Characterization of astrocytes and microglial cells in the hippocampal CA1 region after transient focal cerebral ischemia in rats treated with Ilexonin A

作     者：Ai-Ling Xu Guan-Yi Zheng Hui-Ying Ye Xiao-Dong Chen Qiong Jiang Ai-Ling Xu;Guan-Yi Zheng;Hui-Ying Ye;Xiao-Dong Chen;Qiong Jiang

作者机构：Department of Traditional Chinese MedicineFujian Medical University Union HospitalFuzhouFujian ProvinceChina Department of NeonatologyPeople’s Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhouFujian ProvinceChina Department of NeurologyPeople’s Hospital of NanpingNanpingFujian ProvinceChina Burns Institute of Fujian Medical University Union HospitalFuzhouFujian ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第1期

页      面：78-85页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the Natural Science Foundation of Fujian Province of China,No.2014J01327 the Program for New Century Excellent Talents in Colleges and Universities of Fujian Province of China,No.NCETFJ-0704 the Professorial Academic Development Foundation of Fujian Medical University of China,No.JS09014(all to GYZ) 

主　　题：astrocytes hippocampal CA1 region ilexonin A microglia middle cerebral artery occlusion neural stem cell neuroprotection transient focal cerebral ischemia 

摘      要：Ilexonin A is a compound isolated from the root of Ilex pubescens,a traditional Chinese medicine.Ilexonin A has been shown to play a neuroprotective role by regulating the activation of astrocytes and microglia in the peri-infarct area after ischemia.However,the effects of ilexonin A on astrocytes and microglia in the infarct-free region of the hippocampal CA1 region remain unclear.Focal cerebral ischemia models were established by 2-hour occlusion of the middle cerebral artery in rats.Ilexonin A(20,40 or 80 mg/kg)was administered immediately after ischemia/reperfusion.The astrocyte marker glial fibrillary acidic protein,microglia marker Iba-1,neural stem cell marker nestin and inflammation markers were detected by immunohistochemistry and western blot assay.Expression levels of tumor necrosis factor-αand interleukin 1βwere determined by enzyme linked immunosorbent assay in the hippocampal CA1 tissue.Astrocytes were activated immediately in progressively increasing numbers from 1,3,to 7 days post-ischemia/reperfusion.The number of activated astrocytes further increased in the hippocampal CA1 region after treatment with ilexonin A.Microglial cells remained quiescent after ischemia/reperfusion,but became activated after treatment with ilexonin A.Ilexonin A enhanced nestin expression and reduced the expression of tumor necrosis factor-αand interleukin 1βin the hippocampus post-ischemia/reperfusion.The results of the present study suggest that ilexonin A has a neuroprotective effect in the hippocampus after ischemia/reperfusion,probably through regulating astrocytes and microglia activation,promoting neuronal stem cell proliferation and reducing the levels of pro-inflammatory factors.This study was approved by the Animal Ethics Committee of the Fujian Medical University Union Hospital,China.