18软件分析其空间分布特征,并采用多因素非条件logistic回归模型分析HFMD暴发疫情罹患率和持续时间的主要影响因素。结果江苏省2010-2022年上报的238起HFMD暴发疫情累计暴露人数167 934人,累计发病数5 296例,暴发疫情罹患率中位数为3.43%;HFMD暴发疫情在春季(3-5月)和秋季(9-11月)高发,分别占36.97%和29.83%;有68.07%的HFMD暴发疫情由苏南地区报告,暴发疫情发生的主要场所为托幼机构或学校,占99.16%;HFMD暴发疫情发现时间和持续时间分别为0~44 d和3~78 d,发现时间和持续时间中位数分别为8 d和15.5 d;HFMD暴发疫情流行优势毒株在2010、2012、2014、2015、2018、2019和2021年均以柯萨奇病毒A16型(Cox A16)为主,2011、2013和2017年均以肠道病毒71型(EV71)为主,2020年以柯萨奇病毒A6型(Cox A6)为主,2016年以Cox A16和EV71为主,2022年以Cox A16和Cox A6为主;HFMD暴发疫情在空间上呈现聚集性分布,暴发疫情罹患率的高-高聚集区主要位于无锡市惠山区、滨湖区、梁溪区、江阴市,常州市武进区、溧阳市和连云港市赣榆区、海州区、连云区;多因素非条件logistic回归分析结果显示,Cox A16感染和混合感染、疫情持续时间≥21 d、报告来源为其他机构的江苏省HFMD暴发疫情罹患率较高,暴发场所为其他机构的江苏省HFMD暴发疫情罹患率较低,事件级别为一般、场所地为县镇、疫情发现时间≥15 d的江苏省HFMD暴发疫情持续时间较长。结论江苏省2010-2022年HFMD暴发疫情主要发生在春、秋两季,Cox A16为主要流行株,苏南地区为HFMD暴发的聚集区;暴发场所、感染病毒类型、疫情持续时间、报告来源和事件级别、场所地、疫情发现时间分别为江苏省HFMD暴发疫情罹患率和持续时间的主要影响因素。

关键词：手足口病(HFMD) 暴发疫情流行病学特征空间分布特征影响因素

G Ⅱ.17[P17]型诺如病毒基因系统发育聚类特征分析

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国际病毒学杂志 2024年第1期31卷 8-11页

作者：李楚楚艾静秦圆方朱立国胡建利鲍倡俊付建光江苏省疾病预防控制中心卫健委肠道病原微生物重点实验室南京210009

目的了解GⅡ.17[P17]型诺如病毒不同基因的发育聚类特征,为GⅡ.17[P17]序列的准确聚类提供依据.方法收集2023年3月至5月聚集性疫情中的GⅡ.17[P17]阳性标本,二代测序获得全基因组序列,与GenBank下载的参考株序列共同构建进化树,分别对... 详细信息

目的了解GⅡ.17[P17]型诺如病毒不同基因的发育聚类特征,为GⅡ.17[P17]序列的准确聚类提供依据.方法收集2023年3月至5月聚集性疫情中的GⅡ.17[P17]阳性标本,二代测序获得全基因组序列,与GenBank下载的参考株序列共同构建进化树,分别对各基因(Nterm、NTP、P22、VPg、Pro、RdRp、VP1和VP2)序列进行系统发育聚类分析及熵值分析.结果16个GⅡ.17[P17]阳性标本中共14个获得了全基因组序列,测序深度及覆盖度均较高.首先构建RdRp及VP1基因的系统发育进化树,发育聚类均为6个簇(ClusterⅠ~Ⅵ),其中ClusterⅠ~Ⅳ聚类相同,Cluster V~Ⅵ聚类有少量差异.2023年的标本株序列位于Cluster V.以RdRp聚类为基准,对病毒的8个基因片段进行聚类分析,发现NTP、RdRp和VP1基因聚类效果较好,6个簇均未形成新的分支.此外,这三个基因的熵值位点构成比也处于较低水平.结论诺如病毒GⅡ.17[P17]型别不同基因的系统发育聚类有一定差异,其中NTP、RdRp和VP1基因聚类效果较好.

关键词：诺如病毒基因系统发育聚类

Effects of closing and reopening live poultry markets on the epidemic of human infection with avian influenza A virus

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The Journal of Biomedical Research 2016年第2期30卷 112-119页

作者： Jian Lu Wendong Liu Rui Xia Qigang Dai changjun bao Fenyang Tang Yefei Zhu Qiao Wang School of Information Science and Engineering Southeast University Department of Acute Infectious Disease Control and Prevention Jiangsu Provincial Center for Disease Control and Prevention Center of Laboratory Medicine The Second Affiliated Hospital of Nanjing Medical University

Live poultry markets（LPMs） are crucial places for human infection of influenza A（H7N9 virus）.In Yangtze River Delta,LPMs were closed after the outbreak of human infection with avian influenza A（H7N9） virus,and then reopened when no case was *** purpose was to quantify the effect of LPMs＇ operations in this region on the transmission of influenza A（H7N9） *** obtained information about dates of symptom onset and locations for all human influenza A（H7N9） cases reported from Shanghai,Jiangsu and Zhejiang provinces by May 31,2014,and acquired dates of closures and reopening of LPMs from official media.A two-phase Bayesian model was fitted by Markov Chain Monte Carlo methods to process the spatial and temporal influence of human cases.A total of 235 cases of influenza A（H7N9） were confirmed in Shanghai,Jiangsu and Zhejiang by May 31,*** these data,our analysis showed that,after LPM closures,the influenza A（H7N9） outbreak disappeared within two weeks in Shanghai,one week in Jiangsu,and one week in Zhejiang,*** authorities reopened LPMs when there was no outbreak of influenza A（H7N9）,which did not lead to reemergence of human influenza A（H7N9）.LPM closures were effective in controlling the H7N9 *** of LPM in summer did not increase the risk of human infection with *** findings showed that LPMs should be closed immediately in areas where the H7N9 virus is confirmed in *** there is no outbreak of H7N9 virus,LPMs can be reopened to satisfy the Chinese traditional culture of buying live *** the long term,local authorities should take a cautious attitude in permanent LPM closure.

关键词： live poultry market avian influenza A(H7N9)

A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus

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Journal of Integrative Agriculture 2024年第5期23卷 1658-1670页

作者： Lingzhai Meng Mengmeng Yu Suyan Wang Yuntong Chen Yuanling bao Peng Liu Xiaoyan Feng Tana He Ru Guo Tao Zhang Mingxue Hu changjun Liu Xiaole Qi Kai Li Li Gao Yanping Zhang Hongyu Cui Yulong Gao Avian Immunosuppressive Diseases Division State Key Laboratory for Animal Disease Control and PreventionHarbin Veterinary Research InstituteChineseAcademy of Agricultural SciencesHarbin150069China Avian ImmunosuppressiveDiseases DivisionStateKey LaboratoryforAnimal Disease Cont National Poultry Laboratory Animal Resource Center Harbin 150069China

Avian metapneumovirus(aMPV) is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys, resulting in serious economic losses. Subtype B aMPV has recently become the dominant epidemic strain in China. We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate. The safety test showed that after the 30th passage, the LN16-A strain was fully attenuated, as clinical signs of infection and histological lesions were absent after *** LN16-A strain did not revert to a virulent strain after five serial passages in chickens. The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16) strain and had nine amino acid mutations. In chickens, a single immunization with LN16-A induced robust humoral and cellular immune responses, including the abundant production of neutralizing antibodies, CD4^(+) T lymphocytes, and the Th1(IFN-γ) and Th2(IL-4 and IL-6)cytokines. We also confirmed that LN16-A provided 100% protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation. Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.

关键词： avian metapneumovirus attenuated protection vaccine candidate chickens

Weighted Markov chains for forecasting and analysis in Incidence of infectious diseases in jiangsu Province,China

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The Journal of Biomedical Research 2010年第3期24卷 207-214页

作者： Zhihang Peng changjun bao Yang Zhao Honggang Yi Letian Xia Hao Yu Hongbing Shen Feng Chen Department of Epidemiology and Biostatistics Nanjing Medical University School of Public Health Nanjing 210029 Jiangsu Province China Center for Disease Control and Prevention of Jiangsu Province Nanjing 210029 Jiangsu Province China Applied Mathematics Department Hohai University Nanjing 210029 Jiangsu Provinee China

This paper first applies the sequential cluster method to set up the classification standard of infectious disease incidence state based on the fact that there are many uncertainty characteristics in the incidence *** the paper presents a weighted Markov chain,a method which is used to predict the future incidence *** method assumes the standardized self-coefficients as weights based on the special characteristics of infectious disease incidence being a dependent stochastic *** also analyzes the characteristics of infectious diseases incidence via the Markov chain Monte Carlo method to make the long-term benefit of decision *** method is successfully validated using existing incidents data of infectious diseases in Jiangsu *** summation,this paper proposes ways to improve the accuracy of the weighted Markov chain,specifically in the field of infection epidemiology.

关键词： weighted.Markov chains sequential cluster infectious diseases forecasting and analysis Markov chain Monte Carlo

2015—2022年江苏省柯萨奇病毒A10型全基因组序列特征分析

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中华微生物学和免疫学杂志 2023年第12期43卷 945-954页

作者：樊欢鲍倡俊朱立国胡建利嵇红江苏省疾病预防控制中心急性传染病防制所卫生部肠道病原微生物重点实验室南京210009

目的对江苏省2015—2022年柯萨奇病毒A10型(CVA10)分离株全基因组序列特征进行分析,阐明其分子流行病学特征。方法选取2015—2022年间江苏省地区流行的45株CVA10分离株进行全基因组测序,基于CVA10全基因组、VP1、P1、P2和P3区序列分别... 详细信息

目的对江苏省2015—2022年柯萨奇病毒A10型(CVA10)分离株全基因组序列特征进行分析,阐明其分子流行病学特征。方法选取2015—2022年间江苏省地区流行的45株CVA10分离株进行全基因组测序,基于CVA10全基因组、VP1、P1、P2和P3区序列分别构建系统进化树,运用DNAStar、MEGA7.0和Similarity plots3.5.1等生物信息学软件对获取的全基因组序列进行比对,分析同源性、基因重组情况和主要氨基酸变异位点。结果45株CVA10分离株全基因组核苷酸和氨基酸序列同源性分别为90.3%~99.1%和97.9%~99.8%;各区段比对发现,P1区核苷酸序列同源性最高,为92.1%~100.0%;P3区段序列同源性差异较大为84.7%~100.0%,但相比于核苷酸序列的多样性,各区段氨基酸序列均较为保守。基于VP1序列的系统进化树将CVA10分为A~H共8个基因型,其中江苏与其他国内大部分流行株均属于C基因型,全基因组进化树和VP1进化树分析结果接近一致。基于基因组各区段序列的系统进化与Simplot重组分析结果显示,江苏分离株GD07/Lianyungang/2017和N180/Suqian/2016与CVA10原型株在P1区域高度匹配,而在非结构蛋白(P2、P3)、5′-UTR和3′-UTR区均观察到与其他EVA存在重组信号。而氨基酸突变位点分析结果显示,与原型株AY421767/Kowalik/2004相比,VP1区以及多个重要氨基酸位点变异频繁,这可能会使得CVA10衣壳蛋白的结构、潜在的受体结合位点产生一些难以察觉的变化。结论本研究通过探究江苏地区CVA10在基因组水平的进化方向和重组特征,及时把控基因重组和氨基酸位点突变对CVA10感染及疾病发生造成的影响,为江苏省手足口病的防控工作提供了基础资料。

关键词：柯萨奇病毒A10 手足口病全基因组分子特征系统发育基因重组

Risk assessment on the epidemics of human infection with a novel avian influenza A (H7N9) virus in Jiangsu Province, China

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The Journal of Biomedical Research 2013年第3期27卷 163-166页

作者： Wendong Liu Yefei Zhu Xian Qi Ke Xu Aihua Ge Hong Ji Jing Ai changjun bao Fenyang Tang Minghao Zhou Department of Acute Infectious Disease Control and Prevention Jiangsu Provincial Center for Disease Control and Prevention

A novel avian influenza A （H7N9） virus was discovered in February 2013 in China and has resulted in more than 100 comfirmed human infections including 26 fatal cases as of May 2, 2013. The situation raises many ur- gent questions and global public health concerns. In this study, epidemiologic characteristics of infected human cases in Jiangsu province were analyzed and risk assessment was undertaken based on the information available. Briefly, it is highly unlikely that a pandemic of human infection with avian influenza A （HTN9） virus will happen in Jiangsu Province in the near future. Iia the end, some measures are recommended to prevent the situation from becoming worse.

关键词： avian influenza H7N9 risk assessment

Surveillance on the Immune Effectiveness of Quadrivalent and Trivalent Split Influenza Vaccines—Shenzhen City and Changzhou City,China,2018–2019

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China CDC weekly 2020年第21期2卷 370-375页

作者： Liumei Shu Jie Zhang Xiang Huo Cong Chen Shisong Fang Kexin Zong Yuanyuan Guo Yingze Zhao Jun Zhang changjun bao Junjie Xia Fengcai Zhu Dayan Wang Guizhen Wu George F.Gao William J.Liu NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Jiangsu Provincial Center for Disease Control and Prevention NanjingChina Changzhou Center for Disease Control and Prevention ChangzhouChina Division of Microbiology Test Shenzhen Centre for Disease Control and PreventionShenzhenChina School of Laboratory Medicine and Life Sciences Wenzhou Medical UniversityWenzhouChina School of Pharmaceutical Sciences Nanjing Tech UniversityNanjingChina CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina.

Summary What is already known about this topic?Vaccinations are the most effective way to prevent influenza virus infections and severe *** vaccine effectiveness can vary by *** is added by this report?This report monitors the antibody level among the population over time after administration of the quadrivalent or trivalent split influenza *** are the implications for public health practice?Real-time monitoring of serum antibody changes after vaccination provides important data for the development of reasonable and effective strategies for influenza prevention and control.

关键词： ent prevention valent