Prevalence of HIV Indeterminate Western Blot Tests and Follow-up of HIV Antibody Sero-Conversion in Southeastern China

作     者：Shouli Wu Min Gao Jian Zheng Pingping Yan Wei Wang Xiaoli Lu Yuefeng Qiu Yansheng Yan 

作者机构：Fujian Provincial Center for Disease Control and PreventionFuzhou 350001China Fujian Provincial Key Laboratory of Zoonosis ResearchFuzhou 350001China School of Public HealthFujian Medical UniversityFuzhou 350004China Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhou 350025China Key Laboratory of National Health Commission on Parasitic Disease Control and PreventionWuxi 214064China Jiangsu Provincial Key Laboratory on Parasite and Vector Control TechnologyWuxi 214064China Jiangsu Institute of Parasitic DiseasesWuxi 214064China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2019年第34卷第4期

页      面：358-366页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 

基　　金：supported by grants from the Cultivation of Young Talents Project Fund from the Fujian Provincial Health and Family Planning Commission (Grant No. 2015-ZQN-ZD11) the Pilot Project of Fujian Provincial Department of Science and Technology (Grant No. 2016Y0010) the Jiangsu Provincial Project of Invigorating Health Care through Science, Technology and Education Jiangsu Provincial Medical Youth Talent the Project of Invigorating Health Care through Science, Technology and Education (Grant No. QNRC2016621) 

主　　题：Human immunodeficiency virus(HIV) HIV indeterminate result Western blotting Nucleic acid test Differential diagnosis 

摘      要：HIV-indeterminate Western blotting(WB)results are typically obtained in WB confirmatory assays,and the number of indeterminate samples may increase with the detection of HIV infections,which will present considerable challenges for the management of HIV/*** acid detection has been used as a laboratory test for screening suspected or indeterminate ***,the effectiveness of these assays for the differential diagnosis of HIV-indeterminate WB samples remained *** this study,210 subjects with HIV-indeterminate WB results were detected from 6360 positive HIV screening samples between 2015 and 2016 in southeastern China,in which HIV-indeterminate WB results accounted for 3.30%.The highest proportion of indeterminate results was observed in pregnant and lying-in women receiving physical examinations(16.67%),followed by that in voluntary blood donors(8.82%).The most common WB band patterns were p24,gpl60 and p24,and *** follow-up study revealed that the highest negative and positive conversion rates of HIV antibodies were in samples with a single p24 band(80.28%),and with gpl60 and p24 bands(86.21%),*** the Env,Gag,and Pol antibodies,samples with a Gag band showed the highest negative conversion rate(81.25%),whereas the highest positive conversion rate was observed in samples with an Env band(56.76%).In addition,quantitative and qualitative HIV nucleic acid testing exhibited the highest sensitivity(96.3%)and specificity(97.85%),*** results indicate a lower proportion of HIV indeterminate WB results in southeastern China compared to previous reports,and the follow-up re-examination of patients with HIV indeterminate results should be *** acid testing facilitates the identification of HIV infections.