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Genotypes Diversity of Acute Gastroenteritis Outbreaks Caused by Human Sapovirus-Beijing Municipality,China,2015-2021

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China CDC weekly 2023年第28期5卷 625-631页

作者： Yang Jiao Taoli Han Xiao Qi Yan Gao Jianhong Zhao Yue Zhang Beibei Li Zheng Zhang Jialiang Du Lingli Sun Beijing Chaoyang District Center for Disease Control and Prevention BeijingChina National Institutes for Food and Drug Control BeijingChina

Introduction:Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of acute gastroenteritis(AGE)globally.A seven-year active surveillance study was conducted to investigate the molecular epidemiology of HuSaVs associated with AGE outbreaks in Chaoyang District of Beijing Municipality,China from January 2015 to December 2021.Methods:Fecal and anal swab samples were obtained from patients experiencing AGE outbreaks.HuSaVs were identified through reverse transcription polymerase chain reaction(RT-PCR),and partial viral protein 1(VP1)sequences(approximately 434 base pairs)were utilized for genotyping,single nucleotide polymorphism(SNP)analysis,and phylogenetic examination.Results:HuSaVs were identified in 71 AGE outbreaks,demonstrating a detection rate of 10.5%,second only to norovirus.The primary demographic affected by HuSaV were children under the age of 5 in kindergarten settings.Infection rates tended to peak during two distinct periods:May to June and September to December.Upon genotyping,seven distinct genotypes emerged.GII.3 was the most prevalent,accounting for 54.9%of cases,followed by GI.1(12.7%),GI.2(9.9%),GII.5(7.0%),GI.5(2.8%),GI.6(1.4%),GII.1(1.4%),and untyped cases(9.9%).A phylogenetic analysis of GII.3 identified three distinct groups,with 15 notable SNPs observed.Conclusions:This study offers a comprehensive analysis of the persistent prevalence of HuSaV outbreaks in Chaoyang District,Beijing Municipality,China.Over time,the diversity of HuSaV subtypes has shifted,and it is now recognized as the second leading viral agent responsible for AGE outbreaks.This highlights the importance of ongoing surveillance in the future.

关键词： epidemiology Human enteritis

Establishment of a Special Platform for the Research of Prion and the Diagnosis of Human Prion Disease —China’s Studies

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China CDC weekly 2022年第33期4卷 729-734页

作者： Kang Xiao Qi Shi Cao Chen Wei Zhou Chen Gao Liping Gao Jun Han Jichun Wang Xiaoping Dong State Key Laboratory for Infectious Disease Prevention and Control NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina China Academy of Chinese Medical Sciences BeijingChina Center for Biosafety Mega-Science Chinese Academy of SciencesWuhan CityHubei ProvinceChina Division of Science and Technology Chinese Center for Disease Control and PreventionBeijingChina Shanghai Institute of Infectious Disease and Biosafety Shanghai MunicipalityChina

The studies of prions and prion disease usually need many special platforms and techniques that differ from those for classical microbes.Search of new biomarkers and establishment of new methods for the diagnosis of h... 详细信息

关键词： Human diagnosis Prion

Injectable collagen scaffold with Human umbilical cordderived mesenchymal stem cells promotes functional recovery in patients with spontaneous intracerebral hemorrhage:phase Ⅰ clinical trial

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Neural Regeneration Research 2023年第9期18卷 1999-2004页

作者： Xiao-Yin Li Wu-Sheng Deng Zi-Qi Wang Zheng-Chao Li Shu-Lian Chen Zhen Song Quan Zhang Jin Liang Xu-Yi Chen Characteristic Medical Center of Chinese People’s Armed Police Force Tianjin Key Laboratory of Neurotrauma RepairTianjinChina Department of Neurosurgery Dazhou Second People’s HospitalDazhouSichuan ProvinceChina Graduate School of Tianjin University of Traditional Chinese Medicine TianjinChina

Animal expe riments have shown that injectable collagen scaffold with Human umbilical cord-derived mesenchymal stem cells can promote recovery from spinal cord injury.To investigate whether injectable collagen scaffold with Human umbilical cord-derived mesenchymal stem cells can be used to treat spontaneous intracerebral hemorrhage,this non-randomized phase I clinical trial recruited patients who met the inclusion criteria and did not meet the exclusion crite ria of spontaneous intracerebral hemorrhage treated in the Characteristic Medical Center of Chinese People’s Armed Police Force from May 2016 to December 2020.Patients were divided into three groups according to the clinical situation and patient benefit:control(n=18),Human umbilical cord-derived mesenchymal stem cells(n=4),and combination(n=8).The control group did not receive any transplantation.The Human umbilical cord-derived mesenchymal stem cells group received Human umbilical cord-derived mesenchymal stem cell transplantation.The combination group received injectable collagen scaffold with Human umbilical cord-derived mesenchymal stem cells.Patients who received injectable collagen scaffold with Human umbilical cord-derived mesenchymal stem cells had more remarkable improvements in activities of daily living and cognitive function and smaller foci of intra cerebral hemorrhage-related encephalomalacia.Severe adve rse events associated with cell transplantation were not observed.Injectable collagen scaffold with Human umbilical cord-derived mesenchymal stem cells appears to have great potential treating spontaneous intracerebral hemorrhage.

关键词： clinical trial collagen scaffold efficacy Human umbilical cord-derived mesenchymal stem cells Human safe neurological recovery spontaneous intracerebral hemorrhage transplantation

Decoding transcriptional regulation via a Human gene expression predictor

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Journal of Genetics and Genomics 2023年第5期50卷 305-317页

作者： Yuzhou Wang Yu Zhang Ning Yu Bingyan Li Jiazhen Gong Yide Mei Jianqiang Bao Shisong Ma The First Affiliated Hospital of USTC Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230027China MOE Key Laboratory for Cellular Dynamics School of Life SciencesDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaInnovation Academy for Seed DesignChinese Academy of SciencesHefeiAnhui 230027China School of Data Science University of Science and Technology of ChinaHefeiAnhui 230027China

Transcription factors(TFs)regulate cellular activities by controlling gene expression,but a predictive model describing how TFs quantitatively modulate Human transcriptomes is lacking.We construct a universal Human gene expression predictor named EXPLICIT-Human and utilize it to decode transcriptional regulation.Using the expression of 1613 TFs,the predictor reconstitutes highly accurate transcriptomes for samples derived from a wide range of tissues and conditions.The broad applicability of the predictor indicates that it recapitulates the quantitative relationships between TFs and target genes ubiquitous across tissues.Significant interacting TF-target gene pairs are extracted from the predictor and enable downstream inference of TF regulators for diverse pathways involved in development,immunity,metabolism,and stress response.A detailed analysis of the hematopoiesis process reveals an atlas of key TFs regulating the development of different hematopoietic cell lineages,and a portion of these TFs are conserved between Humans and mice.The results demonstrate that our method is capable of delineating the TFs responsible for fate determination.Compared to other existing tools,EXPLICIT-Human shows a better performance in recovering the correct TF regulators.

关键词： Gene expression predictor Gene regulatory network Graphical Gaussian model Gene module Human Mouse

Dynamic Human retinal pigment epithelium(RPE)and choroid architecture based on single-cell transcriptomic landscape analysis

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Genes & Diseases 2023年第6期10卷 2540-2556页

作者： Lulin Huang Lin Ye Runze Li Shanshan Zhang Chao Qu Shujin Li Jie Li Mu Yang Biao Wu Ran Chen Guo Huang Bo Gong Zheng Li Hongjie Yang Man Yu Yi Shi Changguan Wang Wei Chen Zhenglin Yang Sichuan Provincial Key Laboratory for Human Disease Gene Study Center for Medical GeneticsSichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences(2019RU026) Sichuan Academy of Medical SciencesChengduSichuan 610072China Department of Ophthalmology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China School of Ophthalmology and Optometry Wenzhou Medical CollegeWenzhouZhejiang 325035China Department of Organ Transplant Center Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Ophthalmology Peking University Third HospitalBeijing 100730China

The retinal pigment epithelium(RPE)and choroid are located behind the Human retina and have multiple functions in the Human visual system.Knowledge of the RPE and choroid cells and their gene expression profiles are fundamental for understanding retinal disease mechanisms and therapeutic strategies.Here,we sequenced the RNA of about 0.3 million single cells from Human RPE and choroids across two regions and seven ages,revealing regional and age differences within the Human RPE and choroid.Cell–cell interactions highlight the broad connectivity networks between the RPE and different choroid cell types.Moreover,the transcription factors and their target genes change during aging.The coding of somatic variations increases during aging in the Human RPE and choroid at the single-cell level.Moreover,we identified ELN as a candidate for improving RPE degeneration and choroidal structure during aging.The mapping of the molecular architecture of the Human RPE and choroid improves our understanding of the Human vision support system and offers potential insights into the intervention targets for retinal diseases.

关键词： Aging Choroid ELN Human RPE scRNA-seq

A COVID-19 T-Cell Response Detection Method Based on a Newly Identified Human CD8+T Cell Epitope from SARS-CoV-2—Hubei Province,China,2021

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China CDC weekly 2022年第5期4卷 83-87,I0005页

作者： Jie Zhang Dan Lu Min Li Maoshun Liu Sijia Yao Jianbo Zhan William J.Liu George F.Gao NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Savaid Medical School University of Chinese Academy of SciencesBeijingChina College of Laboratory Medicine and Life Sciences Wenzhou Medical UniversityWenzhouZhejiangChina Hubei Provincial Center for Disease Control and Prevention WuhanHubeiChina

Introduction:Similar to antibody detection,severe acute respiratory syndrome coronavirus type 2(SARSCoV-2)-specific T-cell response evaluation is also pivotal among the coronavirus disease 2019(COVID-19)convalescents and the vaccinated populations.Nucleocapsid(N)protein is one of the main structural proteins of SARS-CoV-2 and can trigger T-cell responses in Humans.Methods:An overlapping peptide pool covering the full length of the N protein was designed,peptides with positive T-cell activating potency in COVID-19 convalescents were screened,and CD8^(+)T cell epitopes were further identified.The epitope was used to detect the SARS-CoV-2-specific CD8^(+)T cell responses in COVID-19 convalescents based in intracellular cytokine staining and tetramer staining in flow cytometry.Results:A Human leukocyte antigen A(HLAA)*1101-restricted CD8^(+)T cell epitope,which could stimulate the production of IFN-γvia peripheral blood mononuclear cells(PBMCs)of the convalescents was defined,and the tetramer generated with this epitope could detect SARS-CoV-2-specific T cells in the PBMCs of the convalescents.The structural investigation eliminated that the epitope was a typical HLA-A^(*)1101-restricted T-cell epitope which was conserved among all the sarbecoviruses.Discussion:The newly identified SARS-CoV-2-derived T-cell epitope was helpful to detect the cellular immunity against different sarbecoviruses including SARS-CoV and SARS-CoV-2.This study provided an evaluation method and also a peptide candidate for the research and development of T-cell based vaccine for the virus.

关键词： restricted immunity Human

Human RNA Modifications Disease Database(HRMDD):A web resource for the molecular and clinical landscape of RNA modifications in Human diseases

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Genes & Diseases 2023年第4期10卷 1157-1160页

作者： Jianjian Wang Lifang Li Hanxiao Zhou Shuang Li Liting Tian Xinyue Wang Danyang Li Yue Yin Shangwei Ning Lihua Wang Department of Neurology The Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiang 150081China College of Bioinformatics Science and Technology Harbin Medical UniversityHarbinHeilongjiang 150081China

Here,we developed a comprehensive web-searchable database,designated as Human RNA Modifications Disease Database(HRMDD,http://***/HRMDD/home.jsp).RNA modification(RM)is an important mechanism of epigenetic regulation.With the evolution of both experimental technologies and computational methods,major progress has been made in identifying the genomic locations and distributions of various RM types throughout the transcriptome.^(1)

关键词： web Database Human

FOXP4 promotes proliferation of Human spermatogonial stem cells

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Asian Journal of Andrology 2023年第3期25卷 322-330页

作者： Shu-Wei Luo Le Tang Dai Zhou Hao Bo Li-Qing Fan Reproductive Medicine Center Hunan Provincial Maternal and Child Health Care HospitalChangsha 410000China NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care HospitalChangsha 410000China Institute of Reproduction and Stem Cell Engineering School of Basic Medicine ScienceCentral South UniversityChangsha 410000China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410000China

Continuous self-renewal and differentiation of spermatogonial stem cells(SSCs)is vital for maintenance of adult spermatogenesis.Although several spermatogonial stem cell regulators have been extensively investigated in rodents,regulatory mechanisms of Human SSC self-renewal and differentiation have not been fully established.We analyzed single-cell sequencing data from the Human testis and found that forkhead box P4(FOXP4)expression gradually increased with development of SSCs.Further analysis of its expression patterns in Human testicular tissues revealed that FOXP4 specifically marks a subset of spermatogonia with stem cell potential.Conditional inactivation of FOXP4 in Human SSC lines suppressed SSC proliferation and significantly activated apoptosis.FOXP4 expressions were markedly suppressed in tissues with dysregulated spermatogenesis.These findings imply that FOXP4 is involved in Human SSC proliferation,which will help elucidate on the mechanisms controlling the fate decisions in Human SSCs.

关键词： forkhead box P4 Human proliferation spermatogonial stem cells testis

Enterochromaffin Cell:Friend or Foe for Human Health?

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Neuroscience Bulletin 2023年第11期39卷 1732-1734页

作者： Yang Yu Yong-Chang Li Fu-Chao Zhang Guang-Yin Xu Jiangsu Key Laboratory of Neuropsychiatric Diseases and Institute of Neuroscience Soochow UniversitySuzhou 215123China

Enterochromaffin(EC)cells are a type of enteroendocrine cells that reside in the epithelial lining of the gastrointestinal tract.They function as sensory transducers of stimuli present in the intestinal lumen,as afferent nerves do not protrude into the lumen,but have been shown to act as a friend or a foe depending on various conditions.It has been well-described that EC cells play their role by releasing 5-HT to activate neighboring afferent nerves in a paracrine fashion,while emerging evidence suggests the existence of synaptic-like connections between enteroendocrine cells and adjacent afferent nerves based on in vitro co-culture and in vivo viral tracing studies.

关键词： nerves releasing Human