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Mediating Mesenchymal Stem Cells Responses and Osteopontin Adsorption via Oligo(ethylene glycol)-amino Mixed Self-assembled Monolayers

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Journal of Materials Science & Technology 2016年第9期32卷 966-970页

作者： Lijing Hao Tianjie Li Naru Zhao fuzhai cui Chang Du Yingjun Wang School of Materials Science and Engineering South China University of TechnologyGuangzhou 510640China National Engineering Research Center for Tissue Restoration and Reconstruction Guangzhou 510006China Department of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

Oligo（ethylene glycol）（-OEG） and amino （-NH2） mixed self-assembled monolayers （SAMs） were employed as model substrates to investigate the effect of charge density on the fate of mesenchymal stem cells （MSCs） and osteopontin （OPN） adsorption. We found that all surfaces presenting -NH2 groups favored cell responses regardless of the surface charge. Meanwhile, OPN adsorption could remain stable on the mixed SAMs over a certain range of charge densities. Our work provides some insights into cell responses and protein adsorption to surface charge.

关键词： Mixed self-assembled monolayers Oligo(ethylene glycol)-amino Mesenchymal stem cell Osteopontin

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Preparation of 3-D porous fibroin scaffolds by freeze drying with treatment of methanol solutions

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Chinese Science Bulletin 2007年第13期52卷 1791-1795页

作者： ZHAN JingLin SUN XiaoDan cui fuzhai KONG XiangDong Laboratory of Advanced Materials Department of Materials Science & Engineering Tsinghua University Beijing 100084 China Xinyuan Institute of Medicine and Biotechnology College of Life Science Zhejiang Science and Technology University Hangzhou 310029 China

In this study, silk scaffolds with appropriate porous structures were prepared by adjusting solution concentrations and providing treatment with methanol solutions in the way of freeze drying. The effects of the preparation conditions on the microstructures and properties of the scaffolds were discussed. Fibroin solutions with different concentrations of 4, 6, 8, 10 wt% were used respectively to prepare the scaffolds. The effects of the addition of 20 vol% methanol before or after freeze drying to the 4 wt% fibroin solution were investigated. As demonstrated by Scanning Electron Microscope (SEM), the fib-roin scaffolds prepared without methanol had porous microstructures composed of thin sheets, and the sizes of the pores decreased with the increase of the fibroin solution concentrations, while the scaffolds prepared in the presence of methanol showed porous microstructures formed by fine-particle aggregates. The porosities and mechanical properties of the prepared fibroin scaffolds under different conditions were tested. The crystalline structures and conformations of the fibroin scaffolds were detected by Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD).

关键词：蚕丝蛋白多孔材料冷藏干燥甲醇

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Directing neural stem cell fate with biomaterial parameters for injured brain regeneration

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Progress in Natural Science:Materials International 2013年第2期23卷 103-112页

作者： Shenglian Yao Xi Liu Xiumei Wang Antonio Merolli Xiongbiao Chen fuzhai cui Institute for Regenerative Medicine and Biomimetic Materials Key Laboratory of Advanced Materials School of Materials Science and Engineering Tsinghua University National Engineering Laboratory for Regenerative Implantable Medical Devices Orthopaedics & Hand Surgery Unit The Catholic University School of Medicine Division of Biomedical Engineering College of Engineering University of Saskatchewan

The discovery of neural stem cells (NSCs), which have the ability to self-renew and differentiate into all types of neural lineages, offers promising prospect for the treatment of brain neurological disorders such as stroke/cerebral ischemia, traumatic brain injury and neurodegenerative disorders. However, only limited number of NSCs could survive or propagate due to tissue inflammation or blood-brain barrier. Therefore, it is necessary to develop an appropriate culture system that highly mimics the natural NSCs niche to direct stem cell fate and behavior for nerve regeneration. Both biophysical and biochemical properties of the NSC niche, including topology, mechanical properties, bioactive molecules, and their spatial and temporal presentations should be considered for the design of functionalized scaffolds, which could not only serve as the delivery vehicles of NSCs but also stimulate specific cellular responses at the molecular level, such as support endogenous or exogenous cells proliferation, migration and homing, even promote the growth of axon at the injured brain site. This review attempts to outline the varieties of biomaterial

关键词： NSCs niche Biomaterial design Brain tissue engineering Nerve regeneration

Biomechanical evaluation of different hydroxyapatite coatings on titanium for keratoprosthesis

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Frontiers of Materials Science 2015年第3期9卷 303-310页

作者： Ying DONG Zhiye QIU Xiaoyu LIU Liqiang WANG Jingxin YANG Yifei HUANG fuzhai cui Department of Ophthalmology The First Affiliated Hospital Chinese PLA General Hospital Beijing 100048 China Department of Ophthalmology Chinese PLA General Hospital Beijing 100853 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical Engineering Beihang University Beijing 100191 China

Stable tissue integration is important to keratoprosthesis （KPro）. The aim of this study was to evaluate the tissue bonding ability of hydroxyapatite （HAp）-coated titanium KPro. The samples were divided into three groups： test groups （IBAD group and AD group） and Ti control. The coated samples had a HAp layer created by ion beam assisted deposition （IBAD） or aerosol deposition （AD）. The surface characteristics were analyzed with SEM, AFM, and XRD. The samples were surgically inserted into the muscles of rabbits. Eight weeks after healing, the attachment to the tissue was tested with a universal test device. The three samples exhibited distinctive surface morphology. The force to remove the HAp implants from the muscles was significantly greater than that of Ti group （P〈0.01）, with the AD samples requiring the greatest force （P〈0.01）. After removal, SEM showed that the tissue was firmly attached to the surface of AD samples. Photomicrographs of the peri-implant muscles showed a layer of aligned fibrous tissue without severe inflammation. The AD samples had more fibroblasts. Results indicate that because of enhanced mechanical adhesion of soft tissue to the implants, HAp-coated Ti by AD is a suitable KPro skirt material.

关键词： hydroxyapatite (HAp) titanium keratoprosthesis (KPro) biomechanics tissue attachment

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Enhanced angiogenesis by the hyaluronic acid hydrogels immobilized with a VEGF mimetic peptide in a traumatic brain injury model in rats

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Regenerative Biomaterials 2019年第6期6卷 325-334页

作者： Jiaju Lu Fengyi Guan fuzhai cui Xiaodan Sun Lingyun Zhao Ying Wang Xiumei Wang State Key Laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced Materials of Ministry of EducationSchool of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Beijing Center of Neutral Regeneration and Repair Key Laboratory for Neurodegenerative Disease of the Ministry of EducationCapital Medical UniversityBeijing 100069China

Angiogenesis plays an important role in brain injury repair,which contributes to the reconstruction of regenerative neurovascular niche for promoting axonal regeneration in the lesion *** a major component of developing brain extracellular matrix,hyaluronic acid(HA)has attracted more attention as a supporting matrix for brain *** the present study,HA-KLT hydrogel was developed via modifying HA with a VEGF mimetic peptide of KLT(KLTWQELYQLKYKGI).The characterization of the hydrogel shows that it could provide a porous,three-dimensional scaffold structure,which has a large specific surface area available for cell adhesion and *** with the unmodified HA hydrogel,the HA-KLT hydrogel could effectively promote the attachment,spreading and proliferation of endothelial cells in ***,the pro-angiogenic ability of hydrogels in vivo was evaluated by implanting them into the lesion cavities in the injured rat *** results showed that the hydrogels could form a permissive interface with the host tissues at 4 weeks after ***,they could efficiently inhibit the formation of glial scars at the injured *** HA-KLT hydrogel could significantly increase the expression of endoglin/CD105 and promote the formation of blood vessels,suggesting that HA-KLT hydrogel promoted angiogenesis in ***,the HA-KLT hydrogel has the potential to repair brain defects by promoting angiogenesis and inhibiting the formation of glial-derived scar tissue.

关键词： traumatic brain injury hyaluronic acid angiogenesis VEGF mimetic peptide

Histopathological and imageological studies on clinical outcomes of mineralized collagen reconstruction rod for femoral head necrosis with one case report

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Regenerative Biomaterials 2017年第4期4卷 243-249页

作者： Baogang Xie Hao Wang Jianhua Hao Ping Wang Na Zhang Jingjing Wu Zhiye Qiu fuzhai cui Department of Orthopaedics 254th Hospital of PLANo.60 Huangwei RoadHebei DistrictTianjin 300142China School of Materials Science and Engineering Tsinghua UniversityHaidian District Beijing 100084China

In this article,the biodegradation process and bone formation of a mineralized collagen reconstruction rod embedding in necrosis of human femoral head were investigated by imageological and histological *** radiography(CR)computerized tomography(CT),common pathological section and hard tissue section analysis were used to evaluated the dynamics of imageological and histopathological changes of femoral head,interface between the host bone and implant and the bone reconstruction *** results showed that the density of rods increased closed to that of host bones after 1 year implanting,and the interface between them turns to *** tissue grinding sections analysis showed osteocytes appearing in sparse bone trabecular and bone pit region,as well as a few vessels in the degraded dye powder matrix were noticed,indicating the new bone forming between the implants and host *** decalcified sections analysis showed scattered osteoclasts,multinucleated giant cells and fibrosis components existing in the degraded rod and the host bone *** debris was endocytosed by giant cells,and vascular network formed around the boundaries of the implanted *** good osteointegration has been expressed by the interface between the implanted rod and the host bone becoming *** results indicated that the implanted rod degradation process and new bones regeneration simultaneously occurred around the boundaries of embedding *** bone and host bone were hinged and co-existed.

关键词： femoral head necrosis mineralized collagen reconstruction rod for femoral head necrosis

A SIMULATION STUDY OF LOW ENERGY SPUTTERING ANGULAR DISTRIBUTION

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Chinese Physics Letters 1988年第4期5卷 145-148页

作者：郑里平崔福斋 Institute of Nuclear Research Academia SinicaShanghai Ion-Beam Laboratory Institute of MetallurgyAcademia SinicaShanghai Department of Engineering Physics Qinghua UniversityBeijing

The low energy sputtering angular distributions are simulated in inert gas ion-metal target *** is found that the angular distribution of PKAs trends to an under-cosine shape,while the angular distribution of SKAs shows either a cosine one or an over-cosine one:the formation of the under-cosine sputtering angular distribution seems to come from the smaller mass ratio and the lower incident energy.

关键词： energy. angular distribution

A high-strength mineralized collagen bone scaffold for large-sized cranial bone defect repair in sheep

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Regenerative Biomaterials 2018年第5期5卷 283-292页

作者： Shuo Wang Zhijun Zhao Yongdong Yang Antonios G.Mikos Zhiye Qiu Tianxi Song fuzhai cui XiumeiWang Chunyang Zhang State Key Laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced Materials of Ministry of EducationSchool of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Department of Neurosurgery The First Affiliated Hospital of Baotou Medical SchoolBaotou 014010China Dongzhimen Hospital Affiliated Beijing University of Chinese Medicine Beijing 100700China Department of Bioengineering Rice UniversityHoustonTX 77030USA Beijing Allgens Medical Science and Technology Co. Ltd.Beijing 100176China

Large-sized cranial bone defect repair presents a great challenge in the *** ideal cranioplasty materials to realize the functional and cosmetic recovery of the defect must have sufficient mechanical support,excellent biocompatibility,good osseointegration and biodegradability as *** this study,a high-strength mineralized collagen(MC)bone scaffold was developed with biomimetic composition,microstructure and mechanical properties for the repair of sheep largesized cranial bone defects in comparison with two traditional cranioplasty materials,polymethyl methacrylate and titanium *** compact MC scaffold showed no distinct pore structure and therefore possessed good mechanical *** strength and elastic modulus of the scaffold were much higher than those of natural cancellous bone and slightly lower than those of natural compact *** vitro cytocompatibility evaluation revealed that the human bone marrow mesenchymal stem cells(hBMSC)had good viability,attachment and proliferation on the compact MC scaffold indicating its excellent *** adult sheep cranial bone defect model was constructed to evaluate the performances of these cranioplasty materials in repairing the cranial bone *** results were investigated by gross observation,computed tomography scanning as well as histological *** in vivo evaluations indicated that compact MC scaffold showed notable osteoconductivity and osseointegration with surrounding cranial bone tissues by promoting bone *** results suggested that the compact MC scaffold has a promising potential for large-sized cranial bone defect repair.

关键词： mineralized collagen cranial bone defect bone regeneration sheep cranioplasty materials

Gene expression profiling and mechanism study of neural stem cells response to surface chemistry

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Regenerative Biomaterials 2014年第1期1卷 37-47页

作者： Ying Wang Shenglian Yao Qingyuan Meng Xiaolong Yu Xiumei Wang fuzhai cui Institute for regenerative medicine and biomimetic materials School of materials science and engineeringTsinghua UniversityBeijing 100084China Department of anatomy histology and embryologySchool of basic medical sciencesCapital Medical UniversityBeijing 100069China Department of material science and chemical engineering Hainan UniversityHaikou 570228China

To declare the mechanisms of neural stem cells(NSCs)in response to material surface chemistry,NSCs were exposed to the self-assemble monolayers of alkanethiolates on gold surfaces terminated with amine(NH2),hydroxyl(OH)and methyl(CH3)for *** morphological responses of NSCs were recorded;the gene expression profilings were detected by genechips;the gene expressions data of NSCs responded to different chemical groups were declared through the gene ontology term and pathway *** showed that cells behaved dissimilar on the three chemical groups,the adhesion,proliferation and migration were easier on the NH2 and OH groups;the gene expressions of NSCs were induced differently,either,involved in several functional processes and signaling ***3 group induced genes enriched much in chemistry reactions and death processes,whereas many genes of cellular nucleotide metabolism were ***2 group induced NSCs to express many genes of receptors on membrane,and participated in cellular signal transduction of cell adhesion and interactions,or associated with axon *** group was similar to NH2 group to induce the membrane response,but it also down regulated metabolism of ***,it declared the chemical groups affected NSCs through inner way and the NH2,OH and CH3 groups triggered the cellular gene expression in different signaling pathways.

关键词： neural stem cell chemical group biomaterial gene expression receptor signaling pathway